Method capable of quantitatively detecting surface enhanced Raman spectroscopy (SERS) of polychlorinated biphenyl

A surface-enhanced Raman and polychlorinated biphenyl technology is applied in Raman scattering, preparation of test samples, material excitation analysis, etc., to achieve good uniformity in shape and particle size

Active Publication Date: 2014-03-05
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

However, the use of SERS for quantitative measurement is sti

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  • Method capable of quantitatively detecting surface enhanced Raman spectroscopy (SERS) of polychlorinated biphenyl
  • Method capable of quantitatively detecting surface enhanced Raman spectroscopy (SERS) of polychlorinated biphenyl
  • Method capable of quantitatively detecting surface enhanced Raman spectroscopy (SERS) of polychlorinated biphenyl

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Embodiment 1

[0021] Substrate preparation and characterization of gold-coated silica materials

[0022] First, to synthesize 1-3nm gold seeds, add 0.5mL of 1.0M sodium hydroxide to 45mL of ultrapure water, followed by adding 50mM of THPC. Here, THPC acts as both a reducing agent and a surfactant. After the mixed solution was vigorously stirred for 5 min, 36 μL of 1.0 M chloroauric acid solution was added. The color of the reaction solution changed from colorless to dark brown immediately, the stirring speed was reduced, and the stirring was continued for 15 minutes. The obtained reaction solution was stored in a refrigerator at 4°C for future use. Then, APTMS-modified silica nanoparticles were synthesized. For the preparation of silica nanospheres, first mix 3mL of ammonia solution (30wt%) with 50mL of pure alcohol solution, then add 1.5mL of TEOS solution, and stir the mixed solution overnight at low speed. Take 10 mL milky white product and add excess APTMS (200 μL), and the mixture ...

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Abstract

The invention discloses a method capable of quantitatively detecting surface enhanced Raman spectroscopy (SERS) of polychlorinated biphenyl. The method comprises the following steps: preparing uniform gold-coated silica nanoparticles, and fixing nanoparticles in a core-shell structure on a quartz glass piece to form an SERS substrate by utilizing a coupling agent; connecting an aptamer of a printed circuit board (PCB) with a thiol at one end, and covalently connecting the aptamer of the PCB to the surface of a gold shell layer of the nanoparticles through a thiol; soaking the prepared SERS substrate in a PCB-containing solution, taking the SERS substrate out after sufficient time, cleaning by using deionized water, and performing Raman spectrum measurement. The prepared gold-coated silica nanoparticles are attractive in appearance and high in particle size uniformity, plasma resonance occurs at 750nm, and a strong SERS enhancement effect is achieved under excitation of 785nm<-1> laser.

Description

technical field [0001] The content of the present invention mainly includes the design and preparation of gold-coated silica core-shell nanoparticles connected with PCB aptamer single-stranded DNA, using the change characteristics of the surface-enhanced Raman scattering spectrum of its aptamer single-stranded DNA, Realize specific and quantitative measurement and analysis of PCB. Background technique [0002] Polychlorinated biphenyls (PCB: Polychlorinated biphenyls) are organic substances that are not easily decomposed in the environment. Most of them are toxic and will accumulate in organisms, causing environmental pollution and endangering the survival of animals and humans. For monitoring and quantitative analysis of PCBs, traditional methods include gas chromatography, liquid chromatography, liquid chromatography-mass spectrometry, and other methods. Spectral methods have also attracted people's attention, such as phosphorescence and fluorescence methods for PCB de...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28
Inventor 黄青鲁逸林
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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