Microfluidic system for reliably controlling the concentration profile of molecules to stimulate targets
A microfluidic system and concentration distribution technology, applied in the field of microfluidics, can solve problems such as difficult observation of living cells
Inactive Publication Date: 2016-02-24
CENT NAT DE LA RECHERCHE SCI +4
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- Description
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Problems solved by technology
Therefore, it is difficult to observe the response of living cells placed on the membrane with suitable optical devices
Method used
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[0064] The present invention relates to a microfluidic system for controlling the concentration profile of molecules to be able to stimulate a target, for example formed by a population of living cells, said system comprising a microfluidic device and for supplying the device with molecules capable of stimulating the target at least one body of at least one fluid.
[0065] see figure 2 A microfluidic device is described, and the fabrication process of the device is described with reference to Figures 3(a) to 3(d) and 4(a) to 4(c). then see Figure 5(a) and 6(a) Specific forms of microfluidic channels that can be used within the device are described as non-limiting examples.
[0066] figure 2 The microfluidic device 1 according to the invention is shown in partial section, perspective view.
[0067] The microfluidic device 1 comprises a cover 2, advantageously rigid, provided with two orifices 21, 22 and a side wall 3, advantageously made of photocurable and / or thermally c...
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Abstract
The invention relates to a microfluidic system for controlling the concentration profile of molecules capable of stimulating targets formed, for example, from assemblies of living cells, the system comprising: a microfluidic device (1) comprising a device for at least one fluid at least one microfluidic channel (4) of at least one inlet hole (21) and at least one outlet hole (22); at least A device; at least one chamber (8) or another microfluidic channel comprising a base (6) for receiving said target; and connecting said chamber (8) or another microfluidic channel to said at least one microporous film (5) separated by the microfluidic channel (4), the microporous film (5) being arranged away from the base (6), so that when the supply mechanism supplies the microfluidic channel (4) When said at least one fluid is supplied to flow in laminar flow in contact with said microporous membrane (5), the molecules capable of stimulating the target after having passed through the microporous membrane (5) through the chamber (8) or another After the microfluidic channel, diffusion is then followed to finally form a stable concentration profile in the chamber (8) or in the further microfluidic channel.
Description
technical field [0001] The present invention relates to the field of microfluidics. Background technique [0002] Microfluidic application systems have micron dimensions, with dimensions typically ranging from tens of microns to hundreds of microns. [0003] The system can be used in many fields, for example, cell diagnostic tests, medical product development, basic biological sciences or cosmetology. [0004] In said field, there is an increasing demand for microfluidic systems for the quantitative determination of the response (response) of living cells to certain molecules, and in particular to spatially and temporally controlled concentration profiles. [0005] For example, there may be the problem of measuring the response of cancer cells to molecules used in chemotherapy. Precise measurement of this reaction requires the exercise of control over the molecules that produce the reaction. The control may relate to the number of molecules interacting with the cancer cel...
Claims
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IPC IPC(8): B01L3/00C12M3/06G01N33/50
CPCB01L3/502753B01L2300/0681C12M23/16B01J2219/00639B01L2300/0848B01L2300/0627B01L3/502715B01L2200/0647B01L2300/0851B01L3/502761B01L2200/0652B01L3/5027B01L2300/087B01L2200/027B01L2300/0816B01L2300/163G01N33/5008
Inventor M·达昂M·莫雷尔J-C·加拉V·施图德D·巴尔托洛
Owner CENT NAT DE LA RECHERCHE SCI