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Kit for detecting susceptibility gene of glaucoma

A susceptibility gene, kit technology, applied in the fields of molecular biology and medicine

Inactive Publication Date: 2014-03-26
浙江爱易生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Statistical analysis shows that people with the variant gene have a significantly higher risk of developing exfoliation glaucoma than normal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Use of detection kits

[0031] Step 1: Extraction of DNA template

[0032] Vacuum blood collection tubes are used to collect human peripheral blood, and the genomic DNA in the blood is extracted.

[0033] Step 2: Real-time quantitative PCR reaction

[0034] Use a fluorescent quantitative PCR detection kit that can detect glaucoma susceptibility genes, which includes the following primer pairs and fluorescent probe pairs:

[0035] Sense primer 1: 5'- TCCGTCTCCCCAGCAA - 3' (SEQ ID NO: 1)

[0036] Antisense primer 1: 5'-CGAAGCCGAGACCGA– 3' (SEQ ID NO: 2)

[0037] Sense Primer 2: 5'-TGACAGTCCTTTTTCT - 3' (SEQ ID NO: 3)

[0038] Antisense Primer 2: 5'- AATTATATTAACAAA - 3' (SEQ ID NO: 4)

[0039] Probe 1 with VIC fluorophore: 5'-CACGGGGaCTCCGC-3' (SEQ ID NO: 5)

[0040] Probe 1 with FAM fluorophore: 5'-CACGGGGgCTCCGC-3' (SEQ ID NO: 6)

[0041] Probe 2 with VIC fluorophore: 5'-GTATTGTaTTTGTT-3' (SEQ ID NO: 7)

[0042] Probe 2 with FAM fluorophore: 5'-GTATTG...

Embodiment 2

[0050] Example 2. Services for glaucoma susceptibility gene testing for patients

[0051] Step 1: DNA Extraction

[0052] The laboratory physicians of the hospital collect peripheral blood from the subjects, use vacuum blood collection tubes to collect peripheral blood, and extract genomic DNA from it.

[0053] Step 2: Genotyping Assays

[0054] Using the kit provided by the present invention, the SNP site No. rs3825942 on the LOXL1 gene of the subject's genomic DNA and the SNP site No. rs4236601 on the CAV1 / CAV2 gene are respectively subjected to fluorescent quantitative PCR detection to determine the two SNPs sites genotype.

[0055] Step 3: Risk analysis for glaucoma

[0056] Through the analysis of the SNPs genotypes of the tested subjects, an analysis report of the risk of suffering from glaucoma is issued. The report details the risk of the subject suffering from glaucoma, and the doctor explains and interprets the analysis report sheet of the risk of suffering from gl...

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Abstract

The invention discloses a kit for detecting a susceptibility gene of glaucoma. The kit comprises specific primer pairs, specific fluorescent probe pairs, conventional components and the like, wherein the specific primer pairs and the specific fluorescent probe pairs are used for simultaneously detecting a No.rs3825942 SNP (single nucleotide polymorphism) site on an LOXL (lysyl oxidase-like)1 gene and a No.rs4236601 SNP site on a CAV (chicken anemia virus)1 / CAV2 gene; the conventional components are used for fluorescent quantitative PCR (polymerase chain reaction) detection. The kit is used for evaluating the risk of suffering from glaucoma by individuals by simultaneously detecting SNP site genotypes of LOXL1 and CAV1 / CAV2 closely related to glaucoma.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, and more specifically, the present invention relates to a kit for detecting susceptibility genes of glaucoma, by simultaneously detecting single nucleotide polymorphism positions of genes LOXL1 and CAV1 / CAV2 associated with glaucoma Point genotypes were used to assess an individual's risk of developing glaucoma. Background technique [0002] Glaucoma is the third leading cause of blindness in the world, and its typical clinical manifestations are optic atrophy, progressive visual field defect and increased intraocular pressure. Although the exact pathogenesis is still unknown, according to the familial incidence of primary glaucoma, the high incidence among relatives of glaucoma, and studies of twins, all indicate that genetic factors play an important role in the pathogenesis of glaucoma. With the deepening of the research and application of molecular genetics and molecula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 任峻张华忠
Owner 浙江爱易生物医学科技有限公司
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