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Tibet hazel agaricus bisporus strains and cultivation method of fruit bodies of Tibet hazel agaricus bisporus strains

A technology of Agaricus bisporus and fruiting bodies, applied in botany equipment and methods, horticulture, application, etc., can solve problems such as unfavorable cultivation and production of Agaricus bisporus, narrow genetic basis, and accelerated degradation of Agaricus bisporus species

Inactive Publication Date: 2014-06-25
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the source of the main strains of Agaricus bisporus in my country is relatively single, and the genetic basis is narrow, which may cause the reduction of the stress resistance and adaptability to adverse environments of Agaricus bisporus strains, and accelerate the degradation of Agaricus bisporus strains, so as not to Conducive to the cultivation and production of Agaricus bisporus
[0004] Most of the germplasm of Agaricus bisporus cultivation in China is imported from abroad. Most of them are white strains, and there are few brown varieties. Wild Agaricus bisporus cultivation resources are very scarce.

Method used

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  • Tibet hazel agaricus bisporus strains and cultivation method of fruit bodies of Tibet hazel agaricus bisporus strains
  • Tibet hazel agaricus bisporus strains and cultivation method of fruit bodies of Tibet hazel agaricus bisporus strains
  • Tibet hazel agaricus bisporus strains and cultivation method of fruit bodies of Tibet hazel agaricus bisporus strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1, the acquisition and classification identification of Hazel Agaricus bisporus 2094 in Tibet

[0061] The strain of the present invention (referred to as 2094) is obtained from fresh specimens of wild mushroom Agaricus bisporus collected from Nyingchi, Tibet Autonomous Region, and obtained through tissue separation, cultivation and domestication. The strain was classified and identified by observing the morphological characteristics of its fruiting bodies and mycelium, combined with nuclear rDNA-ITS sequencing.

[0062] 1. Morphological characteristics

[0063] 1. Morphological characteristics of mycelium

[0064] The hyphae of the bacterial strain 2094 of the present invention are dense and vigorous, white in the early stage, slightly light brown after a period of cultivation. Such as figure 1 , figure 2 shown.

[0065] 2. Morphological characteristics of fruiting bodies

[0066] The fruiting body morphology of bacterial strain 2094 of the present inven...

Embodiment 2

[0071]Example 2. Artificial cultivation of the fruiting bodies of Tibetan Hazel Agaricus bisporus 2094 (CGMCC No.8308)

[0072] 1. Cultivation process

[0073] The cultivation process of Tibetan Hazel Agaricus bisporus 2094 (CGMCC No.8308) is as follows: mother seed preparation → original seed preparation → cultivated seed preparation → inoculation and germination → soil covering → fruiting management → fruiting → harvesting.

[0074] (1) Preparation of the mother species: inoculate the sparse hyphae of Tibetan Hazel Agaricus bisporus 2094 (CGMCC No.8308) obtained from the tissue separation on the mother species medium, and the inoculation amount is 20 cm 2 Mycelium with an area size of 0.2cm×0.2cm is inserted into the surface area of ​​the mother seed medium, cultured at a certain temperature in the dark, until the mycelium is covered with the medium, and the obtained mycelium is used as the mother seed.

[0075] (2) Preparation of original seed: transfer the mother seed obt...

Embodiment 3

[0157] Example 3. Comparative analysis of Tibetan Hazel Agaricus bisporus 2094 (CGMCC No.8308) and existing As2796

[0158] In this example, from the three aspects of the basic traits of the strain, the nutritional components of the fruiting body, and the antioxidant capacity of the ethanol extract of the fruiting body, the Tibetan Hazel Agaricus bisporus 2094 (CGMCC No.8308) and the existing cultivars provided by the present invention are compared. Agaricus bisporus As2796 was comparatively analyzed.

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Abstract

The invention discloses Tibet hazel agaricus bisporus strains and a cultivation method of fruit bodies of the Tibet hazel agaricus bisporus strains. Agaricus bisporus is specifically Tibet hazel agaricus bisporus 2094, and is acquired by artificial domestication of wild strains from Nyingchi Prefecture of Tibet of China, and the preservation number in China General Microbiological Culture Collection Center is CGMCC No.8308. Experimental results show that the Tibet hazel agaricus bisporus 2094 (CGMCC No.8308) is high in fruit body protein content and coarse fiber content and slightly low in carbohydrate content, and fruit body ethanol extracts have high oxidation resistance. The main color of strain caps is hazel, the strains can rapidly sprout after being sown, and the time from inoculation to primordium generation is short. New varieties of the agaricus bisporus are developed, and development of the new varieties of the agaricus bisporus is of great significance in protecting germplasm resources of species, adjusting the variety structure of the agaricus bisporus, enriching a wild agaricus bisporus germplasm resource repository of China and promoting breeding and improvement of the agaricus bisporus strains.

Description

technical field [0001] The invention relates to a Tibetan Hazel Agaricus bisporus strain and a fruiting body cultivation method thereof. Background technique [0002] Agaricus bisporus (J.E.Lange) Imbach is the global edible fungus with the widest cultivation area and the largest output in the world today, and it is also one of the largest export-earning edible fungi in my country. With its rich nutrition, delicious taste and the function of improving the body's immunity, it is very suitable for the pursuit of modern people's food. Since Agaricus bisporus is the main cultivated and consumed edible fungus in developed countries in Europe and America, the development of Agaricus bisporus industry is also particularly important for promoting export trade. [0003] The artificial cultivation of Agaricus bisporus began in the era of Louis XIV in France, and was rapidly promoted in various countries in Europe and North America. It was introduced into China in the mid-1920s. At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 姚一建李斌斌蒋思萍魏铁铮徐爱国王晓亮张祖堂普布多吉
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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