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Fermentation operation of gaseous substrates containing carbon monoxide and hydrogen

A gaseous substrate, fermentation method technology, applied in the field of fermenting gaseous substrates containing carbon monoxide (CO) and hydrogen (H2) to produce one or more alcohols, and can solve problems such as low cell density

Active Publication Date: 2016-05-25
巨鹏生物香港公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Priming with a batch inoculum from a stock culture ensures a healthy inoculum free of contaminants, but is not always successful as an inoculation procedure because the cell densities used are rather low, especially if in Rapid increases in method parameters such as gas and agitation rates immediately after inoculation

Method used

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  • Fermentation operation of gaseous substrates containing carbon monoxide and hydrogen

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Embodiment approach

[0072] One embodiment of the method of the present disclosure includes comprising carbon monoxide (CO) and hydrogen (H 2 ) is added to an aqueous medium in a bioreactor comprising an agitator; the aqueous medium contains one or more microorganisms; the method includes measuring CO conversion and H 2 conversion, and increasing the speed of the agitator in preselected speed steps in the range of 0 rpm to 200 rpm; where CO conversion is related to H 2 The difference in conversion rate is less than the specified conversion rate difference in the range of 0% to 25%. In one embodiment, the CO conversion exceeds the first CO conversion in the range of 0% to 25%. In one embodiment, H 2 Conversion over the first H in the range of 0% to 25% 2 Conversion rate. Measurement of CO conversion and H 2 The conversion rate and the action of increasing the speed of the agitator can be repeated. In one embodiment, CO conversion is higher than H 2 Conversion rate. In one embodiment, the CO...

Embodiment 1

[0097] Example 1: Clostridiumljungdahalii PETC: By comparing CO conversion with H 2 The difference in conversion is maintained at a predetermined value to increase the bacterial cell density in the reactor

[0098] The New Brunswick bioflow I reactor containing the fermentation medium initially contained 0.45 g / L of actively growing C. ljungdahlii. Before the start of the experiment, the stirring rate of the reactor was set to 100 rpm and the gas flow rate was set to 25 mL / min. Gas and liquid samples were taken from the reactor at intervals of 2 to 4 hours and analyzed for consumption or production of various gas components, broth acetic acid concentration, broth ethanol concentration, and optical density of the culture. Real-time measurement of syngas flow to the reactor. According to CO conversion and H 2 The conversion rate increases in a stepwise manner with the stirring of the reactor. The criteria for improving agitation are as follows: in the range of 100 rpm to 500...

Embodiment 2

[0111] Example 2: Clostridiumljungdahlii C-01: By comparing CO conversion with H 2 The difference in conversion is maintained at a predetermined value to increase the bacterial cell density in the reactor

[0112] A New Brunswick Bioflow I reactor containing about 1.5 liters (eg, in the range of about 1.32 liters to about 1.6 liters) fermentation medium initially contained about 0.28 g / L of actively growing C. ljungdahlii C-01 strain. Before the start of the experiment, the stirring rate in the bioreactor was set to about 100 rpm and the gas flow rate was set to about 30 mL / min. The temperature in the bioreactor was maintained in the range of about 36 to about 38°C throughout the experiment. The following samples were taken and analyzed at various time intervals (eg, about 2 hour intervals): syngas feed to the bioreactor, exhaust gas from the bioreactor, fermentation broth in the bioreactor. Sample analysis provides: consumption of various gas components, production of vario...

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Abstract

The present invention relates to a gaseous substrate fermentation method comprising: adding a gaseous substrate comprising carbon monoxide (CO) and hydrogen (H2) to an aqueous medium in a bioreactor; said method comprising measuring CO conversion; measuring H2 conversion; increasing the flow of the gaseous substrate by a preselected flow coefficient; wherein agitation includes greater than or equal to a target agitation rate.

Description

technical field [0001] The present disclosure relates generally to compounds containing carbon monoxide (CO) and hydrogen (H 2 ) fermentation method of gaseous substrates. Specifically, the present invention relates to fermentation containing carbon monoxide (CO) and hydrogen (H 2 ) of a gaseous substrate to produce one or more alcohols. Background technique [0002] Production of chemical substances such as organic substances by fermentation of microorganisms from gaseous substrates containing carbon monoxide and hydrogen in a medium containing suitable nutrients and trace minerals using certain bacteria such as those from the genus Clostridium Methods for acids such as acetic acid and alcohols such as ethanol have been demonstrated. For example, US Patent No. 5,173,429 to Gaddy et al. discloses Clostridiumljungdahlii ATCC No. 49587, an anaerobic microorganism for the production of ethanol and acetate from synthesis gas. US Patent No. 5,807,722 to Gaddy et al. discloses...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P7/54
CPCC12P7/065C12M21/04C12M27/02C12M29/04C12M29/18Y02E50/10C12Q3/00
Inventor 瑞安·塞纳拉特纳希罗娜·斯科特青-焕·辜
Owner 巨鹏生物香港公司