Reciprocating type circulating single micro-channel device facing fluorescent PCR (Polymerase Chain Reaction) micro-system

A channel device and micro-system technology, applied in the field of reciprocating cycle single micro-channel device, can solve the problems of not ensuring the complete reaction of reagents, difficulty in controlling temperature accuracy, restricting PCR, etc. The effect of surface roughness

Inactive Publication Date: 2014-03-05
BEIJING UNIV OF TECH
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Problems solved by technology

Due to the constraints of the current heating and refrigeration technology, micro heating and refrigeration devices have speed limits for cooling and heating, which restricts the time of PCR amplification; in addition, it is also difficult to control the temperature accuracy, and it cannot guarantee that the reagents are completely reacted or in Other reactions occur during temperature changes, causing errors

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  • Reciprocating type circulating single micro-channel device facing fluorescent PCR (Polymerase Chain Reaction) micro-system
  • Reciprocating type circulating single micro-channel device facing fluorescent PCR (Polymerase Chain Reaction) micro-system

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Embodiment Construction

[0018] Attached below Figure 1~2 Describe this embodiment in detail:

[0019] The device general structure diagram of the present invention is as figure 1 and the working schematic diagram of the device is shown in figure 2 As shown, the principle that PCR reagents are immiscible in air or silicone oil is mainly used to design a single-cycle structural module to realize the continuous reaction of reagents in multi-temperature zones. Using a metal tube microchannel as the main body of the device, fill the metal tube microchannel with silicone oil, and then add PCR reagent 11 into it; since the reagent is not compatible with silicone oil, a PCR reagent segment will appear in the ring microchannel, And the metal tube microchannel is divided into three different temperature zones: annealing low temperature zone 3, extended temperature zone 4, thermal variable high temperature zone 5, and the power device is used to complete 40 cycles of the PCR reagent section in the three tem...

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Abstract

The invention relates to a reciprocating type circulating single micro-channel device facing a fluorescent PCR (Polymerase Chain Reaction) micro-system and belongs to the detection fields of biology, analytical chemistry and medical science. The reciprocating type circulating single micro-channel device is characterized in that three temperature regions in PCR amplification are composed of metal pipes with good thermal performances; the three metal pipe temperature regions are connected by using ultraviolet laser cure optical colloids; the reciprocating type circulating single micro-channel device not only has the effects of isolating heat, sealing and supporting, but also has the light transmitting property; a mini-type fluorescent detection system can also be arranged to form a real-time fluorescent PCR microfluidic system. The reciprocating type circulating single micro-channel device has the essences that compressed air or silicone oil moves back and forth in a single micro-channel and a small section of a PCR reagent is used for finishing the PCR amplification of forty temperature cycles in the three temperature regions. Therefore, the volume and the weight are reduced, the reagent is saved and the reciprocating type circulating single micro-channel device is suitable for automatically working in a space on-orbit zero-gravity environment.

Description

technical field [0001] The invention relates to a reciprocating cycle single microchannel device for fluorescent PCR microsystems, which is mainly used for subsequent construction of PCR microstructure reactions and PCR reaction real-time fluorescence detection devices applied within a spatial range, and belongs to biology, analytical chemistry and field of medical testing. Background technique [0002] There are harmful microorganisms in the spacecraft that threaten the health of astronauts and corrode materials. There is an urgent need for a real-time automatic alarm system for space on-orbit biohazards. The core is a microbial nucleic acid fluorescence detection microsystem. Due to the space on-orbit, the equipment is required to have the technical characteristics of full automation, small volume and small weight. Therefore, simplifying the microfluidic fluorescent PCR working system is the key to realize the real-time automatic alarm system requirements for space on-orb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34
CPCB01L3/502707B01L3/50273B01L7/525B01L2200/0636B01L2200/10B01L2200/12B01L2300/087B01L2300/18B01L2400/0478
Inventor 吴坚于伦王富吉陈涛郑杨
Owner BEIJING UNIV OF TECH
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