Breeding method of new halophilic crassostrea hongkongensis variety
A technology for Hong Kong oysters and new strains, which is applied in the field of seed production of new strains of Hong Kong oysters with high salt tolerance, can solve the problems of not cultivating new excellent varieties of Hong Kong oysters, etc. Effect
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[0019] a. Parental selection: On July 5, 2010, divers were hired to harvest 200 wild Hong Kong oysters of 2-3 years old in Hong Kong Deep Bay (water depth 5-10m) with an annual average salinity ≥ 24ppt, as a high-salt-tolerant parent group.
[0020] b. Parental maturation promotion: put 200 individuals into 10 scallop cages and place them in a pond with a salinity of 27-30ppt for ecological maturation promotion. After 15 days of ripening, 162 Hong Kong oyster gonads matured, and the remaining 38 dead individuals were discarded.
[0021] c. Progeny breeding: 162 Hong Kong oysters were used as a breeding group, and the group self-propagation was carried out in seawater with a salinity of 27-30ppt. The method of dissection was adopted, and after fertilization and hatching, larvae cultivation, juvenile growth, and juvenile growth, to obtain tolerance High-salt new strain F1. The specific growth and survival conditions are shown in Table 1 and Table 2.
[0022] d. Strain purifica...
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