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Electrochemical analyzing method for constructing 17beta-estradiol aptamer sensor based on dendritic gold modification BDD electrode

An aptamer sensor and electrochemical analysis technology, applied in the field of electrochemical analysis, can solve the problems of complicated operation and low sensitivity, and achieve the effects of simple preparation method, low background current, and simple and easy method.

Inactive Publication Date: 2014-01-01
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide an electrochemical analysis of a 17β-estradiol aptamer sensor based on a nano-dendritic gold-modified BDD electrode for the shortcomings of the existing detection 17β-estradiol technology, such as low sensitivity and complicated operation. Method, by modifying the base electrode with nano-dendritic gold with a special structure, the electrode conductivity and the ability to load aptamers are improved, and the highly sensitive and selective detection of 17β-estradiol is realized, and the detection sensitivity reaches 5×10 -15 mol / L, has the advantages of simple preparation method, easy operation, and short time consumption

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  • Electrochemical analyzing method for constructing 17beta-estradiol aptamer sensor based on dendritic gold modification BDD electrode

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Embodiment 1

[0025] (1) An electrochemical analysis method for a 17β-estradiol aptamer sensor that modifies a boron-doped diamond film (BDD) substrate electrode with a dendritic gold nanostructure with a special morphology, and its working electrode is obtained by the following steps:

[0026] The BDD electrode soaked and pretreated in aqua regia was used as the working electrode, the platinum wire electrode was used as the auxiliary electrode, the saturated calomel electrode was used as the reference electrode, and 1 mol / L H 2 SO 4 The solution is an electrolyte, and the i-t method is used to apply a voltage of 3.2V to the electrode system and keep it for 30 min. After that, the BDD electrode is rinsed with deionized water and dried in the air to obtain a BDD electrode with a clean surface; L ZnCl 2 , 3 mol / L KCl and 0.5 mol / L H 3 BO 3 In the mixed solution (pH=4.5), galvanostatic deposition was carried out for 30 s, and the current density was 0.13 A / cm2; after the deposition, the ob...

Embodiment 2

[0037] The prepared nucleic acid aptamer sensor was used to analyze the content of 17β-estradiol in actual water samples.

[0038] The actual water samples were taken from a residential area on Siping Road, Yangpu District, Shanghai, China. Water samples were filtered through a 0.22 μm filter membrane for detection. After the water sample was diluted 25 times with Tris-HCl buffer solution, the [Fe(CN) 6 ] 4- / [Fe(CN) 6 ] 3- (containing 0.1 mol / L KCl), incubate with the sensor at room temperature for 15 min, then use the platinum wire electrode as the counter electrode and the saturated calomel electrode as the reference electrode, and perform electrochemical impedance detection on the CHI660c electrochemical workstation. , to obtain the change rate of the impedance of the sensor caused by the water sample. The content of 17β-estradiol contained in the water sample can be calculated back from the sensor sensitivity curve obtained in Example 1. The experiment shows that th...

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Abstract

The invention relates to an electrochemical analyzing method for constructing a 17beta-estradiol aptamer sensor based on a dendritic gold modification boron-mixed diamond film BDD electrode and provides an electrochemical analyzing method high in sensitivity and high in selectiveness. The method comprises the steps of manufacturing special-morphology nanometer dendritic gold / BBD electrodes based on an electrochemistry method and further performing nucleic acid aptamer load assembly by adopting the surface characteristics of the modified electrode to construct the 17beta-estradiol aptamer sensor, wherein the sensor is used for detecting environmental internal-secretion interfering-substance, namely, 17beta-estradiol. Compared with the prior art, a dendritic gold structure with multiple levels of active sites is constructed on the BDD substrate electrode with a simple and novel double-template method, a nucleic acid aptamer capable of being combined with target substances in a peculiar mode is adopted at the same time, noise in the analyzing process is effectively lowered, the detection sensitivity and selectiveness of the sensor are greatly improved, the detection limit reaches 5*10<-15> mol / L, and the sensor has good reproducibility.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials, electrochemical analysis and environmental detection, in particular to an electrochemical analysis method for constructing a 17β-estradiol aptamer sensor on a dendritic gold-modified boron-doped diamond film (BDD) electrode. Background technique [0002] Endocrine disruptors (EDCs) are a class of artificial or naturally occurring exogenous estrogens that have similar functions to endogenous estrogens. They can interfere with the normal behavior of organisms and the synthesis, storage, secretion, transport, combination and clearance of normal hormones in the body, leading to various dysfunctions in the organism. Such as causing damage to biological reproductive function, population decline, human male and female birth ratio imbalance, inducing various cancers, etc. Therefore, the effective detection of endocrine disruptors is of great significance to the protection of the ecological enviro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
Inventor 刘梅川柯红阳赵国华庄璐
Owner TONGJI UNIV
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