Bacillus subtilis strain and method for gamma-PGA (poly-glutamic acid) by utilizing same

A technology of Bacillus subtilis and microorganisms, which is applied in the field of producing γ-PGA strains, can solve the problems of bacterial contamination, high cost, liquid escape, etc., and achieve the effects of inhibiting foam generation, improving production efficiency, and avoiding liquid escape

Active Publication Date: 2014-01-08
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation production of γ-PGA by existing strains has the defects of long cycle, low efficiency, and high cost. In addition, there are reports that lipopeptide biosurfactants will be produced in the liquid fermentation stage during the production of γ-PGA by using existing strains. By-products (Qijun Wang, et al., Co-producing lipopeptides an...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The invention provides a kind of Bacillus subtilis used for the fermentation production of γ-PGA, which is named after Bacillus subtilis HNCL 1266, the strain has been preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on July 22, 2013, and the preservation number is CGMCC No.7949.

[0019] In order to make it easier for those skilled in the art to obtain the strains provided by the present invention, the inventors will briefly describe the process of obtaining the strains of the present invention below.

[0020] Bacillus subtilis obtained by the present invention Bacillus subtilis HNCL 1266 is obtained from the screened γ-PGA producing strain Bacillus subtilis HD11 (Zhu Lijuan et al., Identification and mutation selection of high-yield strains of γ-polyglutamic acid, Anhui Agricultural Science Bulletin, 2012, 18(4): 8-10), through atmospheric room temperature plasma (ARTP) (see Chinese patent 2008200793821) ob...

Embodiment 2

[0040] using the filtered Bacillus subtilis The method for producing γ-PGA by liquid fermentation of HNCL 1266, the specific production steps are as follows:

[0041] (1) the strain Bacillus subtilis HNCL 1266 was inserted into the sterile seed medium, and fermented and cultured at 30-40°C for 5-20 hours under aerobic conditions; the composition of the seed medium was: glucose 10-50g / L, nitrogen source 1-10g / L, glutamine Sodium sulfate 1-20g / L, magnesium sulfate 0.1-10g / L, dipotassium hydrogen phosphate 1-20g / L, prepared with water, medium pH 4-9.

[0042] (2) Put 3L of medium in a 5L fermenter, and the medium formula used is: 100 g / L of glucose, 15 g / L of yeast extract, 40 g / L of sodium glutamate, 10 g / L of sodium chloride, Magnesium sulfate 5 g / L, dipotassium hydrogen phosphate 5 g / L, adjust pH 7.0;

[0043] Add 1g of foam enemy before the medium is sterilized. The foam enemy is produced by Nanzhao County Auxiliary Factory of Henan Province, and the production batch num...

Embodiment 3

[0048] Production parameters are the same as Example 2 , only the added amount of foam enemy was reduced to 0.5g.

[0049] It was observed that there was a small amount of foam during the fermentation process, but there was no need to add foam enemy. After measurement, the yield of γ-PGA was about 35g / L.

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Abstract

The invention belongs to the technical field of producing gamma-PGA (poly-glutamic acid) by utilizing microorganisms, and particularly relates to a production strain for producing the gamma-PGA, and a production method for producing the gamma-PGA by utilizing the strain. The bacillus subtilis HNCL1266 strain is preserved the China General Microbiological Culture Collection Center (CGMCC), with the number of CGMCC No.7949. The production method for fermenting and producing the gamma-PGA by utilizing the strain comprises the steps: firstly, inoculating the strain into a seed medium for fermentation, then inoculating the fermented culture medium for fermentation according to a proportion, and finally extracting and preparing gamma-PGA, and the like. The bacillus subtilis strain is utilized for producing gamma-PGA, the maximum gamma-PGA accumulation amount can reach 50g/L within 24 hours, and compared with the production strain adopted in the prior art, foam generation can be effectively inhibited only by adding less defoamer GPE in an initial culture medium, liquid escape can be avoided in the fermentation process so as to facilitate the realization of industrial production.

Description

Technical field [0001] The present invention is the field of production of γ-PGA technology using microbial biological, which involves a strain that produces γ-PGA and the use of the strain to produce γ-PGA. Background technique [0002] γ-pga, γ-polyaline is a water-soluble polymer amino acid polymer formed by the combination of L-glutamic acid and D-glutamic acid. It has thickening, emulsification, gelFilm, moisturizing, non -toxic, biodegradable and other performances can be widely used in the fields of pharmaceutical, agriculture, food and other fields, and have great development value and application prospects. [0003] In existing technologies, γ-PGA mainly uses Bacteria to be a microorganism of microorganisms Bacillus Licheniformis ATCC9945A, Bacillus Licheniformis P-104, Bacillus subtilis Ifo3335, Bacillus subtilis Tam-4, Bacillus subtilis F02-1, etc., is produced by liquid fermentation.However, the existing strains are fermented and produced in the γ-PGA. There are short...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/02C12R1/125
Inventor 陈双喜雷雪梅张乐乐杜沛朱丽娟张二超
Owner HENAN UNIVERSITY
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