Electric excitation cell culture method

Abstract

The present invention discloses an electric excitation cell culture method, wherein an electrostatic spinning electrode 1, a culture dish substrate 2, electric excitation cells 3, an electric stimulation module 4, and an electrode connection wire 5 are adopted. According to the present invention the structure is simple, and electric stimulation can be added based on three dimensional culture of the skeleton so as to improve culture quality of the electrically excitable cells.

Description

[0001] technical field [0002] Specifically, the invention relates to a method for culturing electro-excitable cells. Background technique [0003] In recent years, more and more attention has been paid to the research and development of cell culture, especially the problem of adapting to different application backgrounds and complex culture environments. The traditional cell culture is only static culture in the culture medium mechanically. For some cells that need electrical excitation, this culture method has received certain restrictions. [0004] Chinese application number CN201010243578.1, application publication number CN101892153A, this patent discloses a cell culture method of combined shear force-electric stimulation, the invention establishes a cell culture environment under force stimulation combined with electrical stimulation, which overcomes the existing There are technologies that cannot simulate the defect that cells in the body are in the environment of f...

AI Technical Summary

Problems solved by technology

Traditional cell culture is only mechanical static culture in culture medium. For some cells that need electrical excitation, this culture method has received certain restrictions.

[0004] Chinese application number CN201010243578.1, application publication number CN101892153A, which discloses a cell culture method of shear force-electrical combined stimulation, which establishes the The environment of cell culture under force stimulation combined with electric stimulation overcomes the defect that the existing technology cannot simulate the combined force and electric stimulation environment of cells in the body, and lays the foundation for promoting the functional culture of cells and tissues

However, the electrodes of this technology are only planar electrodes, which cannot adapt to the complex environment of cell culture.

[0005] Chinese application number CN 201010243576.2, application publication number CN101892152A, which discloses a stretch-electrical combined stimulation cell culture method, which overcomes the existing methods Can not simulate the defects of the physical microenvironment where the cells are in the stretching tension and electrical stimulation at the same time, the cell culture method under the stretching tension stimulation combined with electrical stimulation can be established, which can promote the development of cell and tissue culture methods and related tissue engineering reactors

However, this technology also uses traditional planar electrodes, which cannot provide a skeleton for cell culture, and there are certain limitations in its use.

[0006]In summary, although cell culture has been studied to a certain extent, there is no report on the method of electro-excitable cell culture in the literature.

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