Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of gold magnetic nano particle and quick detection thereof on tetrodotoxin by combining with surface enhanced raman spectrometry

A technology of gold magnetic nanoparticles and tetrodotoxin, applied in Raman scattering, measurement devices, and material analysis through optical means, can solve problems such as unsuitable for rapid detection, poor repeatability, and low sensitivity

Active Publication Date: 2017-10-20
ZHEJIANG UNIV OF TECH
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the mouse biological method can accurately and stably detect tetrodotoxin, and is a legal method for determining TTX content in Japan, it is time-consuming and laborious, with poor repeatability and lack of specificity
However, liquid chromatography is mostly used for the quantitative detection of tetrodotoxin, but it requires complex and time-consuming pretreatment of samples before detection, which is not suitable for rapid on-site detection.
Thin-layer chromatography is simple to operate, but the sensitivity is too low for quantitative detection and analysis
The antiserum preparation method in the immunological method is called simple, but because the specificity of the monoclonal antibody is poor, there may be cross-reaction, so it will have a great impact on the quantitative detection of tetrodotoxin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of gold magnetic nano particle and quick detection thereof on tetrodotoxin by combining with surface enhanced raman spectrometry
  • Preparation of gold magnetic nano particle and quick detection thereof on tetrodotoxin by combining with surface enhanced raman spectrometry
  • Preparation of gold magnetic nano particle and quick detection thereof on tetrodotoxin by combining with surface enhanced raman spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] i) Surface Band-NH 2 and Fe of -SH 3 o 4 / SiO 2 Preparation of nanoparticles

[0050] Take 4.00mg Fe 3 o 4 Nanoparticles were washed twice with distilled water, collected by magnetic separation after washing, dispersed with 2mL distilled water, put into a 15mL glass bottle, and 11mL absolute ethanol and 0.2mL ammonia water were added at the same time. Sonicate for 10 minutes under the condition of pH=11, add 8 μL TEOS, and react under ultrasound for 8 hours. After the reaction, the particles are collected by magnetic separation, washed twice with absolute ethanol and distilled water, and finally mixed with 2 mL of distilled water and 11 mL of absolute ethanol. Disperse in the mixed liquid, add 0.2mL ammonia water, sonicate for 10min, then add 5.3μL APTES and 5.3μL MPTES, and react for 18h under the action of sonication. After the reaction, the particles were collected by magnetic separation to obtain the surface band -NH 2 and Fe of -SH 3 o 4 Nanoparticles were...

Embodiment 2

[0057] (1) Surface Tape-NH 2 and Fe of -SH 3 o 4 / SiO 2 Preparation of nanoparticles

[0058] Take 4.00mg Fe 3 o 4 Nanoparticles were washed twice with distilled water, collected by magnetic separation after washing, dispersed with 2mL distilled water, put into a 15mL glass bottle, and 9mL absolute ethanol and 0.2mL ammonia water were added at the same time. Sonicate for 5 minutes under the condition of pH = 10, add 8 μL TEOS, and react under ultrasound for 6 hours. After the reaction, the particles are collected by magnetic separation, washed twice with absolute ethanol and distilled water, and finally mixed with 2 mL of distilled water and 9 mL of absolute ethanol. Disperse in the mixture, add 0.2mL ammonia water, sonicate for 5min, then add 3.2μL APTES and 3.2μL MPTES, and react for 16h under the action of sonication. After the reaction, the particles were collected by magnetic separation to obtain the surface band -NH 2 and Fe of -SH 3 o4 Nanoparticles were washed ...

Embodiment 3

[0066] (A) Only modify -NH 2 Fe 3 o 4 / SiO 2 magnetic nanoparticles

[0067] Take 4.00mg Fe 3 o 4 Nanoparticles were washed twice with distilled water, collected by magnetic separation after washing, dispersed with 2mL distilled water, put into a 15mL glass bottle, and 10mL absolute ethanol and 0.2mL ammonia water were added at the same time. Sonicate for 5 minutes under the condition of pH = 10, add 8 μL TEOS, and react for 6 hours under ultrasound. After the reaction, the particles are collected by magnetic separation and magnetic separation, washed twice with absolute ethanol and distilled water, and finally dispersed with 2 mL and 10 mL of absolute ethanol , add 0.2mL ammonia water, sonicate for 5min, then add 8μL APTES, and react for 16h under the action of sonication. After the reaction, the particles were collected by magnetic separation to obtain the surface band -NH 2 Fe 3 o 4 Nanoparticles were washed twice with absolute ethanol and distilled water, and fina...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Volume usageaaaaaaaaaa
Login to View More

Abstract

The invention provides a surface enhanced raman spectrum based quick tetrodotoxin (TTX)detection method of tetrodotoxin (TTX). A nano Fe3O4 particle is adopted as a magnetic substrate; Fe3O4 is modified in sequence by using tetraethoxysilane (TEOS), 3-aminopropyltriethoxysilane and 3-mercaptopropyltriethoxysilane in sequence; a gold nano particle is adsorbed on a modified magnetic nano particle through the electrostatic adsorption of -NH2 and the action of a Au-S bond; a well assembled gold magnetic nano particle is integrally modified by using the TEOS and the 3-aminopropyltriethoxysilane; an immunocompetent gold magnetic nano particle is used as a raman substrate; RhB-ITC (Rhodamine B-Isothiocyanate) / anti-TTX is used as a raman signaling molecule; the quick detection is carried out on the TTX by utilizing a surface enhanced raman spectrometer; the detection limit can reach 0.01[mu]g / ml in under the condition that the gold magnetic nano particle is concentrated by an outer magnet; the detection linear range is 0.01[mu]g / mL to 0.5[mu]g / mL; the recovery rate of a detected sample is 82.64 to 92.60 percent; the relative standard deviation is 2.6 to 11.1 percent. The detection of the TTX has important significance in the aspects of food safety and medical science.

Description

technical field [0001] The invention relates to a preparation method of gold magnetic nanoparticles and its application in surface-enhanced Raman spectrum rapid detection of tetrodotoxin. Background technique [0002] Tetrodotoxin (TTX) is an alkaloid contained in puffer fish (commonly known as puffer fish) and other organisms. Its molecular formula is C 11 h 17 o 8 N 3 , with a molecular weight of 319. It is an amino perhydroquinazoline compound, one of the most toxic neurotoxins found in nature, and was once considered to be the most toxic non-protein toxin in nature. 0.5mg can cause death. The chemical and thermal properties of tetrodotoxin are very stable, and can only be decomposed by heating at high temperature for more than 30 minutes or under alkaline conditions. [0003] At present, the detection methods of tetrodotoxin mainly include mouse biological method, liquid chromatography, thin layer chromatography, immunological method and so on. Although the mouse...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 能静孙培龙项晨陆嘉晖王栩俊郏侃
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com