Preparation method for biological detection chip for silver-porous silicon-based surface enhanced Raman scattering

A surface-enhanced Raman, porous silicon-based technology, applied in Raman scattering, nanotechnology for materials and surface science, measurement devices, etc., can solve the problems of restricting wide application, complicated preparation process, short shelf life, etc., to achieve Highly enhanced Raman signal capability, good repeatability and shelf life, and stable properties

Active Publication Date: 2020-07-03
新疆艾旗斯德检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are many surface modification methods for porous silicon: non-metallic surface modification, noble metal surface modification, transition metal surface modification, semiconductor composite modification, etc., the preparation process is complicated, and the repeatability is poor, and the shelf life is short, which greatly limits the extensive use of silicon. application

Method used

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  • Preparation method for biological detection chip for silver-porous silicon-based surface enhanced Raman scattering
  • Preparation method for biological detection chip for silver-porous silicon-based surface enhanced Raman scattering
  • Preparation method for biological detection chip for silver-porous silicon-based surface enhanced Raman scattering

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Experimental program
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Effect test

Embodiment 1

[0061] The specific operation steps are as follows:

[0062] (1) N-type silicon wafer pretreatment: first boil 10min with mixed solution 1, then boil with mixed solution 2 for 10min, and then use CH with a mass fraction of 99.5% 3 COCH 3 Ultrasonic cleaning for 15 minutes, and finally ultrasonic cleaning with deionized water for 15 minutes.

[0063] Mixture 1 consists of H 2 O, H 2 o 2 and NH with a mass fraction of 28% 3 ·H 2 O is composed according to the volume ratio of 5:1:1;

[0064] Mixture 2 consists of H 2 O, H 2 o 2 and HCl with a mass fraction of 38% according to the volume ratio of 6:2:1.

[0065] (2) take the metal Pt sheet as the cathode, and the silicon sheet obtained by the pretreatment in the step (1) is the anode, and the distance between the two electrodes is 7cm, placed in the HF and C 3 h 5 OH in a mixed solution with a volume ratio of 1:1 for electrochemical anodic corrosion to obtain a multilayer Bragg porous silicon substrate.

[0066] Elect...

Embodiment 2

[0070] The application of the silver-porous silicon-based surface-enhanced Raman scattering biological detection chip prepared in Example 1 in the Raman signal amplification of rhodamine 6G, and the application of the Raman characteristic peak amplification in the serum of breast cancer patients, specifically Including the following steps:

[0071] Configure the concentration as 10 respectively -6 , 10 -7 , 10 -8 , 10 -9 , 10 -10 , 10 -11 , 10 -12 , 10 -13 Each mol / L rhodamine 6G solution was 50ml, and the silver-porous silicon-based surface-enhanced Raman scattering biological detection chip was put into the solution for 3 hours and then taken out. SERS spectra were obtained using a HORIBA Raman microspectrometer (HORIBA Jobin Yvon, Kyoto, Japan) equipped with a 50 mW Nd:YAG laser with 532 nm light and a He–Ne laser with 633 nm light as excitation radiation.

[0072] In order to avoid the sample heating effect, a low laser power of 2.5 mW was used, and the diameter of...

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Abstract

The invention discloses a preparation method for a biological detection chip for silver-porous silicon-based surface enhanced Raman scattering. The preparation method for the biological detection chipfor silver-porous silicon-based surface enhanced Raman scattering comprises the following steps of S10, taking a silicon wafer as an anode and a platinum sheet as a cathode, and placing the anode andthe cathode in an electrolyte for anode corrosion reaction in order to obtain a multilayer Bragg porous silicon substrate; and S20, placing the multilayer Bragg porous silicon substrate in a silver nitrate solution for immersion treatment, taking out and drying, and performing heat treatment to obtain the biological detection chip for silver-porous silicon-based surface enhanced Raman scattering.According to the preparation method, porous silicon is used as a photonic crystal and silver particles are used as a medium for enhancing an electromagnetic field, the method has the characteristicsof high amplification factor, stable property, long-term use, wide application range, easiness in operation and smaller human operation error, a Raman signal can be effectively amplified to millions of times, the interference of fluorescence is reduced, and the shelf life and repeatability are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biological signal detection materials, in particular to a method for preparing a silver-porous silicon-based surface-enhanced Raman scattering biological detection chip. Background technique [0002] The enhancement of the Raman signal is through the interaction between the noble metal nanoparticle substrate and the absorbed target molecules, resulting in a stronger Raman signal due to the localized surface plasmon resonance [LSPR] of these plasmonic nanostructures, namely Surface Enhanced Raman Scattering (SERS). SERS has two enhancement modes, one is chemical enhancement and the other is structural enhancement. Among them, one of the most effective methods for structural enhancement is the plasmonic coupling between adjacent nanoparticles, which leads to a significant increase in LSPR (i.e., "hot spot") in the interstitial region of adjacent nanostructures. Synthesizing regularly arranged silver nanop...

Claims

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Application Information

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IPC IPC(8): C25F3/12B22F9/24B22F1/00B22F1/02G01N21/65B82Y30/00B82Y40/00
CPCC25F3/12B22F9/24G01N21/658B82Y30/00B82Y40/00B22F2009/245B22F1/142B22F1/054B22F1/16
Inventor 侯军伟马小荣张元勃刘亚锋张志强陈晨严紫薇吕小毅陈紫荆惠泽友
Owner 新疆艾旗斯德检测科技有限公司
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