Micro-scale substance separating method and capillary column transverse eletrochromatography separating device

Abstract

The invention discloses a micro-scale substance separating method and a capillary column transverse eletrochromatography separating device. The separating method comprises the step of introducing a sample into a separating column of a separating device, wherein the separating column is composed of a capillary tube which is used as an external electrode and a conductive material which is used as an internal electrode and penetrates through the capillary tube along the axial direction; the internal electrode and the external electrode are coaxial, and are respectively connected with the two ends of a power supply; the separating column transversely applies electric fields upwards, and electrophoresis effect is utilized for transversely transferring to-be-separated substances, so that contact probability of the to-be-separated substances and a column surface is increased, and separation is realized according to difference of interaction force sizes of the to-be-separated substances and the column surface; an absorption analyzing process of the to-be-separated substances and the column surface is completed by switching direction of the electric fields, so that resolution among the to-be-separated substances is improved. According to the invention, an electrophoretic technique and a chromatographic technique are integrated to realize effective separation of different substances, operation of the separating method and manufacturing of the special-purpose device are simple; and moreover, online detection can be realized.

Description

technical field [0001] The invention relates to a method for separating microscale substances and special equipment thereof, in particular to a separation method for microscale substances and a capillary column transverse electrochromatographic separation device which integrates electrophoresis technology and chromatographic technology. Background technique [0002] At present, the separation and screening of micro-scale substances such as cells, biomacromolecules, synthetic particles, colloids, etc. has become a preferred main research content in various contemporary research fields. Traditional separation techniques are difficult to separate samples with a wide range of sizes at the same time, and it is difficult to obtain target substances of a single size, which will bring inconvenience to follow-up research. In order to achieve the separation of micro-scale substances, some non-blocking and non-filling separation systems have been established, and most of them are conti...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiency of low separation degree of micro-scale substances in non-packed separation systems, and provide a separation method of micro-scale substances and its special equipment capillary column transverse electrochromatographic separation device. The separation column introduces an electric field laterally, and the column surface introduces chromatographic action. After microscale substances enter the separation column, they can undergo lateral electromigration under the action of the electric field. Through the electromigration behavior of the substance, the contact probability between the particles and the column surface is increased, thereby improving the chromatographic effect. contribution, and finally achieve the purpose of improving the separation

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