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Method for comprehensive extraction of effective constituents in mushrooms

A technology of active ingredients and shiitake mushrooms, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of low extraction rate, waste, no utilization of shiitake mushrooms, etc., achieve high extraction rate, increase dissolution, The effect of reducing waste

Active Publication Date: 2014-11-26
万洲胶粘制品(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the methods for extracting fatty acids mainly use petroleum ether or n-hexane using Soxhlet extractor, ultrasonic extraction or microwave extraction. The extraction of polysaccharides and amino acids mainly adopts water extraction. The current method only uses a single method for extraction, and the extraction rate is low. , and the current method does not comprehensively utilize the substances in the shiitake mushrooms, resulting in a certain degree of waste

Method used

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  • Method for comprehensive extraction of effective constituents in mushrooms
  • Method for comprehensive extraction of effective constituents in mushrooms

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for comprehensively obtaining active ingredients from shiitake mushrooms, comprising the steps of:

[0030] 1) Take the whole fruiting bodies of shiitake mushrooms, crush them in an electric mill, take 5.00g and treat them in a high-pressure steam sterilizer at a gauge pressure of 100KPa and 121°C for 1 hour;

[0031] 2) Add the product obtained in step (1) into n-hexane according to the mass ratio of mushroom fruiting body powder and n-hexane of 1:10, use a Soxhlet extractor to extract at 70°C for 8 hours, and filter to obtain fatty acid extraction liquid and the residue after extraction;

[0032] 3) Concentrating the fatty acid extract in a rotary evaporator under the conditions of 40°C and a gauge pressure of -0.09Mpa to obtain a fatty acid extract of Lentinus edodes;

[0033] The extracted residue was added to an aqueous solution of 1.2% papain at a mass concentration of 20 times by mass, ultrasonically treated at a power of 200W for 10 minutes, and then r...

Embodiment 2

[0042] A method for comprehensively obtaining active ingredients from shiitake mushrooms, comprising the steps of:

[0043] 1) Take the whole fruiting bodies of shiitake mushrooms, crush them in an electric mill, take 5.00g and treat them in a high-pressure steam sterilizer at a gauge pressure of 165KPa and 109°C for 2 hours;

[0044] 2) Add the product obtained in step (1) into petroleum ether according to the mass ratio of mushroom fruiting body powder and petroleum ether at a ratio of 1:15. In an ultrasonic cleaner, the extraction time is 2 hours at an ultrasonic frequency of 100 Hz. , filtered to obtain the fatty acid extract and the residue after extraction;

[0045] 3) Concentrating the fatty acid extract in a rotary evaporator under the conditions of 40°C and a gauge pressure of -0.09Mpa to obtain a fatty acid extract of Lentinus edodes;

[0046] The extracted residue was added to an aqueous solution of 2.0% papain at a mass concentration of 17 times by mass, ultrasonica...

Embodiment 3

[0054] A method for comprehensively obtaining active ingredients from shiitake mushrooms, comprising the steps of:

[0055] 1) Take the whole fruiting bodies of shiitake mushrooms, crush them in an electric mill, take 5.00g and treat them in a high-pressure steam sterilizer at a gauge pressure of 250KPa and 135°C for 3 hours;

[0056] 2) Add the product obtained in step (1) into n-hexane according to the ratio of the mass ratio of mushroom fruiting body powder to n-hexane of 1:15, and use a microwave oven with a microwave power of 800W and an extraction time of 40s to filter to obtain fatty acid extraction liquid and the residue after extraction;

[0057] 3) Concentrating the fatty acid extract in a rotary evaporator under the conditions of 30°C and a gauge pressure of -0.06Mpa to obtain a fatty acid extract of Lentinus edodes;

[0058] The extracted residue was added to an aqueous solution of 3.0% papain at a mass concentration of 15 times the mass, and was ultrasonically tr...

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Abstract

The invention discloses a method for comprehensive extraction of effective constituents in mushrooms. The method comprises the following steps: 1) crushing mushrooms and carrying out autoclaving; 2) adding the mushrooms in n-hexane or petroleum ether and carrying out extraction and filtration so as to obtain an aliphatic acid extract liquid and an extraction residue; 3) concentrating the aliphatic acid extract liquid so as to obtain an aliphatic acid extract of the mushrooms, adding the extraction residue into an aqueous solution of papain and successively carrying out ultrasonic treatment and a reaction so as to obtain a decomposition liquid; 4) concentrating the decomposition liquid so as to obtain an extract of lentinan and amino acid; 5) dissolving the extract of lentinan and amino acid with distilled water; 6) carrying out ultrafiltration so as to obtain a solution with interception of 8000 to 14000 and a filtered solution and carrying out concentration so as to obtain an extract of amino acid; and 7) concentrating an intercepted solution, adding ethanol for deposition, carrying out centrifugal separation, discarding a supernatant and collecting a precipitate so as to obtain a polysaccharide extract of the mushrooms. Compared with traditional methods, extraction amounts of aliphatic acid, polysaccharide and amino acid in the mushrooms are substantially increased by using the method provided by the invention.

Description

technical field [0001] The invention relates to a method for comprehensively obtaining active ingredients from shiitake mushrooms. Background technique [0002] Lentinus edodes is a fungus belonging to the genus Lentinus genus of the Agaricaceae Basidiomycetes. my country is the largest producer of shiitake mushrooms in the world, and its output accounts for 70% of the world's total output. Modern medicine believes that shiitake mushrooms are "a medicament for compensating vitamin D, preventing rickets, and treating anemia." There are also records of shiitake mushrooms in ancient books: "Compendium of Materia Medica" records that shiitake mushrooms are "dry and flat, non-toxic"; , treat wind and break blood"; in "The Classic of Fengyuan", shiitake mushrooms "greatly benefit stomach qi". [0003] Shiitake mushrooms contain a lot of nutrients and have certain medicinal value. Scientific research shows that shiitake mushrooms have biological activities of anti-cancer, anti-o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/04C11B1/10C08B37/00C07C229/00C07C227/40C07C227/28
Inventor 陈海霞张宁章钰马利帅
Owner 万洲胶粘制品(江苏)有限公司