Classic swine fever virus (CSFV) C strain E2 truncated protein and its preparation method and use

A technology of swine fever virus and truncated protein, which is applied in the direction of biochemical equipment and methods, viruses, virus peptides, etc., and can solve problems such as artificiality and uncertainty
CN103588864AActive Publication Date: 2014-02-19SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2014-02-19

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Abstract

The invention discloses a classic swine fever virus (CSFV) C strain E2 truncated protein and its preparation method and use. The main antigen region of the E2 gene is expressed and three pairs of disulfide bonds for maintaining the antigen space structure are expressed simultaneously so that the expressed protein has a certain space structure and satisfies natural protein characteristics and the inclusion body is conducive to protein purification. The E2 truncated protein obtained by the invention is used for building a CSFV IgG antibody ELISA detection method. A result shows that the recombinant protein has good antigenicity. The built ELISA kit adopting the CSFV C strain E2 truncated protein has good specificity, accuracy, sensitivity and repeatability after use. A substrate coloration solution used in the invention has an indication effect, and after several hours to 4 nights after the reaction, the detection result still has credibility. Therefore, the coloration system can reduced human factor influences, avoids false positive appearance, makes a negative result stable and guarantees good detection on CSFV antibodies.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a truncated protein of swine fever virus C strain E2, a preparation method and application thereof. Background technique

[0002] Classical Swine Fever (CSF) is an important viral infectious disease that seriously endangers the pig industry. The disease is prevalent worldwide. It is a major severe infectious disease of Class A stipulated by OIE, and its pathogen is also listed in the International Biological Weapons Convention. Listed as an important animal biological warfare agent or animal bioterrorism pathogen, it is highly valued by the international community.

[0003] Swine fever is an acute, febrile, highly contagious infectious disease of pigs caused by swine fever virus, also known as swine cholera. Classic swine fever virus (CSFV, formerly known as Hog cholera virus, HCV) is now a member of Flaviviridae and Pestivirus, members of the same genus also include bov...

Claims

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