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A new process for preparing Poria cocos polysaccharide extract by membrane separation and purification technology

A Poria polysaccharide and membrane separation technology, which is applied in the field of natural product extraction and separation, can solve the problems of long extraction time and low extraction rate, and achieve the effects of shortening extraction time, improving purity, and mild conditions

Active Publication Date: 2016-02-10
WUHAN HUANG MEDICAL SYST BIOLOGICAL MEDICINE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current extraction and separation methods have the advantages of being cheap, non-toxic, and safe to operate, the extraction time is too long and the extraction rate is low, so it is impossible to obtain effective single-component pachyrhin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 5kg of Poria cocos, put it into the extraction tank, add 30kg of deionized water, cover and seal it, control the temperature at 90°C, and the auxiliary ultrasonic power at 10MHz, stir intermittently for 5 minutes per hour, after leaching for 80min, filter out the filtrate, and separate Add 10kg of deionized water for three extractions (boiling for 30 minutes each time), combine the four boiling extracts and filter out a total of 55kg after coarse filtration in a centrifuge, and cool to 30°C; filter the filtrate through a microfiltration membrane PP with a pore size of 5 μm Cotton filtration, operating temperature 25°C, pressure 0.3Mpa, to obtain filtrate 51kg; obtained filtrate was further filtered with polysulfone ultrafiltration membrane PS-10 with a nominal molecular weight cut-off of 10,000 Daltons, operating temperature 25°C, pressure 0.05Mpa, obtained filtrate 49kg; the filtrate was concentrated and freeze-dried to obtain 3.7kg of Poria cocos powder.

Embodiment 2

[0031] Take 10kg of Poria cocos and 100kg of extractant, put them into an extraction tank, control the temperature at 95°C, and use an auxiliary ultrasonic power of 15MHz, stir intermittently for 10 minutes per hour, leaching, after 100min, filter out the filtrate, then add 50kg of extractant to extract for 100min, After the two extracts were combined, they were centrifugally filtered to remove impurities; after cooling 146kg of the filtrate to 30°C, filter it with a microfiltration membrane PP cotton with a pore size of 10μm, operating at a temperature of 30°C and a pressure of 0.5Mpa to obtain 143kg of the filtrate; 30,000 Dalton roll-type polypropylene ultrafiltration membrane HPS-30 was further filtered, the operating temperature was 30°C, and the pressure was 0.2Mpa to obtain 138kg of filtrate; after the filtrate was concentrated, it was freeze-dried to obtain 7.8kg of saffron powder.

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Abstract

The invention relates to a new process for preparing Poria cocos polysaccharide extract by membrane separation and purification technology. The process comprises the following steps: (1) Grinding and sieving Poria cocos into 20-mesh coarse powder, then adding 5 to 10 times deionized water, Obtain the feed liquid; (2) filter the feed liquid obtained in step (1) through a centrifuge; (3) filter the filtrate obtained in step (2) with microfiltration membrane PP cotton; (4) filter the filtrate obtained in step (3) The filtrate is further filtered with a polysulfone hollow fiber ultrafiltration membrane (UF) with a nominal molecular weight cut-off of 10,000 Daltons; (5) the filtrate obtained in step (4) is dried by vacuum freeze-drying; (6) the step ( 5) The prepared polysaccharide is used as an animal model of Drosophila to carry out anti-oxidation and anti-aging research; (7) The polysaccharide prepared in step (5) is used. The invention has simple operation and greatly improves the purity and efficiency of the polysaccharide.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction and separation. The invention relates to a method for extracting, separating and purifying polysaccharides prepared from poria cocos as a raw material, and the efficacy evaluation of hypoglycemic and antioxidative activities. Background technique [0002] Poria cocos belongs to the genus Poria cocos of the family Polyporaceae of the order Aphylloides, and is a fungal traditional Chinese medicine. Sweet, light, flat, heart-guiding, spleen, kidney meridian, has the effects of diuretic and dampness, invigorating the spleen and calming the heart. The main medicinal ingredient in Poria cocos is Poria cocos polysaccharide. Poria cocos polysaccharide has anti-tumor, anti-virus, anti-oxidation, enhancement of immunity, liver protection, hypnosis, anti-inflammation, anti-calculus and other effects, and can be widely used in medical care, food and other fields. Poria cocos polysacchari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 何静仁洪峰刘莉段李歌蔡小双齐敏玉
Owner WUHAN HUANG MEDICAL SYST BIOLOGICAL MEDICINE INC
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