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Cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof

A technology of polyadenosine and binding protein, which is applied in the field of vaccine preparation, can solve problems such as unsatisfactory prognosis, and achieve the effects of simple preparation method and good application prospect.

Inactive Publication Date: 2014-04-09
中国人民解放军南京军区福州总医院四七六医院
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Problems solved by technology

[0002] Glioma is the most common tumor of the central nervous system in the human body. Although various methods have been adopted for active treatment, the prognosis is still not ideal

Method used

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  • Cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof
  • Cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof
  • Cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof

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Embodiment 1

[0030] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. For the experimental methods that do not specify specific conditions in the preferred embodiments, usually according to conventional conditions, see Molecular Cloning Experiment Guide (Third Edition, written by J. Sambrook et al., translated by Huang Peitang et al., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0031] 1. Cloning of CPEB4 full-length cDNA

[0032] According to the CPEB4 gene sequence with GenBank accession number NM_030627, the following primers were designed and synthesized to amplify the full-length CPEB4 cDNA with restriction sites at both ends: F1: 5’-aat ctgcag atgggggattacgg -3' (SEQ ID No.1), the underlined part is the PstⅠ restriction site; R1: 5'-tac gaattc gctggaactaa-3' (SEQ ID No.2), the underlined part is the EcoRI restriction site.

[0033] Glioma cells from postoperative patie...

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Abstract

The invention discloses a cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof. The vaccine is a ISCOM-DNA compound, the preparation method of the vaccine comprises: cloning of full-length DNA of cytoplasmic polyadenylation element-binding protein 4, cloning of full-length DNA of tumor necrosis factor BAFF, construction of a recombinant vector pStar-CPEB4 / BAFF, construction of a pStar-CPEB4 / BAFF engineering bacteria, inducible expression of CPEB4, and preparation of the ISCOM-DAN compound. The vaccine has good application prospect on neuroglioma controlling. The vaccine has the advantages of being efficient, low in toxicity, simple in preparation method and the like, and is applicable to medicines for tumour immunotherapy.

Description

technical field [0001] The invention relates to an immunoenhanced vaccine of cytoplasmic polyadenylation component binding protein 4, and also relates to a preparation method and application of the vaccine. Background technique [0002] Glioma is the most common tumor of the central nervous system in the human body. Although various methods have been adopted for active treatment, the prognosis is still not ideal. Cytoplasmic polyadenylation element-binding proteins (CPEBs) are a group of RNA-binding proteins that mediate cytoplasmic polyadenylation and translation of mRNA. In recent years, it has been found that it is abnormally expressed in most tumor cells. With the development of research on the relationship between CPEBs and tumors, foreign studies have reported that CPEB4 is positively expressed in glioblastoma, and its positive rate is positively correlated with the degree of tumor malignancy. Therefore, CPEB4 is expected to be a new therapeutic target for glioma. ...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K48/00A61K9/127A61P35/00C12N15/81C12R1/84
Inventor 袁邦清陈羽建沈汗超林立吴贤群
Owner 中国人民解放军南京军区福州总医院四七六医院
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