Chinese chive seed antioxidative tripeptide, and preparation and application thereof

A technology for oxidizing tripeptides and leek seeds, which is applied in the fields of food and biomedicine, and can solve the problems of no reports of active peptides

Inactive Publication Date: 2014-06-18
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There has been no research on the chemical composition and efficacy of leek seeds abroad, and there are not many domestic researches

Method used

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  • Chinese chive seed antioxidative tripeptide, and preparation and application thereof
  • Chinese chive seed antioxidative tripeptide, and preparation and application thereof
  • Chinese chive seed antioxidative tripeptide, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The separation and purification of the antioxidant tripeptide in the present invention includes two steps of Sephadex G-50 gel filtration chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC).

[0028] Extract the leek seed extract: the leek seeds are crushed by a high-speed traditional Chinese medicine grinder, and 10% ( V / V ) soaked in acetic acid, ultrasonically assisted extraction for 20 min, stirred and extracted at room temperature for 6 h, centrifuged at 12000 rpm for 15 min, and the supernatant was freeze-dried and stored at -80 °C.

[0029] Sephadex G-50 Gel Filtration Chromatography: The leek seed extract freeze-dried powder was obtained according to the above method, dissolved in deionized water, and centrifuged at 12000 rpm for 15 min. The supernatant was filtered through a 0.22 μm pore size microfiltration membrane. Sephadex G-50 gel column (1.6cm×100cm) was equilibrated with deionized water, and the filtered sample was loaded...

Embodiment 1

[0034] The natural antioxidant tripeptide activity obtained in the implementation example 1 is studied:

[0035] DPPH free radical scavenging effect: 0.4 mL samples of different concentrations and an equal volume of 4% ( W / V ) DPPH absolute ethanol solution was mixed thoroughly, and kept at room temperature in the dark for 30 min, and the absorbance value was measured at 517 nm. Deionized water and reduced glutathione were used instead of samples as blank control and positive control, respectively. The DPPH free radical scavenging activity of the sample is calculated according to the following formula (1):

[0036] (1)

[0037] In the formula, A 0 : Absorbance value of blank control group; A s : Absorbance value of sample group

[0038]ABTS free radical scavenging effect: prepare 7 mmol / L ABTS with distilled water + solution and 2.45 mmol / L potassium persulfate solution (must be placed at room temperature for 16 h before use), ABTS + Mix with potassium persulfate s...

Embodiment 3

[0047] The protective effect of the antioxidant tripeptide of the present invention on hydrogen peroxide-induced normal liver cell damage:

[0048] The revived normal hepatocytes (LO2) were incubated with modified RPMI-1640 medium containing 10% fetal bovine serum, 2.0 mmol / L glutamate and 100 U / mL penicillin-streptomycin in a moist, 5% Carbon dioxide, adherent culture at 37 °C for 24-48 h. After digestion with trypsin at 37 °C, RPMI-1640 medium was added to stop the digestion, an appropriate amount of RPMI-1640 medium was added to blow and blow to fully disperse the cells, and the cells were inoculated into 96-well plates and continued to culture for 24 h, so that the final concentration of cells was 1.5×10 5 / hole. The cells were treated with different concentrations of tripeptide GSQ for 24 h, and then treated with 300 μmol / L hydrogen peroxide for 24 h, and the number of viable hepatocytes in each well was detected by MTT method.

[0049] From the test results, it ca...

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Abstract

The invention discloses an amino acid sequence of antioxidative tripeptide derived from Chinese chive seeds, a preparation method and application of the antioxidative tripeptide to biological medicines and food. The sequence of the antioxidative tripeptide is Gly-Ser-Gln (GSQ). In-vitro experiments show that the peptide has the effects of effectively removing free radicals such as DPPH, ABTS and superoxide anions and simultaneously protecting hydrogen peroxide-induced normal liver cells from damage. The antioxidative tripeptide has the characteristics of simple structure, high antioxidative activity and the like, can be used as a good substitute of a conventional artificially-synthesized antioxidant, and is significant for the development and application of novel antioxidative health products and medicaments.

Description

technical field [0001] The invention relates to a natural anti-oxidation tripeptide of chive seeds, a preparation method thereof and an application in the fields of food and biopharmaceuticals, belonging to the fields of food and biomedicine. Background technique [0002] my country is a large agricultural country with a wide variety of crops. However, due to the limitation of processing technology, the processing of crops is mostly in the primary stage, and there is still a relatively large amount of protein in the residues after processing, and most of them are mainly used for feed. Industrial or natural emissions not only waste resources, but also cause environmental pollution. The introduction of the concept of plant active peptides and the improvement of separation and detection technology have made people gradually pay attention to the deep processing of agricultural by-products rich in protein and obtain a variety of active peptides, such as casein phosphopeptides, blo...

Claims

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Application Information

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IPC IPC(8): C07K5/083A23L1/29A61K38/06A61P39/06A23L33/00
Inventor 洪晶陈涛涛汪少芸户佩俞世宏
Owner FUZHOU UNIV
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