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Preparation method and application of effective part of dioscorea persimilis for decreasing blood sugar

An effective part, the technology of yam yam, which is applied in the preparation and application field of the effective part of yam yam for lowering blood sugar, can solve the problems of insufficient hypoglycemic effect, inconvenient taking, and high cost, and achieves clear ED50, less environmental pollution, and low cost effect

Inactive Publication Date: 2014-01-22
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hypoglycemic effect of traditional Chinese medicine is not as strong as that of the above-mentioned modern drugs [4,5] , and most of them are compound prescriptions, with large dosage, high cost and inconvenient administration

Method used

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  • Preparation method and application of effective part of dioscorea persimilis for decreasing blood sugar
  • Preparation method and application of effective part of dioscorea persimilis for decreasing blood sugar
  • Preparation method and application of effective part of dioscorea persimilis for decreasing blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Carry out under the condition of room temperature 27 ℃-32 ℃, normal pressure. Take 5300g of dried coarse powder of yam rhizome, infiltrate it with 5300ml of edible ethanol, put it into the percolation cylinder, extract it according to the conventional percolation method, and collect the total percolation liquid of 53000ml (10 times the weight of the material in ml / g). Combine the percolation solution, recover ethanol under reduced pressure to a thick paste, then put it in a water bath and evaporate to dryness into a semi-solid extract (185.5g); add an equal amount of edible starch (185.5g) and mix with the semi-solid extract Granular dry extract; divide the dry extract into 4 equal parts, place them in four 250ml Erlenmeyer flasks with stoppers, add 150ml chloroform each, extract by ultrasonic for 15min, filter with medium-speed filter paper, discard the filtrate, collect Residue, the residue was extracted with chloroform according to the same method as above, and the o...

Embodiment 2

[0031] It is confirmed that the effective fraction prepared in Example 1 has the pharmacological effect of lowering blood sugar. Kunming hybrid mice (body weight 22-32g) received iv alloxan 70mg / kg once, were fed normally for 72 hours, and then blood was collected by retroocular venous plexus (fasting for 12 hours before blood collection), and blood glucose was measured. Animals with a blood sugar content ≥ 10 mmol / L (180 mg / dL) were selected as "hyperglycemia model mice" for hypoglycemic experiments. The modeling success rate was 71%.

[0032]The hyperglycemia model mice were randomly divided into 5 groups according to the blood glucose level and sex, and the analysis of variance showed that there was no significant difference in the mean blood glucose level among the groups (P>0.05). Two model groups were set up, and only the same amount of normal saline (ig) was administered, as well as the low-dose administration group (the effective part prepared in ig Example 1 was 200 ...

Embodiment 3

[0039] Determination of the hypoglycemic half effective dose ED of the hypoglycemic effective fraction prepared in Example 1 50 , quantitatively describe its hypoglycemic effect.

[0040] Kunming hybrid mice (body weight 22-32g) were treated with iv alloxan 65mg / kg once, fed normally for 72 hours, and then blood was collected by retroocular venous plexus (fasting for 12 hours before blood collection), and blood glucose was measured. Animals with a blood sugar content ≥ 10 mmol / L (180 mg / dL) were selected as "hyperglycemia model mice" for hypoglycemic experiments. The modeling success rate was 75%.

[0041] The hyperglycemia model mice were randomly divided into 5 groups according to the blood glucose level and sex, and the analysis of variance showed that there was no significant difference in the mean blood glucose level among the groups (P>0.05). Set up 5 administration groups with different doses, ig the effective part prepared in Example 1, and the dose ratio between gro...

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Abstract

The invention relates to a preparation method of an effective part of dioscorea persimilis for decreasing blood sugar and an application of the effective part. The preparation method of the effective part of dioscorea persimilis for decreasing blood sugar is characterized in that ethanol is adopted for extraction, chloroform and ethyl acetate are successively adopted for separating and removing impurities, a solvent is recycled and removed, thus the effective part in a form of thick paste or dry paste is obtained; conditions of a preparation technology comprise that temperatures in extraction and separation processes are normal temperatures in a production workshop and laboratory and pressure is normal pressure. The invention also relates to an application of the effective part of dioscorea persimilis for decreasing blood sugar and preparations of the effective part in prevention and treatment of diabetes. The preparation method of the effective part of dioscorea persimilis for decreasing blood sugar has the advantages that raw materials are easily available; pharmacological effects of decreasing blood sugar of the effective part are definite; the effective part is extract of a medicinal and edible traditional Chinese medicine, and safety is high; potency in decrease of blood sugar is definitely described in ED50 (50% effective dose); a preparation technology is simple, equipment requirements are low, technical risk is low, cost is low, and environmental pollution is hardly caused, so that the preparation method of the effective part of dioscorea persimilis for decreasing blood sugar is an efficient path for increasing additional value of dioscorea persimilis and expanding application range of dioscorea persimilis.

Description

technical field [0001] The invention relates to a preparation method (extraction and separation) of the effective part of yam yam for lowering blood sugar and the application of the effective part. Background technique [0002] Diabetes is one of the major diseases threatening human beings. There are 113.9 million adults with diabetes in my country and 493.4 million people with pre-diabetes. my country is the country with the largest number of diabetic patients in the world. [1,2] . [0003] Although there are many modern drugs with definite curative effects, such as insulin, sulfonylurea, and biguanide, their adverse reactions not only limit their use, but also bring certain risks. [3] . Therefore, searching for safer and more effective hypoglycemic drugs from traditional Chinese medicine has become a current hot spot. However, the hypoglycemic effect of traditional Chinese medicine is not as strong as that of the above-mentioned modern drugs [4,5] , and most of them a...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61P3/10A61K125/00
Inventor 何报作覃俊佳周芳
Owner GUANGXI UNIV OF CHINESE MEDICINE
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