Micro-scale substance separating method and capillary column transverse eletrochromatography separating device

Abstract

The invention discloses a micro-scale substance separating method and a capillary column transverse eletrochromatography separating device. The separating method comprises the step of introducing a sample into a separating column of a separating device, wherein the separating column is composed of a capillary tube which is used as an external electrode and a conductive material which is used as an internal electrode and penetrates through the capillary tube along the axial direction; the internal electrode and the external electrode are coaxial, and are respectively connected with the two ends of a power supply; the separating column transversely applies electric fields upwards, and electrophoresis effect is utilized for transversely transferring to-be-separated substances, so that contact probability of the to-be-separated substances and a column surface is increased, and separation is realized according to difference of interaction force sizes of the to-be-separated substances and the column surface; an absorption analyzing process of the to-be-separated substances and the column surface is completed by switching direction of the electric fields, so that resolution among the to-be-separated substances is improved. According to the invention, an electrophoretic technique and a chromatographic technique are integrated to realize effective separation of different substances, operation of the separating method and manufacturing of the special-purpose device are simple; and moreover, online detection can be realized.

Description

technical field [0001] The invention relates to a method for separating microscale substances and special equipment thereof, in particular to a separation method for microscale substances and a capillary column transverse electrochromatographic separation device which integrates electrophoresis technology and chromatographic technology. Background technique [0002] At present, the separation and screening of micro-scale substances such as cells, biomacromolecules, synthetic particles, colloids, etc. has become a preferred main research content in various contemporary research fields. Traditional separation techniques are difficult to separate samples with a wide range of sizes at the same time, and it is difficult to obtain target substances of a single size, which will bring inconvenience to follow-up research. In order to achieve the separation of micro-scale substances, some non-blocking and non-filling separation systems have been established, and most of them are conti...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiency of low separation degree of micro-scale substances in non-packed separation systems, and provide a separation method of micro-scale substances and its special equipment capillary column transverse electrochromatographic separation device. The separation column introduces an electric field laterally, and the column surface introduces chroma

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