A method for extracting epigallocatechin gallate from tea leftovers
A technology of epigallocatechin and gallate, which is applied in the field of extraction of epigallocatechin gallate, can solve the problems of difficult separation, difficulty in production of epigallocatechin gallate, and large production difficulty, and achieves Effects of reduced energy consumption, enhanced compatibility and continuity, and reduced processing costs
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Embodiment 1
[0034] Embodiment 1: A kind of method of the present invention extracts epigallocatechin gallate from leftovers of tea leaves, comprises the following major steps:
[0035] a. Extraction: Put the leftovers of tea leaves into an extraction tank after being crushed, add ethanol, stir after soaking and heat for extraction three times, combine the first two extracts, and the third extract is used for the next batch of extraction;
[0036] b. Concentration: the first two extracts are concentrated under reduced pressure to ethanol-free;
[0037] c. Upper macroporous adsorption resin: pass the decompression concentrate through the macroporous adsorption resin column, wash once with ion-free water, then elute with ethanol, and collect the eluate; the eluate is concentrated under reduced pressure to no ethanol;
[0038] d. Upper medium-pressure chromatography column: pump the decompressed concentrated solution into the medium-pressure chromatography column with a constant flow pump, wa...
Embodiment 2
[0041] Embodiment 2: A kind of method of the present invention extracts epigallocatechin gallate from leftovers of tea leaves, comprises the following main steps:
[0042] 1. Extraction: Grind 1KG tea leaves into coarse powder, put them into an extraction tank, add 20L of 60% ethanol each time, soak for 1 hour, stir and heat to 80°C, keep warm for 30min, and filter with a 300-mesh filter cloth to obtain The extract and filter residue were extracted twice according to the above conditions. The first two extracts were combined to about 35L, and the third extract of about 19L was used for the next batch of extraction.
[0043] 2. Concentration: Concentrate the first two extracts under reduced pressure below 50°C until there is no ethanol to obtain about 6L of concentrate.
[0044] 3. Treatment of D101 macroporous adsorption resin: Soak 10LD101 macroporous adsorption resin with 95% ethanol for 24 hours, after fully swelling, wet-pack the column, rinse with 95% ethanol until the el...
Embodiment 3
[0050] Embodiment 3 A method of extracting epigallocatechin gallate from tea leftovers of the present invention comprises the following main steps:
[0051] 1. Extraction: crush 10KG tea leaves into coarse powder, put them into an extraction tank, add 180L of 70% ethanol each time, soak for 2 hours, stir and heat to 50°C, keep warm for 30min, and filter with a 300-mesh filter cloth to obtain The extract and the filter residue were extracted twice according to the above conditions, and the combined first two extracts were about 330L, of which about 172L of the third extract was used for the next batch of extraction.
[0052] 2. Concentration: Concentrate the first two extracts under reduced pressure below 50°C until there is no ethanol to obtain about 65L of concentrate.
[0053] 3. Treatment of HPD100 macroporous adsorption resin: Soak 100LHPD100 macroporous adsorption resin with 95% ethanol for 24 hours, after fully swelling, wet-pack the column, rinse with 95% ethanol until ...
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