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Method for regulating size and grain weight of rice seeds

A rice grain and grain weight technology, applied in the field of genetic engineering, can solve the problems of increased glume, increased glume cells, increased grain width and grain weight, etc., to achieve enhanced resistance, high economic benefits, improved seed size and effect of grain weight

Active Publication Date: 2015-03-18
江苏省高科种业科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Loss of GW2 function increases the number of glume cells, resulting in larger glumes, increased grain width, and grain weight

Method used

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  • Method for regulating size and grain weight of rice seeds
  • Method for regulating size and grain weight of rice seeds
  • Method for regulating size and grain weight of rice seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]Example 1 OsABC1-2 Obtaining and phenotypic analysis of overexpression transgenic lines

[0032] 1) OsABC1-2 Construction of overexpression vector

[0033] RNAsimple Total RNA Extraction Kit (Tiangen, DP419) was used to extract total RNA from leaves of japonica rice variety Zhonghua 11, and reverse-transcribed into cDNA using PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa, 6110A) kit. The steps of reverse transcription are as follows: Take 3 μg of RNA sample and add 50 μmol L -1 OligodT primer 1μl and 10mmol L -1 dNTP mixture 1μl, with RNase free dH 2 Make up the volume with O to 10 μl, keep warm at 65°C for 5 minutes, and cool rapidly on ice; add 5×PrimeScript Buffer 4 μl and 40U μl to the denatured reaction solution respectively -1 RNase Inhibitor 0.5μl, 200U μl -1 PrimeScript RTase 1 μl with RNase free dH 2 O make up the volume to 20 μl, mix slowly; carry out the reverse transcription reaction according to the following conditions: 42°C for 60 minutes,...

Embodiment 2

[0054] Example 2 OsABC1-2 Resistance analysis of overexpressed transgenic lines under dark treatment

[0055] Seeds of wild-type Zhonghua 11 and overexpressed transgenic strains were germinated on MS medium, and 50 mg L of overexpressed strains were added to the medium -1 Hygromycin was screened. After 2 weeks of growth in medium, the seedlings were transferred to small pots filled with soil to continue growing. 4-week-old plants were used for dark treatment. After 72 hours of dark treatment in an incubator at 26°C, leaves of Zhonghua 11 and overexpression plants showed a certain degree of yellowing phenotype. However, compared with the control, the leaves of the overexpressed plants remained green and had strong vitality ( Figure 4 ). Further measurement of leaf chlorophyll fluorescence parameters by PAM2100 chlorophyll fluorescence instrument showed that the maximum photochemical quantum yield (Fv / Fm) and the actual photosynthetic quantum yield (Yield) of PS Ⅱ in the ...

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Abstract

The invention discloses a method for regulating rice grain size and grain weight, which comprises the steps of constructing an OsABC1-2 gene overexpression vector and obtaining a transgenic plant with OsABC1-2 overexpression. The present invention significantly increases the seed size and grain weight and enhances the resistance to dark treatment-induced leaf senescence by overexpressing the OsABC1-2 gene in japonica rice. Therefore, the gene is a new regulatory factor for rice grain shape traits, and has potential industrial application value in improving rice seed size and grain weight and increasing crop yield, resulting in higher economic benefits.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for regulating the size and weight of rice grains. Background technique [0002] Rice is one of the most important food crops, feeding more than half of the world's population. With the growth of population and the improvement of living standards, people put forward higher and higher requirements for rice both in terms of yield and quality. It is estimated that by 2025, the global population will exceed 8 billion, and the number of people whose staple food is rice will increase to 3.5 billion. According to the most conservative estimates, the increase in rice demand due to population growth is at least 300 million tons. The increase of rice yield depends on the potential of expanding the planting area is very limited. Due to the limitation of water resources and arable land area, coupled with the advancement of economic development and urbanization, the ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H4/00A01H5/00
Inventor 高清松杨立明刘廷武张云峰
Owner 江苏省高科种业科技有限公司
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