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Frozen semen diluent of equus animals and preparation method thereof

A technology of freezing semen and preparation methods, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of low conception rate and short storage time, and achieve the goal of improving quality, improving freeze resistance, and avoiding damage Effect

Active Publication Date: 2014-03-26
ILI RUIDE JUNFA BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on semen dilutions of equine animals is also becoming more and more effective, but there are still many disadvantages, mainly in the short storage time of semen dilutions for sperm motility, for example, the storage time at room temperature is less than 24 hours (the activity rate is lower than 0.3), and the storage time at low temperature is less than 24 hours. Less than 96 hours (viability rate lower than 0.3); low conception rate, etc.

Method used

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  • Frozen semen diluent of equus animals and preparation method thereof

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Effect test

Embodiment 1

[0029] A diluent of frozen semen of the genus equine, the diluent includes a base fluid, and every 100ml of the base fluid includes the following raw materials: D-lactose monohydrate 0.3g, prostaglandin 40μg, EDTA 0.15g, bee pollen aqueous solution 30ml, transparent 0.8 g of hyaluronic acid, 0.5 ml of squalane, and the rest in double distilled water;

[0030] The diluent also includes egg yolk liquid with a volume ratio of 7.5%, glycerol with a volume ratio of 2.5%, penicillin at 1000 units / ml and streptomycin at 1000 μg / ml.

[0031] Its preparation method comprises the following steps:

[0032] (1) Soak 20g of bee pollen in liquid nitrogen for 5 minutes, filter it out, add 60ml of double distilled water, 20kHz ultrasonic treatment for 20 minutes, centrifuge at 800g / min for 10 minutes to take the supernatant, and pass the obtained supernatant through a 5000D molecular sieve to obtain bee pollen aqueous solution;

[0033] (2) Dissolve 0.3g of D-lactose monohydrate, 40μg of pr...

Embodiment 2

[0037]A diluent of frozen semen of an equine animal, the diluent includes a base fluid, and every 100ml of the base fluid includes the following raw materials: D-lactose monohydrate 0.5g, prostaglandin 70μg, EDTA 0.25g, bee pollen aqueous solution 60ml, transparent Alcohol 1.1 g, squalane 0.7ml, the rest is double distilled water;

[0038] The diluent also includes egg yolk liquid with a volume ratio of 5%, glycerol with a volume ratio of 3%, penicillin at 1000 units / ml and streptomycin at 1000 μg / ml.

[0039] Its preparation method comprises the following steps:

[0040] (1) Soak 30g of bee pollen in liquid nitrogen for 8 minutes, filter it out, add 120ml of double distilled water, 25kHz ultrasonic treatment for 25 minutes, centrifuge at 1000g / min for 15 minutes to take the supernatant, and pass the obtained supernatant through a 5000D molecular sieve to obtain bee pollen aqueous solution;

[0041] (2) Add 0.5g of D-lactose monohydrate, 70μg of prostaglandin, 0.25g of EDTA,...

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Abstract

The invention discloses a frozen semen diluent of equus animals and a preparation method thereof. The diluent at least comprises a basic solution prepared from the following raw materials: D-lactose monohydrate, prostaglandin, EDTA (Ethylene Diamine Tetraacetic Acid), a bee pollen aqueous solution, hyaluronic acid and squalane. The diluent also comprises egg yolk liquid, glycerin, penicillin and streptomycin. The preparation method comprises the following steps: (1) preparing the bee pollen aqueous solution; (2) preparing the basic solution; (3) adding the egg yolk liquid, the glycerin, the penicillin and / or the streptomycin. By adopting the technical scheme, the preparation method disclosed by the invention has the benefits that the bee pollen aqueous solution, the hyaluronic acid, the squalane and the like are creatively combined for preparation of the frozen semen diluent of the equus animals to ensure that the forming of large ice crystals in semen freezing process is prevented to the maximum limit, so that the damage of ice crystallization to semen is avoided, the survival rate and the acrosome intact rate of frozen semen are remarkably increased, the quality of the frozen semen is further improved and the freezing tolerance of spermatid is increased.

Description

[0001] technical field [0002] The invention relates to the technical field of animal frozen semen production, in particular to an equine animal frozen semen diluent and a preparation method thereof. [0003] Background technique [0004] At present, the breeding of Equus animals in my country is mainly carried out in two ways: natural mating and artificial assisted mating. However, in the case of natural mating or artificial assisted mating, generally a stallion can only be mated with 20-30 mares. Stallion utilization is severely limited. Therefore, the development and application of semen dilution, preservation and artificial insemination technology is very important. [0005] In recent years, the research on livestock semen dilutions has achieved gratifying results, especially in ruminants such as cattle and sheep. The research on semen dilutions of equine animals is also becoming more and more effective, but there are still many disadvantages, mainly in the short stor...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 邹敬清邹志钢孙吉胜李中航张志鹏荣美洁
Owner ILI RUIDE JUNFA BIOTECH
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