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Nutrient, continuous culture method and continuous culture device for immobilized aerobic microorganisms

A technology of aerobic microorganisms and nutrients, applied in enzymology/microbiology devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve problems such as unsatisfactory technical means and limited cultivation effects, and improve domestication efficiency , shorten the recovery time of the activity, increase the effect of the effect

Inactive Publication Date: 2016-03-30
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, before the immobilized nitrifying bacteria particles are put into the actual wastewater treatment system, it is necessary to carry out continuous domestication and cultivation of the immobilized microbial particles to restore their activity. However, the current technical means for continuous domestication and cultivation of the immobilized microbial particles is not ideal enough, and the cultivation effect is limited.

Method used

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  • Nutrient, continuous culture method and continuous culture device for immobilized aerobic microorganisms

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Effect test

Embodiment 1

[0023] In this embodiment, a nutrient for immobilizing aerobic microorganisms is composed of the following components according to weight percentage:

[0024] Glucose is 0.1%, ammonium chloride is 0.5%, magnesium sulfate is 0.05%, sodium chloride is 0.05%, calcium chloride is 0.01%, potassium dihydrogen phosphate is 0.005%, potassium chloride is 0.005%, iron sulfate is 0.001%, sodium bicarbonate is 1%, water is 98.28%.

[0025] The nutrient for the immobilized aerobic microorganisms of this embodiment can be obtained by uniformly mixing the above-mentioned raw material components.

Embodiment 2

[0027] In this embodiment, a nutrient for immobilizing aerobic microorganisms is composed of the following components according to weight percentage:

[0028] Glucose is 0.2%, ammonium chloride is 2.5%, magnesium sulfate is 0.1%, sodium chloride is 0.07%, calcium chloride is 0.07%, potassium dihydrogen phosphate is 0.025%, potassium chloride is 0.025%, iron sulfate is 0.001%, sodium bicarbonate is 2%, water is 95.01%.

[0029] The nutrient for the immobilized aerobic microorganisms of this embodiment can be obtained by uniformly mixing the above-mentioned raw material components.

Embodiment 3

[0031] In this embodiment, a nutrient for immobilizing aerobic microorganisms is composed of the following components according to weight percentage:

[0032] Glucose is 0.1%, ammonium chloride is 3%, magnesium sulfate is 0.05%, sodium chloride is 0.08%, calcium chloride is 0.05%, potassium dihydrogen phosphate is 0.01%, potassium chloride is 0.01%, ferric sulfate is 0.05%, sodium bicarbonate is 3%, water is 93.65%.

[0033] The nutrient for the immobilized aerobic microorganisms of this embodiment can be obtained by uniformly mixing the above-mentioned raw material components.

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Abstract

The invention discloses a nutrient for immobilized aerobic microorganisms, which is composed of the following components according to weight percentage: 0.1%-0.3% of glucose, 0.5%-5% of ammonium chloride, and 0.05%-0.1% of magnesium sulfate , Sodium chloride is 0.05%~0.1%, Calcium chloride is 0.01%~0.1%, Potassium dihydrogen phosphate is 0.005%~0.05%, Potassium chloride is 0.005%~0.05%, Ferric sulfate is 0.001%~0.01% , sodium bicarbonate is 1 to 10%, and the balance is water. The invention also discloses a continuous culture method and continuous culture device for immobilized aerobic microorganism particles. The invention can promote rapid reproduction of aerobic microorganisms in immobilized particles, increase the density of microorganisms in unit volume, and accelerate the environmental adaptation process of aerobic microorganisms. The activity of immobilized aerobic microorganisms can be recovered in a short time, and the treatment effect is good and the cost is low.

Description

technical field [0001] The invention relates to a substrate, a method and a special device for continuous domestication, culture and enrichment of immobilized microbial products, which are applied in the technical field of microbial immobilization. Background technique [0002] With the aggravation of water pollution, nitrogen pollution in natural water bodies is serious. Aerobic microorganisms, especially nitrifying bacteria, are of great significance for the cycle conversion of nitrogen in nature. They can oxidize ammonia nitrogen into nitrite nitrogen or nitrate nitrogen, and then Nitrification removal plays a very important role in the removal of nitrogen pollution in water bodies. However, because aerobic microorganisms are troublesome autotrophic bacteria, they grow slowly and are sensitive to changes in environmental factors, so they are easily eliminated by the treatment system. Microbial immobilization technology can improve the concentration of microorganisms in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/00C12M1/00C12M1/04C12M1/38
Inventor 董亚梅王婷金彩虹何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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