Alcohol dehydrogenase embedded gelatin-silica hybrid gel and preparation method thereof

An alcohol dehydrogenase, hybrid gel technology, applied in the direction of immobilization on or in an inorganic carrier, immobilized on/in an organic carrier, etc., can solve the problems of low stability and storage stability, and enzyme molecules. Easy to leak, easy to swell the gel and other problems, to achieve the effect of simple and easy preparation process, good stability and low swelling degree

Inactive Publication Date: 2013-12-25
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The usual problem with gelatin gel immobilized enzymes is that the carrier has a large pore size and the enzyme molecules are easy to leak
The gel is easy to swell, the mechanical strength is poor, and the cycle stability and storage stability are not high

Method used

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  • Alcohol dehydrogenase embedded gelatin-silica hybrid gel and preparation method thereof
  • Alcohol dehydrogenase embedded gelatin-silica hybrid gel and preparation method thereof
  • Alcohol dehydrogenase embedded gelatin-silica hybrid gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dissolve 706mg of gelatin in 4mL of deionized water to prepare a gelatin solution with a mass fraction of 15%, add 4mg of alcohol dehydrogenase to the above gelatin solution, stir, mix well, and prepare 20 mL of sodium silicate precursor with a concentration of 40mM Use concentrated hydrochloric acid to adjust the pH of the sodium silicate solution to 6.5, take 40 μL of a 50% glutaraldehyde solution and add it to the sodium silicate solution to obtain a precursor mixed solution, and then use a syringe to mix gelatin and alcohol dehydrogenase The mixed solution was added dropwise to deionized water at 0°C to generate gelatin gel particles embedding alcohol dehydrogenase. After filtering and washing three times with deionized water, the gelatin gel particles embedding alcohol dehydrogenase were added to prepare The sodium silicate precursor mixed solution was incubated for 25 minutes to prepare a gelatin-silica hybrid gel embedding alcohol dehydrogenase.

[0020] The gela...

Embodiment 2

[0025] Dissolve 1 g of gelatin in 3 mL of deionized water to prepare a gelatin solution with a mass fraction of 25%, add 12 mg of alcohol dehydrogenase to the above gelatin solution, stir, mix well, and prepare 20 mL of sodium silicate precursor with a concentration of 80 mM Use concentrated hydrochloric acid to adjust the pH value of the sodium silicate solution to 8, take 50% glutaraldehyde solution and add 100 μL glutaraldehyde to the sodium silicate solution to obtain a precursor mixed solution, and then use a syringe to remove the gelatin from the alcohol. The mixed solution of hydrogenase was added dropwise to deionized water at 4°C to generate gelatin gel particles embedding alcohol dehydrogenase. After filtering and washing with deionized water three times, the gelatin gel particles embedding alcohol dehydrogenase Add the prepared mixed solution of sodium silicate precursor and incubate for 35 minutes to prepare gelatin-silicon oxide hybrid gel embedding alcohol dehydro...

Embodiment 3

[0027] Dissolve 1g of gelatin in 3mL of deionized water to prepare a gelatin solution with a mass fraction of 25%, add 12mg of alcohol dehydrogenase to the above gelatin solution to make the concentration 4mg / mL, stir, mix well, and prepare 20 mL The sodium silicate precursor solution with a concentration of 50mM, adjust the pH value of the sodium silicate solution to 7.0 with concentrated hydrochloric acid, take a 50% glutaraldehyde solution by mass fraction and add 40 μL glutaraldehyde to the sodium silicate solution to obtain a precursor mixed solution , and then use a syringe to drop the mixed solution of gelatin and alcohol dehydrogenase into deionized water at 4 ℃ to generate gelatin gel particles embedded with alcohol dehydrogenase. After filtering, wash with deionized water three times, the The gelatin gel particles embedded with alcohol dehydrogenase were added to the prepared mixed solution of sodium silicate precursor and incubated for 30 minutes to prepare gelatin-s...

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Abstract

The invention discloses an alcohol dehydrogenase embedded gelatin-silica hybrid gel and a preparation method thereof. According to invention, the grain diameter of the alcohol dehydrogenase embedded gelatin-silica hybrid gel is 3-4mm, and a silica coating is coated on a gelatin core. The preparation method comprises the following steps of: preparing a mixed solution of gelatin and alcohol dehydrogenase; preparing a mixed solution of sodium silicate and a glutaraldehyde solution precursor; adding the mixed solution of gelatin and alcohol dehydrogenase into deionized water at a temperature of 0-4 DEG C droplet by droplet to generate alcohol dehydrogenase embedded gelatin gel particles; stirring and incubating to obtain the alcohol dehydrogenase embedded gelatin-silica hybrid gel. The preparation method has the advantages that the preparation condition is mild, the preparation process is simple and feasible, and the alcohol dehydrogenase embedded gelatin-silica hybrid gel has a strong fixing capability; compared with gelatin gel, the hybrid gel has a lower leakage rate of immobilized alcohol dehydrogenase, and lower swelling degree and better recycling stability.

Description

technical field [0001] The invention relates to a gelatin-silicon oxide hybrid gel embedding alcohol dehydrogenase and a preparation method thereof, belonging to enzyme immobilization technology. Background technique [0002] Gelatin gel is a commonly used carrier for immobilized enzymes. At present, the gelatin gel immobilized enzyme mainly adopts the embedding method. The embedding method has mild conditions and good biocompatibility, which is conducive to maintaining the structural integrity of the enzyme, thereby improving the activity maintenance rate of the enzyme, and the carrier has a large pore size, which is conducive to the transfer of reactants and products. [0003] The usual problem with gelatin gel-immobilized enzymes is that the carrier has a large pore size and the enzyme molecules are easy to leak. The gel is easy to swell, has poor mechanical strength, and low cycle stability and storage stability. The solution to the problem is usually to use a bifunc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/04
Inventor 吴洪宋晓凯姜忠义王晓莉
Owner TIANJIN UNIV
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