A circulating tumor cell capture instrument and its capture staining method

Abstract

The invention discloses a capture instrument for circulating tumor cells. The capture instrument comprises a multi-way valve device, a wheel disc device, a sample loading needle device and an injector device, wherein the multi-way valve device is connected with a reagent bag, different kinds of reagents can be selected through the multi-way valve device, the wheel disc device is provided with a filter, the sample loading needle device can be inserted into the filter of the wheel disc device so as to be communicated with an air path and a liquid path, the injector device absorbs the required reagent through the multi-way valve device and then injects the reagent into the filter. Furthermore, the invention also discloses a method for capturing and staining by using the capture instrument for circulating tumor cells. The retention rate of the capture instrument in capturing tumor cells is higher than that of manual capturing; furthermore, compared with manual staining, the capture instrument for circulating tumor cells has high degree of uniformity and consistency in staining, and is clean in background.

Description

technical field [0001] The invention relates to a biological detection instrument, in particular to a circulating tumor cell capture instrument and a capture staining method. Background technique [0002] Tumor micrometastases originate from invading circulating tumor cells. Therefore, circulating tumor cells (circulating tumor cells, CTCs) may be a sign of distant metastasis of tumors. In recent years, with the development of sensitive molecular techniques, it has become possible to separate and count peripheral blood CTCs. In the early stage of the disease, CTCs may help to differentiate benign and malignant tumors, and predict and judge the risk of metastasis; in the advanced stage of the disease, CTCs may provide important prognostic information and help doctors monitor the effect of treatment; and CTCs may reflect tumor The characteristics can guide the formulation of treatment plan and can be used as the target of targeted therapy. There are many methods for the sepa...

AI Technical Summary

Problems solved by technology

Gradient centrifugation and filtration are also important means and methods for CTCs cell capture. FrankA.W.Coumans and others have achieved good results in the laboratory with filtration. Regarding the use of membrane filtration to capture CTCs cells, scientists and Scholars have conducted extensive research, but most of them use laboratory methods and manual operations. The second phase of implementation has a single throughput. The manual operation process has brought huge problems to industrialization. It is the manual operation process that will introduce corresponding operating errors, which will affect the experimental results, and human factors will have a greater impact. So far, there is no commercial product that uses this method on the market.

Images