Operation instrument for optical tweezers

Abstract

The invention relates to an operation instrument for optical tweezers. The operation instrument comprises a laser source, a convex lens, a plane mirror, a two-tone spectroscope, optical wedges, a focusing objective lens, a sample table, a sample pool, an illuminating light source and a CCD camera. Light beams are emitted by the laser source, expanded and shaped by a light beam coupler, and then are converged and irradiated by the plane first mirror, the two-tone spectroscope, the two deflection optical wedges and the focusing objective lens into the sample pool to capture target particles, the first optical wedge and the second optical wedge are driven by a motor to deflect around the Y axis and the X axis respectively in an orthogonal mode, the deflection angle can be measured through an angle sensor, the deflection of the light beams is controlled, and the target particles are moved; the particle capturing and moving process can be visually acquired through the CCD camera and a displayer. When the particles move in the plane parallel to the XOY plane, the sample pool does not need to move, namely, the particles are not affected by external vibration in the moving process; in addition, due to the fact that the two orthogonal deflection optical wedges can achieve the light beam deflection hundredfold-order reduction ratio, the deflection directions of the light beams can be accurately controlled, and the light beams can capture the particles accurately and carry the particles to move firmly.

Description

technical field [0001] The invention relates to a technique for operating particle movement by optical tweezers, in particular to a technique for precisely controlling particle movement by using a deflection double optical wedge, and in particular to an optical tweezer operating instrument. Background technique [0002] Optical tweezers is a physical tool based on the mechanical effect of laser, which uses the optical potential well formed when the strongly converged light field interacts with particles to capture particles. [0003] The capture and holding of particles by optical tweezers has the characteristics of no mechanical contact and no damage, which also makes optical tweezers technology popular in the fields of biomedicine and biotransgenic technology such as cell separation and cell fusion. However, the precise tracking and control of particle movement is the key technology for the operation of optical tweezers. [0004] The operation of optical tweezers in the p...

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Problems solved by technology

However, the deflection angle of the reflected beam of this device is multiplied by the angle of t

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