33results about How to "Enhanced Raman scattering effect" patented technology

The invention discloses a method capable of quantitatively detecting surface enhanced Raman spectroscopy (SERS) of polychlorinated biphenyl. The method comprises the following steps: preparing uniform gold-coated silica nanoparticles, and fixing nanoparticles in a core-shell structure on a quartz glass piece to form an SERS substrate by utilizing a coupling agent; connecting an aptamer of a printed circuit board (PCB) with a thiol at one end, and covalently connecting the aptamer of the PCB to the surface of a gold shell layer of the nanoparticles through a thiol; soaking the prepared SERS substrate in a PCB-containing solution, taking the SERS substrate out after sufficient time, cleaning by using deionized water, and performing Raman spectrum measurement. The prepared gold-coated silica nanoparticles are attractive in appearance and high in particle size uniformity, plasma resonance occurs at 750nm, and a strong SERS enhancement effect is achieved under excitation of 785nm<-1> laser.

The invention discloses an optical fiber type sensor system based on surface plasma resonance and stimulated Raman scattering. The system comprises a broadband light source, a P-type polaroid, a focusing convex lens, a polarization controller, an optical fiber beam splitter, an optical fiber inclined end, a surface plasma resonance (SPR) probe and an optical fiber spectrometer, wherein the P-type polaroid is positioned between the broadband light source and the focusing convex lens; the focusing convex lens optically couples the polarization broadband to the polarization controller; the polarization controller is connected with one input end of the optical fiber beam splitter, and the optical fiber spectrometer is connected with the other input end of the optical fiber beam splitter; the optical fiber inclined end is connected with one output end of the optical fiber beam splitter, and the SPR probe is connected with the other output end of the optical fiber beam splitter; the free end of the optical fiber inclined end is inclined; the SPR probe comprises an optical fiber; the end face of an exposed fiber core is provide with a first metal film, and the periphery of the exposed fiber core is provide with a second metal film; a nano-film is arranged on the surface of the second metal film; and a sensitive film is arranged on the nano-film. The system has the advantages of high universality, sensitivity and stability, and capable of realizing remote monitoring.

The invention discloses an optical fiber probe enhanced type portable Raman spectrometer. The optical fiber probe enhanced type portable Raman spectrometer comprises a Raman spectrometer main unit, an optical fiber Raman probe and an optical fiber SERS (surface enhanced Raman scattering) probe, wherein a laser, a detector and a switching power supply are arranged in the Raman spectrometer main unit, and an FC/PC optical fiber interface directly connected with the laser and an SMA905 optical fiber interface directly connected with the detector are reserved on a front panel of the Raman spectrometer main unit; the optical fiber Raman probe comprises an optical coupling module, an input optical fiber, an output optical fiber and a Raman detection end; one end of the input optical fiber and one end of the output optical fiber are connected with the two optical fiber interfaces of the Raman spectrometer main unit respectively; the other ends of the input optical fiber and the output optical fiber are jointly connected into the optical coupling module, and the optical coupling module is connected with the Raman detection end simultaneously; the Raman detection end is in direct coupling connection with the optical fiber patch cord end of the optical fiber SERS probe. The optical fiber probe enhanced type portable Raman spectrometer has the advantages of being miniaturized, high in flexibility, good in repeatability, simple in detection process and the like.

The invention provides a surface enhanced raman spectrum based quick tetrodotoxin (TTX)detection method of tetrodotoxin (TTX). A nano Fe3O4 particle is adopted as a magnetic substrate; Fe3O4 is modified in sequence by using tetraethoxysilane (TEOS), 3-aminopropyltriethoxysilane and 3-mercaptopropyltriethoxysilane in sequence; a gold nano particle is adsorbed on a modified magnetic nano particle through the electrostatic adsorption of -NH2 and the action of a Au-S bond; a well assembled gold magnetic nano particle is integrally modified by using the TEOS and the 3-aminopropyltriethoxysilane; an immunocompetent gold magnetic nano particle is used as a raman substrate; RhB-ITC (Rhodamine B-Isothiocyanate)/anti-TTX is used as a raman signaling molecule; the quick detection is carried out on the TTX by utilizing a surface enhanced raman spectrometer; the detection limit can reach 0.01[mu]g/ml in under the condition that the gold magnetic nano particle is concentrated by an outer magnet; the detection linear range is 0.01[mu]g/mL to 0.5[mu]g/mL; the recovery rate of a detected sample is 82.64 to 92.60 percent; the relative standard deviation is 2.6 to 11.1 percent. The detection of the TTX has important significance in the aspects of food safety and medical science.

The invention relates to a preparation method of a substrate material with a surface enhanced Raman scattering effect. The preparation method comprises the steps of preparing dispersion liquid of carboxyl polystyrene microspheres with negative charges on surfaces; uniformly mixing the dispersion liquid of the carboxyl polystyrene microspheres with negative charges on surfaces and a stannous chloride solution, so as to obtain dispersion liquid of tin polystyrene microspheres; adding a silver-ammonia solution and a reductant into the dispersion liquid of the tin polystyrene microspheres in sequence to obtain mixed liquor; enabling the mixed liquor to react under 20 to 40 DEG C for 0.5 to 2 hours, thus generating the substrate material with the surface enhanced Raman scattering effect. According to the preparation method of the substrate material with the surface enhanced Raman scattering effect, irregular gathering of sol-state metal nanoparticles can be avoided, reaction conditions are mild, requirements on equipment are low, and the process is simple.

The invention relates to a preparation method of polydopamine coated side-by-side gold nanorod self-assembly composite nanostructures and products. The method uses isopropanol and polyacrylic acid forinducing side-by-side self assembly of gold nanorods, and uses polydopamine for coating gold nanorod self-assembly structures; and the obtained gold nanorods are axially arranged and assembled in sequence to form the gold nanorod side-by-side self-assembly structures. The preparation method is simple in operation, good in repeatability and low in cost; and the obtained polydopamine coated side-by-side gold nanorod self-assembly composite nanostructures achieve better plasma resonance optical properties, show excellent photothermal effect and surface-enhanced Raman scattering effect, are excellent in photothermal conversion efficiency and self heat resistance, and achieve better application prospect in the fields of nanometer photothermal diagnosis and treatment agents and optical sensing.

The invention relates to the technical field of hepatitis C virus diagnosis, and discloses a saliva diagnostic sensor as well as a preparation method thereof and application thereof in detection of hepatitis C. The sensor comprises a substrate, a metal film and composite nano rolls, wherein the metal film covers the substrate; the composite nano rolls are dispersed on the metal film; the compositenano rolls respectively have a nano roll structure which is formed by enabling metal nanoparticles to grow on the surface of a graphene material in situ, then rolling up the graphene material and enabling the metal nanoparticles to be wrapped in the graphene material. The preparation method of the sensor comprises the steps of enabling a liquid phase reaction system containing the graphene material, metal ions and an ethanol solution to be subjected to an ultrasonic reaction to prepare the composite nano rolls; coating the substrate, with the sputtered metal film, with dispersion liquid of the composite nano rolls, and drying to obtain the saliva diagnostic sensor. Compared with the prior art, the saliva diagnostic sensor has the advantages of simple operation, rapid detection speed and high accuracy.

The invention relates to a surface-enhanced Raman scattering substrate based on a metal nanobowl and a preparation method thereof. The surface-enhanced Raman scattering substrate based on the metal nanobowl is composed of a polymer nanobowl and metal films, and the metal films cover the inner surface and the outer surface of the polymer nanobowl; the enhancement factor of the surface-enhanced Raman scattering substrate based on the metal nanobowl is 107 to 109; the enhancement factor is the ratio of Raman signal intensity of dye solutions with the same concentration on the enhanced substrate and a non-enhanced substrate; the enhanced substrate is a surface-enhanced Raman scattering substrate based on the metal nanobowl, and the difference between the non-enhanced substrate and the enhancedsubstrate is that the non-enhanced substrate does not have a metal film. The preparation method comprises the steps of taking the polymer nanobowl as a substrate, and vertically sputtering a metal film on the substrate to prepare the surface-enhanced Raman scattering substrate based on the metal nanobowl. The preparation method disclosed by the invention is simple, and the prepared surface-enhanced Raman scattering substrate based on the metal nanobowl is remarkable in enhancement effect.

The invention discloses an application of mixed crystalline semiconductor nanoparticles as a substrate for surface enhanced laser Raman spectroscopy. According to the application of the mixed crystalline semiconductor nanoparticles as the substrate of the surface-enhanced laser Raman spectroscopy, the mixed crystalline semiconductor nanoparticles are selected from metal oxides with a crystal and non-crystal combined structure. The mixed crystalline semiconductor nanoparticles not only have a high-selectivity surface enhanced Raman scattering effect, but also have higher surface enhanced Raman sensitivity than semiconductor crystals, and are beneficial to improving the biological detection sensitivity.

The invention discloses a preparation method for a biological detection chip for silver-porous silicon-based surface enhanced Raman scattering. The preparation method for the biological detection chipfor silver-porous silicon-based surface enhanced Raman scattering comprises the following steps of S10, taking a silicon wafer as an anode and a platinum sheet as a cathode, and placing the anode andthe cathode in an electrolyte for anode corrosion reaction in order to obtain a multilayer Bragg porous silicon substrate; and S20, placing the multilayer Bragg porous silicon substrate in a silver nitrate solution for immersion treatment, taking out and drying, and performing heat treatment to obtain the biological detection chip for silver-porous silicon-based surface enhanced Raman scattering.According to the preparation method, porous silicon is used as a photonic crystal and silver particles are used as a medium for enhancing an electromagnetic field, the method has the characteristicsof high amplification factor, stable property, long-term use, wide application range, easiness in operation and smaller human operation error, a Raman signal can be effectively amplified to millions of times, the interference of fluorescence is reduced, and the shelf life and repeatability are greatly improved.

The invention relates to an SERS (Surface Enhanced Raman Scattering) substrate with a surface-enhanced Raman scattering effect, in particular to a three-dimensional liquid in-situ SERS detection substrate as well as a preparation method and application thereof. Silver and gold salt solutions are sequentially introduced into a channel tube packaged with a metal wire, a dendritic gold-silver composite multilevel structure penetrating through the metal wire and the channel tube is prepared through a replacement reaction, the three-dimensional liquid in-situ SERS detection substrate is formed, and the problems that an existing SERS substrate is difficult to make full contact with flowing liquid, detection signal sensitivity is low, repeatability is poor and the like are solved. The SERS substrate is embedded in the channel pipe, and liquid to be detected flows through the channel pipe, so that in-situ real-time detection of the detected liquid is realized.