Rhynchostylis protocorm rapid breeding method

Abstract

The invention relates to a rhynchostylis protocorm rapid breeding method, and belongs to the field of renascent herb breeding. The method comprises the following steps: taking rhynchostylis seeds as the explants, then carrying out a complete breeding process: from seeds to protocorm (protocorm-like bodies) to multiple shoots to whole plants; and results show that: under conditions of a temperature of 23 DEG C to 27 DEG C, a light intensity of 1500 to 2000 lx, and an illumination time of 12 h / d, the optimum culture medium is MS+6-BA(1.0 mg / L)+NAA(0.1 mg / L), the optimum proliferation culture medium is MS+6-BA(3.0 mg / L)+NAA(0.1 mg / L), and the rooting culture medium is 1 / 2MS+NAA(1.0 mg / L). The method can obtain a large amount of high-quality seedlings in a short time; utilizes rhynchostylis immature seeds as the explants, uses biological technologies such as tissue culture, and the like, to induce, proliferate, and promote root growth on rhynchostylis protocorm-like bodies, then carries out processes of seedling strengthening, root culturing, and transplanting on tissue cultured seedlings, and provides a novel way for industrial rhynchostylis breeding.

Description

technical field [0001] The invention relates to a method for rapid propagation of protocorms of orchid orchids, which belongs to the field of perennial herbaceous plants of the genus Orchidaceae. Background technique [0002] Orchidaceae Foxtail Orchid is a perennial herb, a typical tropical epiphytic orchid, and one of the excellent germplasm resources of wild orchids in Hainan. It has strong adaptability, easy cultivation, and the flowering period is around the traditional Spring Festival in my country. It is an excellent flower for celebrating the New Year and is popular with the masses. However, due to the excessive logging of tropical forests in its growth environment, its suitable environment has seriously deteriorated, and people have over-collected and sold it in pursuit of immediate benefits, resulting in a sharp decline in the number of wild foxtail orchids, which has seriously affected the composition of the foxtail orchid ecological group. Nearly endangered. Bec...

AI Technical Summary

Problems solved by technology

However, due to the excessive logging of tropical forests in its growing environment, its suitable environment has seriously deteriorated, and people have over-collected and sold it in pursuit of immediate benefits, resulting in a sharp decline in the number of wild fox-tail orchids, which seriously affected the composition of the fox-tail orchid ecological group. near endangered

Because its seeds are not easy to germinate under natural conditions, the division method is often used for conventional propagation, but this method has a low propagation coefficient and slow propagation speed, which is far from meeting market demand.