Method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas

A technology of steroid dehydrogenase and Comamonas, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the physiological imbalance of soil nutrient elements, affect the normal physiological metabolism of crops, crop nutrition Balance disorders and other problems, to improve the rhizosphere environment, promote rhizosphere cell division, and exuberant metabolism

Inactive Publication Date: 2014-05-21
山东环亿生物科技有限公司
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

[0005] At present, the phenomenon of soil self-poisoning is relatively serious. The phenomenon of self-poisoning means that some toxins, such as aldehydes, alcohols, hydrocarbons, and accumulated drug residues and steroid hormones, will be produced during the decay and decomposition process of the residues of previous crops. etc., have a significant inhibitory effect on the next stubble crops, affecting the growth of the crops themselves, coupled with the massive reproduct

Method used

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Embodiment Construction

[0047] A method for efficiently producing hydroxysteroid dehydrogenase using Comamonas testosterone of the present invention comprises the following steps:

[0048] Bacteria culture:

[0049] To expand the culture of purchased strains, first configure LB medium, 10 grams of peptone, 5 grams of yeast extract powder, 5 grams of sodium chloride, 1000 ml of water, and the pH value is natural.

[0050] Operation: Put the above peptone and other raw materials into the water in the formula in any order, stir and dissolve, and then pack them into multiple test tubes with a diameter of 25mm and a length of 200mm. Each tube is filled with 5 ml, and placed in a medical sterilizer at 0.12-0.14 MPa. Sterilize for 20 minutes, the medium is sterilized

[0051] Inoculation: Open the purchased freeze-dried tube strain under aseptic conditions, then add 1 ml of the above sterilized medium to it, shake it to dissolve, and use it for later use. 5 ml of good bacteria culture medium, open the t...

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Abstract

The invention relates to a method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas. The method comprises steps of bacteria culture, seeding tank culture, fermentation tank culture and the like, and can produce hydroxysteroid dehydrogenase efficiently. The hydroxysteroid dehydrogenase has remarkable effects on crops in preventing disease, promoting growth and increasing yield. Furthermore, if being used as a novel fodder enzyme preparation in cultivation in stock farming, the hydroxysteroid dehydrogenase can effectively promote pigs and broiler chickens to grow, improves the laying rate of laying hens, reduces disease of beasts and birds, and has obvious effect in promoting growth. According to the method, viable bacteria in per milliliter of fermentation liquor is improved to be 30 billion from 0.1 billion, the enzyme activity in per gram of dry powder is improved to be 300 thousand from 1 thousand, so that the product cost is greatly lowered, and the method can be used for industrial production.

Description

technical field [0001] The invention relates to a method for efficiently producing hydroxysteroid dehydrogenase by Comamonas testosteroni. Background technique [0002] Any plant individual is a combination of the plant's own tissue cells, its internal and external enzymes, and microorganisms. That is to say, the enzymes and microorganisms themselves are part of the plant, and enzymes play a very important role in life activities. Enzymes can Catalyze various biochemical reactions under normal temperature and pressure, produce a variety of antibiotics and growth-promoting hormones, activate the soil to release a large amount of trace elements, increase the number of main roots and capillary roots of plants, broaden the pipeline of nutrient delivery, and allow crops to absorb in a reasonable balance Nutrients, promote crop growth, early maturity, increase income, enhance stress resistance, disease resistance, drought and flood resistance, etc. Without these enzymes, crops c...

Claims

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Application Information

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IPC IPC(8): C12N9/04C12R1/01
CPCC12N9/0006Y02A40/70
Inventor 张起凡
Owner 山东环亿生物科技有限公司
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