A kind of production method of tea caterpillar nuclear polyhedrosis virus

A technology of nuclear polyhedrosis and production method, which is applied in the production field of tea caterpillar nuclear polyhedrosis virus, can solve the problems of affecting the feeding amount of larvae, death of overwintering eggs, and easily broken virus carcasses, so as to reduce the infection of tea caterpillar virus The probability of hair growth, the effect of ensuring the amount of virus production, and ensuring the amount of larva rearing

Active Publication Date: 2015-08-19
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, the virus yield is not high
Because tea caterpillar egg masses survive the winter for as long as 4-5 months, the leaves of hydroponic tea branches often fall and wither, resulting in the death of a large number of overwintering eggs, and the hatching rate of eggs is lower than 15%, which affects the feeding amount of the first generation larvae
At the same time, in the process of virus production and collection of dead insects, because the virus corpses are easily broken, there will be more losses during collection, and the virus yield per unit area will be low, thus affecting the virus production.

Method used

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  • A kind of production method of tea caterpillar nuclear polyhedrosis virus

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Embodiment 1

[0019] Put the potted tea tree into an insect cage (length 55cm×width 55cm×height 90cm, plastic gauze), insert 100-150 pairs of adult tea caterpillars, let them mate in the cage, and lay eggs on the back of the leaves. The insect cages are placed outdoors or indoors at natural temperature for overwintering.

[0020] After the eggs hatch, cut off the leaves with worms and put them in canning jars. One egg mass per bottle is added to several pieces of old leaves for feeding, and the mouth is tied with paper, and the cover glass is used to keep moisture. When most of the leaves in the bottle are eaten, remove the debris in time and add new leaves. When the larvae grow to the 4th instar, move the larvae in the bottle to a wooden box, about 1000-1500 worms per box, add tea branches with old leaves to raise, and cover the box with a wet towel and glass to moisturize.

[0021] When the larvae grow to the middle stage of the 6th instar, the poisoning treatment is carried out. The m...

Embodiment 2

[0025] After the adults start to emerge, put the canned bottle containing the tea caterpillar pupae into the insect cage (30cm×46cm×55cm, plastic gauze), open the paper cover of the bottle mouth, 3 bottles per cage, and place two in the cage at the same time. Bundle the tea branches cultivated in water, let the adults mate and lay eggs.

[0026] After the eggs hatch, cut off the leaves with worms and put them in canning jars. One egg mass per bottle is added to several pieces of old leaves for feeding, and the mouth is tied with paper, and the cover glass is used to keep moisture. When most of the leaves in the bottle are eaten, remove the debris in time and add new leaves. When the larvae grow to the 4th instar, move the larvae in the bottle to a wooden box, about 1000-1500 worms per box, add tea branches with old leaves to raise, and cover the box with a wet towel and glass to moisturize.

[0027] When the larvae grow to the middle stage of the 6th instar, the poisoning tr...

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Abstract

The invention discloses a method for producing tea caterpillar nuclear polyhedrosis virus, which belongs to the technical field of microbial pesticide production. It includes the following process steps: 1) Mass rearing of larvae; 2) Virus multiplication; 3) Virus collection; 4) Virus extraction. The present invention has the following beneficial effects: one is to prevent the skin allergy caused by the poisonous hairs of tea caterpillars, the present invention adopts the method of changing feed and collecting insect corpses in a fume hood, which greatly reduces the probability of being contaminated with poisonous hairs of tea caterpillars and effectively prevents allergies; Virus yield, the present invention adopts potted tea tree to produce overwintering eggs, significantly improved the hatching rate of overwintering eggs, and the hatching rate is raised to 65-80% from 10-15%, has ensured the 1st generation larva feeding amount and virus production amount in the coming year, simultaneously The invention adopts the virus mummy collection method, reduces the loss of the virus in the collection, improves the efficiency by 52.7% and the virus output by 38.8% compared with the conventional collection method.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticide production, and in particular relates to a production method of tea caterpillar nuclear polyhedrosis virus. Background technique [0002] tea caterpillar ( Euproctis pseudoconspersa ) is the main leaf-eating pest in my country's tea gardens. It not only affects the yield of tea, but also easily causes human skin allergies due to the poisonous hairs on the insects, seriously affecting the field management of tea gardens. Tea caterpillar nuclear polyhedrosis virus ( Euproctis pseudoconspersa nucleopolyhedrovirus (EpNPV for short) is a dominant species of natural enemies of tea caterpillars, which has the advantages of good insecticidal effect on tea caterpillars, natural enemies of tea gardens and environmental safety. The production of tea caterpillar nuclear polyhedrosis virus adopts live worm proliferation method, and its production process is as follows: firstly, a large number of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 唐美君殷坤山郭华伟肖强
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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