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Lactobacillus plantarum capable of alleviating copper toxicity and purpose thereof

A technology of Lactobacillus plantarum and drugs, applied in the direction of medical preparations containing active ingredients, applications, bacteria, etc., can solve problems affecting internal organs and nervous system, copper poisoning, etc., and achieve the effect of alleviating copper toxicity

Active Publication Date: 2014-04-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Copper is one of the essential trace elements in the human body. It is widely distributed in biological tissues, most of which exist in organic complexes, many of which are metalloproteins, and play a functional role in the form of enzymes; however, excessive copper will accumulate in the human body and cause copper poisoning , affecting the internal organs and nervous system

Method used

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  • Lactobacillus plantarum capable of alleviating copper toxicity and purpose thereof
  • Lactobacillus plantarum capable of alleviating copper toxicity and purpose thereof
  • Lactobacillus plantarum capable of alleviating copper toxicity and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1 : Tolerance test of Lactobacillus plantarum CGMCC NO.8246 to copper ions

[0105] Under sterile conditions, add 0.1965 g CuSO to 1 L of water 4 ·5H 2 O, obtain the copper ion aqueous solution of 50mg / L. Use this aqueous solution of copper ions to dissolve the solid ingredients of the MRS medium to obtain an MRS liquid medium containing 50 mg / L copper ions. MRS medium is a medium well known to those skilled in the art, and it contains peptone fish powder, yeast extract, glucose, sodium acetate, diammonium citrate, Tween 80, magnesium sulfate, manganese sulfate, pH6.2~6.4.

[0106] MRS liquid medium containing 50 mg / L, 150 mg / L and 500 mg / L copper ion concentrations were prepared in the same manner as described above. Inoculate the Lactobacillus plantarum CGMCC NO.8246 that has entered the stationary phase into the copper-containing MRS liquid medium at a 2% inoculum based on the weight of the copper-containing MRS liquid medium, and cultivate it at 37°C wi...

Embodiment 2

[0108] Example 2 : Experiment of adsorption capacity of Lactobacillus plantarum CGMCC NO.8246 to copper ions

[0109] According to the screening standard of acid resistance (able to grow in pH3.0 environment), 16 strains of lactic acid bacteria were screened from traditional food in my country, such as kimchi and fermented milk wine, and these 16 strains of lactic acid bacteria were purified and activated under sterile conditions. And when lactic acid bacteria are activated, MRS medium is used. The obtained thalline was transferred to the container containing the 50mg / L copper ion concentration solution, while the blank group was transferred to deionized water. Determine the volume of the liquid in the blue cap bottle according to the mass of the thallus, so that the final concentration of the thallus reaches 1 g / L. The above samples were incubated at a temperature of 37° C. for 2 h. Use a Beckman centrifuge to centrifuge at a speed of 6000r / min for 20min, then wash with st...

Embodiment 3

[0111] Example 3 : Tolerance dose experiment of Lactobacillus plantarum CGMCC NO.8246 fed to mice

[0112] Resuspend the freeze-dried powder of Lactobacillus plantarum CGMCC NO.8246 in skim milk powder to make a concentration of 2.0×10 9 CFU / mL suspension. Take 10 healthy male C57 mice of about 20 g, give the suspension of this concentration orally once a day, observe for a week, and record the death and body weight.

[0113] The results of these tests are listed in Table 1. These results indicate that feeding concentrations of 2.0×10 9 CFU / mL of Lactobacillus plantarum CGMCC NO.8246 had no significant effect on the mice, the body weight increased significantly, and no death occurred. The appearance of the mice had no obvious pathological symptoms.

[0114] Table 1: Feeding concentration 2.0 x 10 9 Changes of CFU / mL Lactobacillus plantarum CGMCC NO.8246 on the body weight of mice

[0115]

[0116] Note: -: no death of mice

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Abstract

The invention discloses a Lactobacillus plantarum, which is preserved on September 23, 2013 in China General Microbiological Culture Collection Center belonging to China?Committee?for?Culture?Collection?of?Microorganisms, and has a preservation number of CGMCC NO.8246; and the preservation address is Institute of microbiology of Chinese Academy of Sciences, No.3, Yard No.1, West Beichen Road, Chaoyang District, Beijing. The Lactobacillus plantarum provided by the invention has acid resistance, good in vitro tolerance to copper ions, can tolerate copper ion solution with initial concentration of 50 mg / L, has strong adsorption of copper ions, and can reduce the copper content in liver, kidney and brain of copper-exposed mice, mitigate the pathological symptoms of copper-exposed mice, alleviate liver injury and lower AST and ALT contents in serum of mice. The Lactobacillus plantarum is used for the preparation of pharmaceutical compositions and fermented food to alleviate the toxicity of copper, and has wide application prospect.

Description

technical field [0001] The invention relates to a plant lactobacillus and its use, in particular to a plant lactobacillus capable of alleviating copper toxicity and its use. Background technique [0002] Copper is one of the essential trace elements in the human body. It is widely distributed in biological tissues, most of which exist in organic complexes, many of which are metalloproteins, and play a functional role in the form of enzymes; however, excessive copper will accumulate in the human body and cause copper poisoning , affecting the internal organs and nervous system. With the development of industry, the mining and smelting of copper-zinc mines and metal processing have become more frequent, and the content of copper in water and soil has increased year by year, causing pollution of plants (such as poor growth of rice and barley, and pollution of grain grains) and animals. Accumulate in the body (such as liver, kidney, brain, etc.), and finally accumulate in the h...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P39/02A23C9/123A23C13/16A23C19/032A23C11/10A23L1/20A23L1/24A23L1/212C12R1/25A23L11/50A23L11/60A23L19/00A23L27/60A61K35/747
Inventor 陈卫翟齐啸田丰伟王刚张秋香范大明刘小鸣赵建新张灏李肖肖
Owner JIANGNAN UNIV
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