Chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment and expression and application thereof

A staphylococcus, surface protein technology, applied in the field of genetic engineering technology and diagnostic reagents, can solve the problems of identification results, such as only qualitative but not quantitative, slow speed and complex processing.

Active Publication Date: 2014-04-16
李越希
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the samples should be enriched and cultured according to the type of pathogenic bacteria to be detected. The identification results are generally only qualitative but not quantitative, and the time required is long and slow. Generally, it takes 3-4 days to infer the results, and accurate identificati

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment and expression and application thereof
  • Chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment and expression and application thereof
  • Chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment and expression and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0049] Detailed description of the embodiments of the present invention:

[0050] Analysis, gene synthesis and expression of the epitope of the surface protein FnBPA of Staphylococcus aureus

[0051] Through computer analysis of the full-length amino acid sequence of the surface protein FnBPA of Staphylococcus aureus, the strong antigen epitope in the surface protein FnBPA of Staphylococcus aureus was screened out, and the codons preferred by prokaryotes were selected and translated into the corresponding nucleotide sequence. Chemistry Synthesize a new gene sequence for the strong epitope of the surface protein FnBPA of Staphylococcus aureus. The gene fragment was cloned into the plasmid pGEX4T-2 BamH I and Xho Between the I sites, it is consistent with the translation framework of the start codon on the vector to express a fusion protein. The recombinant plasmid was transformed into Escherichia coli BL21 (DE3), and an engineered strain was screened to efficiently express the s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment, as well as expression and application thereof, belonging to the fields of genetic engineering technologies, antibodies and kits. According to the chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment provided by the invention, a strong antigen epitope, namely 133 amino acids in total from 745th amino acid to 877th amino acid in the staphylococcus aureus surface protein FnBPA are screened out through computer analysis, a codon preferred by prokaryotes is selected, a brand new gene sequence of the antigen epitope is chemically synthesized, the gene fragment is expressed by utilizing the genetic engineering technology, and the strong antigen epitope fragment of the staphylococcus aureus surface protein FnBPA is prepared. The expressed strong antigen epitope fragment of the staphylococcus aureus surface protein FnBPA can be used for detection of staphylococcus aureus antibodies and immune preparation of anti-staphylococcus aureus monoclonal antibodies, polyclonal antibodies and the like.

Description

technical field [0001] The invention relates to the chemically synthesized surface protein FnBPA gene fragment of staphylococcus aureus and its expression and application, and uses genetic engineering technology to prepare the surface protein FnBPA of recombinant staphylococcus aureus. Through computer analysis, FnBPA fragments containing strong antigenic epitopes are screened out, codons preferred by prokaryotes are selected, a new gene sequence is chemically synthesized, and expressed by genetic engineering technology. The expressed protein can be used for vaccines and rapid detection methods for Staphylococcus aureus The establishment of etc., the present invention relates to the field of genetic engineering technology and diagnostic reagents. Background technique [0002] Staphylococcus aureus is an opportunistic pathogen that can cause disease in humans and animals. It parasitizes human skin and mucosal surfaces as a normal flora and accounts for the majority of nosoco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/31C12N15/70C07K14/31C07K16/12G01N33/569
Inventor 李越希李丙军许桂丽马颖张素芬袁敬宇徐悦玥潘英李素芹陈乐如蔡冉周洁尚彦红
Owner 李越希
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap