Reciprocating type circulating single micro-channel device facing fluorescent PCR (Polymerase Chain Reaction) micro-system
A channel device and micro-system technology, applied in the field of reciprocating cycle single micro-channel device, can solve the problems of not ensuring the complete reaction of reagents, difficulty in controlling temperature accuracy, restricting PCR, etc. The effect of surface roughness
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[0018] Attached below Figure 1~2 Describe this embodiment in detail:
[0019] The device general structure diagram of the present invention is as figure 1 and the working schematic diagram of the device is shown in figure 2 As shown, the principle that PCR reagents are immiscible in air or silicone oil is mainly used to design a single-cycle structural module to realize the continuous reaction of reagents in multi-temperature zones. Using a metal tube microchannel as the main body of the device, fill the metal tube microchannel with silicone oil, and then add PCR reagent 11 into it; since the reagent is not compatible with silicone oil, a PCR reagent segment will appear in the ring microchannel, And the metal tube microchannel is divided into three different temperature zones: annealing low temperature zone 3, extended temperature zone 4, thermal variable high temperature zone 5, and the power device is used to complete 40 cycles of the PCR reagent section in the three tem...
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