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A kind of preparation method of modified hydroxyapatite with amino acid grafted on the surface

A hydroxyapatite and surface grafting technology is applied in the field of preparation of hydroxyapatite materials to achieve the effects of increased adsorption, excellent biological compatibility and large adsorption capacity

Inactive Publication Date: 2015-12-30
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the synthesis of modified hydroxyapatite with strong protein adsorption and separation, however, there are few reports on the use of KH560 to treat HAP, and then use amino acids as ligands to modify hydroxyapatite to achieve specific adsorption effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Prepare 100ml of 9:1 (v / v) ethanol-water mixed solution, adjust the solution with glacial acetic acid to make the pH value 4, then accurately weigh 2.5% (w / v) KH560 2.5g with an electronic balance and mix thoroughly Stir for 30 minutes under a constant temperature vibration device to completely hydrolyze KH560, then add 1.5g of 1.5% (w / v) nanometer HAP powder into the flask, turn on magnetic stirring to make it evenly dispersed, and control the temperature of the water bath at 35°C for 0.5h. After the reaction, the product was fully washed with absolute ethanol several times and filtered with suction, and the obtained product was placed in a vacuum oven and vacuum-dried at 80° C. for 24 hours. Fully dissolve 0.3gL-serine in the prepared 0.2mol / L Na 2 HPO 4 and NaH 2 PO 4 Control the volume ratio in 100ml of the mixed buffer solution, adjust the pH value to 4 with glacial acetic acid, take 1g of modified HAP and add it to the flask to control the temperature of the wa...

Embodiment 2

[0025] Prepare 100ml of ethanol-water mixed solution of 9:1 (v / v), adjust the solution with glacial acetic acid to make the pH value 5, then accurately weigh 5.0% (w / v) KH5605g with an electronic balance and mix thoroughly at a constant temperature Stir under the vibration device for 30 minutes to completely hydrolyze KH560, then add 2.5g of 2.5% (w / v) nanometer HAP powder into the flask, turn on magnetic stirring to make it evenly dispersed, and control the temperature of the water bath at 45°C for 1 hour. After the reaction, the product was fully washed with absolute ethanol several times and filtered with suction, and the obtained product was placed in a vacuum oven and vacuum-dried at 80° C. for 24 hours. Fully dissolve 1g of leucine in the prepared 0.2mol / L Na 2 HPO 4 and NaH 2 PO 4 Control the volume ratio in 100ml of the mixed buffer solution, adjust the pH value to 5 with glacial acetic acid, take 1g of modified HAP and add it to the flask to control the temperature...

Embodiment 3

[0027] Prepare 100ml of 9:1 (v / v) ethanol-water mixed solution, adjust the solution with glacial acetic acid to make the pH value 6, and then use an electronic balance to accurately weigh 7.0% (w / v) KH5607g and mix thoroughly at a constant temperature Stir under the vibration device for 30 minutes to completely hydrolyze KH560, then add 3.5g of 3.5% (w / v) nano-HAP powder into the flask, turn on magnetic stirring to disperse it evenly, and control the temperature of the water bath at 55°C for 1.5 hours. After the reaction, the product was fully washed with absolute ethanol several times and filtered with suction, and the obtained product was placed in a vacuum oven and vacuum-dried at 80° C. for 24 hours. Fully dissolve 1.5g arginine in the prepared 0.2mol / L Na 2 HPO 4 and NaH 2 PO 4 Control the volume ratio in 100ml of the mixed buffer solution, adjust the pH value to 6 with glacial acetic acid, take 1g of modified HAP and add it to the flask to control the temperature of t...

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Abstract

The invention relates to a preparation method for modified hydroxyapatite with grafted amino acid on the surface. The method comprises the following steps: firstly, preparing a mixed solution of ethyl alcohol and water, adjusting the pH value, then adding KH560, stirring and shaking the solution, then adding nanoscale hydroxyapatite, reacting for 0.5-1.5 h and obtaining modified hydroxyapatite; secondly, drying and grinding the modified hydroxyapatite, placing the modified hydroxyapatite in a buffered solution with the pH value controlled to be within 6-8 and amino acid of 3.0-20 mg / mL, shaking the solution for 20-30 h at 35-65 DEG C and grating amino acid to obtain the modified hydroxyapatite with grafted amino acid on the surface. According to the invention, the preparing method is simple, and the obtained modified hydroxyapatite with grafted amino acid on the surface has excellent biological compatibility, adsorbs a considerable amount of protein and enzyme, and is wide in application prospects.

Description

technical field [0001] The invention belongs to the field of preparation of hydroxyapatite materials, in particular to a preparation method of modified hydroxyapatite with amino acid grafted on the surface. Background technique [0002] The molecular formula of hydroxyapatite (HAP) is Ca 10 (PO 4 ) 6 (OH) 2 , with a density of 3.16g / cm 3 , Brittle, the refractive index is 1.64 ~ 1.65. Slightly soluble in pure water, weakly alkaline, easily soluble in acid but hardly soluble in alkali. The crystal of hydroxyapatite belongs to the P63 / m space group, hexagonal crystal system, and the HAP present in hard tissues such as animal bones and teeth is needle-shaped due to its good biological compatibility, low density, chemical stability and compatibility with Similar in composition to human bone minerals, hydroxyapatite is widely used in biomedical engineering. The HAP synthesized in theoretical ratio is biocompatible, non-toxic, non-immunogenic, and has good biological activi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/04C02F1/28
Inventor 孙俊芬曹桢桢
Owner DONGHUA UNIV
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