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Method for combined extraction of tritepenoidic acid, polyphenols and polysaccharides in loquat flower

A technology of combined extraction and loquat flowers, applied in the directions of pharmaceutical formulations, steroids, preparations for skin care, etc., can solve problems such as not being able to be utilized to the greatest extent, and achieve good economic benefits, strong operability, and high value.

Active Publication Date: 2014-12-17
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, when extracting active ingredients, only one of them was extracted, so it could not be utilized to the greatest extent.

Method used

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  • Method for combined extraction of tritepenoidic acid, polyphenols and polysaccharides in loquat flower
  • Method for combined extraction of tritepenoidic acid, polyphenols and polysaccharides in loquat flower
  • Method for combined extraction of tritepenoidic acid, polyphenols and polysaccharides in loquat flower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The extraction of loquat flower triterpene acid

[0031] The waste from processing loquat scented tea is dried, pulverized, and passed through a 30-mesh sieve for later use. Weigh 1g of the powder, add ethanol solution to the extraction bottle of the microwave extraction instrument for microwave-assisted extraction, filter after the extraction, and concentrate the filtrate to dryness under reduced pressure, dissolve the extract in methanol and constant volume, and inject it into the high-efficiency liquid phase Chromatographic determination of ursolic acid and oleanolic acid, the determination method is shown in Test Example 1. Carry out microwave-assisted extraction according to the conditions in the following orthogonal table (see Table 1), use high-performance liquid chromatography to determine ursolic acid (UA) and oleanolic acid (OA) in the extract, and optimize triterpenoids according to comprehensive indicators Acid extraction process parameters. Ac...

Embodiment 3

[0046] Embodiment 3 The extraction of polysaccharide in loquat flower

[0047] After slightly drying the residue extracted in the second stage, add water, extract in a microwave extractor, filter after extraction, and measure the polysaccharide content in the filtrate. Perform microwave-assisted extraction according to the conditions in the following orthogonal table (see Table 5), and optimize the process parameters for extraction of flavonoids and polyphenols. The experimental results are shown in Table 6.

[0048] Table 5 Orthogonal table of polysaccharide extraction experiments

[0049]

[0050] Table 6 Orthogonal test results of polysaccharide extraction

[0051]

[0052] Through the orthogonal test range analysis, the best combination A 3 B 3 C 3 , under this condition, the extraction rate of polysaccharides was 4.6 mg / g, so this combination was selected as the optimal process conditions, that is, the extraction temperature was 80°C, the liquid-solid ratio wa...

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Abstract

The invention relates to a method for the combined extraction of tritepenoidic acid, polyphenols and polysaccharides in a loquat flower. The method comprises the following steps: (1) drying the loquat flower raw material until the water content is equal to smaller than 5%, and grinding and screening by a 30-meshes screen for standby; (2) degreasing; (3) extracting the tritepenoidic acid; (4) extracting total flavonoids; (5) extracting the polysaccharides. The method, provided by the invention, makes full use of wastes left during the process of loquat flower tea processing, so that effective components in the wastes are used, the tritepenoidic acid, the polyphenols and the polysaccharides in the loquat flower are subjected to combined extraction, so that the effective components in the tritepenoidic acid, the polyphenols and the polysaccharides are extracted in a staged and stepped manner, and the condition that only one of the tritepenoidic acid, the polyphenols and the polysaccharides is extracted is changed. The method provided by the invention is simple in process, safe and environment-friendly, low in cost, strong in operability, and easy for practical production and application.

Description

technical field [0001] The invention relates to the technical field of extraction and purification of active ingredients of agricultural by-products, in particular to a method for jointly extracting multiple active ingredients from loquat flowers. Background technique [0002] Loquat flower is Rosaceae loquat ( Eriobotrya japonica Lindl ) flowers, warm in nature, light in taste, particularly delicate fragrance, with sweet taste, containing total flavonoids and triterpene saponins, etc., have the effects of reducing phlegm, relieving cough and clearing lung. Quenches thirst and lowers qi, sharpens lung qi, relieves vomiting and nausea, clears upper burnt heat, moistens five viscera", folks are often used to treat children with lung-heat cough, and elderly patients with chronic cough that does not heal. During the planting process of loquat, 80%~90% of the branches of the whole loquat tree can form flower spikes, and each flower spike has 70~250 flowers, but only 5%~10% of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61K8/97A61Q19/08A61Q19/00C07J63/00C08B37/00
Inventor 郑美瑜陆胜民夏其乐汪文浩
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES