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Method for extracting and separating myricetin from bayberry tree

A bayberry and bark technology, applied in the field of extraction and separation of flavonoids, can solve the problems of few studies on extraction and separation, and achieve the effects of high product yield, low production cost and simple process realization.

Active Publication Date: 2015-09-30
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Myricetin is one of the main active components in bayberry bark, and there are few reports on its extraction and isolation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1] extract

[0025] Get the bayberry bark raw material, extract 3 times in 50 ℃ environment with 95% ethanol of 3 times of bayberry bark raw material quality, each extraction time is 2 hours;

[0026] 2] concentrated

[0027] The extracts extracted in step 1 were combined and concentrated to a specific gravity of 1.03, left for 12 hours, precipitated, and centrifuged.

[0028] 3] 1 precipitation

[0029] The precipitate obtained above was extracted with dichloromethane equivalent to 50 times the mass of the precipitate under the condition of 50° C., stirred and extracted twice for 0.5 h each time, and filtered to obtain a precipitate.

[0030] 4] Crystallization

[0031] Dissolve the precipitate obtained above with 3 times the mass and 80% ethanol, and add 5% of the weight of the precipitate to decolorize at 60°C for 0.5h; Place the crystals for 24 hours, filter, and dry the crystals to obtain the finished product. The purity of the obtained product is 98.1%, the yi...

Embodiment 2

[0033] 1] extract

[0034] Get the bayberry bark raw material, extract 3 times in 90 ℃ environment with the water of 5 times of bayberry bark raw material quality, each extraction time is 1.5 hours;

[0035] 2] concentrated

[0036] The extracts extracted in step 1 were combined and then concentrated to a specific gravity of 1.30, left for 24 hours to produce a precipitate, and centrifuged.

[0037] 3] 1 precipitation

[0038] The precipitate obtained above was extracted with acetone equivalent to 10 times the mass of the precipitate under the condition of 40° C., stirred and extracted twice for 1 hour each time, and filtered to obtain a precipitate.

[0039] 4] Crystallization

[0040] Dissolve the precipitate obtained above with 8 times the mass of methanol at a concentration of 60%, and add activated carbon of 6% of the weight of the precipitate to decolorize at 50°C for 0.5h; Place the crystals in the middle for 24h, filter, and dry the crystals to obtain the finished ...

Embodiment 3

[0042] 1] extract

[0043] Get bayberry bark raw material, extract 3 times with 80% ethanol of 5 times of bayberry bark raw material quality in 60 ℃ environment, each extraction time is 1.5h hour;

[0044] 2] concentrated

[0045] Combine the extracts extracted in step 1 and concentrate to a specific gravity of 1.08, leave it for 10 hours to produce a precipitate, centrifuge

[0046] 3] 1 precipitation

[0047] The precipitate obtained above was extracted with ethyl acetate equivalent to 10 times the mass of the precipitate at 25° C., stirred and extracted twice for 1 hour each time, and filtered to obtain a precipitate.

[0048] 4] Crystallization

[0049] Dissolve the precipitate obtained above with 3 times the mass of ethanol at a concentration of 92%, and add 13% of the weight of the precipitate to decolorize at 50°C for 1.5 hours; Place the crystals for 24 hours, filter, and dry the crystals to obtain the finished product. The purity of the obtained product is greater...

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PUM

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Abstract

The invention provides a method for extracting and separating myricitrin from waxberry tree. The method comprises the following steps: (1), extracting; (2), concentrating; (3), primary sedimentation, wherein obtained sediments are stirred and extracted for 1-3 times at 0 DEG C-60 DEG C by using a first solvent which is 1-50 times the weight of the sediments, and filtering to obtain primary sediments, where each stirring and extracting process lasts for 0.5-3 hours, and then are filtered; (4), crystallizing, wherein the obtained primary sediments are dissolved by a second solvent which is 3-10 times the weight of the primary sediments, the second solvent is methyl alcohol or alcohol, and active carbon which is 1%-20% of the weight of the primary sediments is added for discoloring for 0.5-4 hours in an environment with temperature being 0 DEG C-80 DEG C; and filtering after the discoloring is completed, standing the filtrate for 24 hours in an environment with temperature being 0-30 DEG C, filtering, crystallizing and drying to obtain a finished product. The method disclosed by the invention is simple and effective and low in production cost; moreover, column separation is avoided, the process is simple and convenient to realize, the product yield is high and the recovery rate of final effective ingredients of the product is high, so that the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a technology for extracting and separating flavonoids, in particular to a method for extracting and separating myricetin from bayberry bark. Background technique [0002] Myricitrin exists in the bark and leaves of Myrica rubra Sieb. et Zucc. It has the effects of constricting blood vessels, raising blood pressure, exciting the heart, promoting choleresis, anti-inflammation, and mutagenicity. Its inhibitory effect on ocular aldose reductase may be useful in the treatment of diabetic cataracts. [0003] Myricetin is one of the main active components in bayberry bark, and there are few reports on its extraction and separation. Contents of the invention [0004] The invention provides a method for extracting myricitrin from bayberry bark. The method has simple process, high yield and low cost, and is suitable for industrial production. [0005] Basic solution of the present invention is as follows: [0006] The method for ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 肖红王晓莹张瑜
Owner SHAANXI JIAHE PHYTOCHEM