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Human blood lipid (serum/plasma) quality management reference kit and preparation method

A quality management and kit technology, applied in the biological field, can solve problems such as inability to use quality comparison investigation and quality control

Active Publication Date: 2016-03-23
上海北加生化试剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it cannot be used for quality comparison investigation and quality control between detection systems and assay kits of different brands

Method used

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  • Human blood lipid (serum/plasma) quality management reference kit and preparation method
  • Human blood lipid (serum/plasma) quality management reference kit and preparation method
  • Human blood lipid (serum/plasma) quality management reference kit and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The present invention is a human blood lipid (serum / plasma) quality management reference kit, which contains a reference reagent, and the reference reagent is based on human serum (plasma), and is added with Contains human-derived blood lipid components, a stabilizing system and a preservative system. The human-derived blood lipid components are human-derived apolipoprotein A1 antigen (APOA1), apolipoprotein B antigen (APOB), high-density lipoprotein cholesterol (HDL ), low-density lipoprotein cholesterol (LDL), lipoprotein a (La), cholesterol (CHOL) and triglyceride (TG) any one or a combination of more than one, the stable system is Triton series surface Active agent, Tween series surfactant, EDTA series surfactant, polyvinyl alcohol series polymer, polyvinylpyrrolidone series polymer, polyethylene glycol series polymer and phosphate buffer,

[0039] Described stabilizer is made up of TritonX100, Tween20, EDTA, polyvinyl alcohol, polyvinylpyrrolidone, polyethylene gly...

Embodiment 2

[0046] The present invention is a human blood lipid (serum / plasma) quality management reference kit, which contains a reference reagent, and the reference reagent is based on human serum (plasma), and is added with Contains human-derived blood lipid components, a stabilizing system and a preservative system. The human-derived blood lipid components are human-derived apolipoprotein A1 antigen (APOA1), apolipoprotein B antigen (APOB), high-density lipoprotein cholesterol (HDL ), low-density lipoprotein cholesterol (LDL), lipoprotein a (La), cholesterol (CHOL) and triglyceride (TG) any one or a combination of more than one, the stable system is Triton series surface Active agent, Tween series surfactants, EDTA series surfactants, polyvinyl alcohol series polymers, polyvinylpyrrolidone series polymers, polyethylene glycol series polymers and any one or more than one of phosphate buffer The combination.

[0047] Further, the stabilizer is composed of TritonX100, Tween40, and polyv...

Embodiment 3

[0055] The present invention is a human blood lipid (serum / plasma) quality management reference kit, which contains a reference reagent, and the reference reagent is based on human serum (plasma), and is added with Contains human-derived blood lipid components, a stabilizing system and a preservative system. The human-derived blood lipid components are human-derived apolipoprotein A1 antigen (APOA1), apolipoprotein B antigen (APOB), high-density lipoprotein cholesterol (HDL ), low-density lipoprotein cholesterol (LDL), lipoprotein a (La), cholesterol (CHOL) and triglyceride (TG) any one or a combination of more than one, the stable system is Triton series surface Active agent, Tween series surfactants, EDTA series surfactants, polyvinyl alcohol series polymers, polyvinylpyrrolidone series polymers, polyethylene glycol series polymers and any one or more than one of phosphate buffer The combination.

[0056] Further, the stabilizer is composed of Tween80, EDTA-Na4, polyvinyl a...

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Abstract

The invention provides a human blood fat (serum / plasma) quality management reference kit. The kit takes human serum (plasma) as a matrix; a human blood fat component (human apolipoprotein A1 antigen, apolipoprotein B antigen, high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL) and the like), a stable system and an anticorrosion system are added to the matrix; the stable system is any combination of a surfactant of Triton series, Tween series, EDTA (ethylene diamine tetraacetic acid) series and the like, a polymer of polyvinyl alcohol series, polyvinylpyrrolidone series, polyethylene glycol series and the like, and a phosphate buffer solution; and the anticorrosion system is any combination of sodium azide, gentamicin, amphomycin and ProClin series. The invention further provides a preparation method of the kit. The kit is free from medium effect interference, is used for quality control and evaluation between the detection systems, and more really reflects actual quality conditions of human serum (plasma) samples, which are detected by detection systems.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit and a preparation method thereof, in particular to a human blood lipid (serum / plasma) quality management reference kit and a preparation method thereof. Background technique [0002] Apolipoprotein A1 antigen, apolipoprotein B antigen, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, lipoprotein a, cholesterol and triglyceride are the most commonly used test items in clinical biochemical lipid metabolism testing The screening and routine inspection of cardiovascular risk factors have important clinical detection value. Therefore, quality control during the testing process and the consistency of test results from different laboratories are very important. [0003] According to the quality control requirements, the composition and matrix effect of the quality control product (investigation product) used are as close as possible to the compos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/96
Inventor 张瑞镐刘中令刘颖冰朱晓敏
Owner 上海北加生化试剂有限公司
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