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A New Method for Preparing High Purity Thymus

A thymus method and resin technology, applied in the field of peptide solid-phase synthesis, can solve problems such as difficulty in synthesizing peptides

Active Publication Date: 2016-09-07
郑州大明药物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are mainly solid-phase synthesis methods for chemically synthesizing thymosin. Although this method is relatively mature, it is very difficult to use this solid-phase synthesis method to synthesize polypeptides.

Method used

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  • A New Method for Preparing High Purity Thymus
  • A New Method for Preparing High Purity Thymus
  • A New Method for Preparing High Purity Thymus

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Experimental program
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Embodiment

[0076] The present invention prepares the new method of high-purity thymus method, and the detailed steps of this preparation method are as follows:

[0077] (1) Preparation of polypeptide fragment-Asn-Glu-Ala-Glu-Glu-Val-Val-Glu-Lys-Lys-Glu resin:

[0078] a. First add Fmoc-Asn(Trt)-wang resin resin into the container, then add dimethylformamide to swell, the swelling time is 30min; the ratio of the amount of the resin to dimethylformamide For 1g resin: 10mL dimethylformamide;

[0079]b, the resin after step a swelling is added in the mixed solution of piperidine and dimethylformamide to carry out deprotection, the mass ratio between piperidine and dimethylformamide in the mixed solution is 1:1, every gram of Fmoc -Asn(Trt)-wang resin was added to 10mL of the mixture, and after deprotection, it was washed 5 times with dimethylformamide;

[0080] c. Add the deprotected and washed resin in step b into the reactor, then add Fmoc-Glu(OtBu)-OH amino acid, condensation agent HATU...

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Abstract

The invention discloses a new method for preparing high-purity thymus. Firstly prepare polypeptide fragment one resin, polypeptide fragment two resin, polypeptide fragment three resin, polypeptide fragment four resin and polypeptide fragment five resin; split polypeptide fragment two, three, four and five resins to obtain each crude polypeptide fragment; will obtain The crude polypeptide fragments 2, 3, 4 and 5 were purified separately; the purified polypeptide fragments were connected to the polypeptide fragment one resin; the connected polypeptide fragment resins were acetylated to obtain thymus method new wang resin; cracking, Obtain the crude thymus method; purify the obtained thymus method twice; collect and purify the mobile phase containing thymus method, evaporate to dryness under reduced pressure, centrifuge to obtain the thymus method, and vacuum dry to obtain the thymus method product. Utilizing the method to prepare thymus farsin can effectively shorten the reaction time, improve the reaction yield and the quality of the final product; the prepared thymus farsin has a purity of more than 99%.

Description

technical field [0001] The invention relates to the technical field of polypeptide solid-phase synthesis, in particular to a new method for preparing high-purity thymus. Background technique [0002] Thymosin is a group of physiologically active polypeptides secreted by thymus tissue, which can promote lymphocyte transformation, enhance macrophage phagocytosis, and can be used to treat various immunodeficiency diseases. [0003] The new chemical structural formula of thymus method is: [0004] Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu- Glu-Ala-Glu-Asn-OH. [0005] In the treatment of chronic hepatitis B with thymusfasin alone, domestic and foreign studies have shown that the sustained response rate of thymusfasin alone in the treatment of chronic hepatitis B is about 37%, which is similar to that of interferon alone, but without the toxic side effects of interferon. [0006] The clinical research on the application ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/575C07K1/06C07K1/04
CPCC07K14/57581
Inventor 张宝国李沁沁王艳侨翟腾朱赞梅张亚惠苗海敏王娜
Owner 郑州大明药物科技有限公司