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A kind of oscheius tipulae nematode cryopreservation solution and its preparation method and the application of the cryopreservation solution

A cryopreservation solution and nematode technology, applied in the field of cryopreservation solution, can solve the problem of Oscheiustipulae nematodes that cannot be frozen and preserved, and achieve the effect of ensuring sustainability

Inactive Publication Date: 2015-07-29
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention will solve the problem that conventional nematode glycerol freezing solution cannot freeze and preserve Oscheius tipulae nematode, and provide a kind of Oscheius tipulae nematode freezing solution and its preparation method and the application of the freezing solution

Method used

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specific Embodiment approach 1

[0018] Specific embodiment one: In this embodiment, a kind of Oscheius tipulae nematode cryopreservation solution is composed of 5% to 15% agar gel, 10% to 20% tetrahydrofuran and the balance as a buffer by volume percentage;

[0019] where KH in the buffer 2 PO 4 The concentration is 0.003g / mL, Na 2 HPO 4 The concentration of NaCl is 0.006g / mL, the concentration of NaCl is 0.004g / mL~0.006g / mL, MgSO 4 The concentration is 0.001g / mL;

[0020] The preparation method of described agar gel is as follows:

[0021] a. KH 2 PO 4 、Na 2 HPO 4 , NaCl and MgSO 4 Add distilled water to prepare a buffer solution, then put it into a high-pressure steam sterilizer, control the temperature at 120°C, and sterilize for 20 minutes. Among them, KH in the buffer solution 2 PO 4 The concentration is 0.003g / mL, Na 2 HPO 4 The concentration of NaCl is 0.006g / mL, the concentration of NaCl is 0.004g / mL~0.006g / mL, MgSO 4 The concentration is 0.001g / mL;

[0022] b. Add agar to the buffer s...

specific Embodiment approach 2

[0023] Specific embodiment two: the difference between this embodiment and specific embodiment one is: a kind of Oscheius tipulae nematode cryopreservation solution is composed of 8%~11% agar gel, 12%~18% tetrahydrofuran and balance by volume percentage composed of buffer. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0024] Embodiment 3: This embodiment is different from Embodiment 1 in that: a cryopreservation solution of Oscheius tipulae nematode is composed of 6% agar gel, 11% tetrahydrofuran and the balance as a buffer by volume percentage. Others are the same as in the first embodiment.

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Abstract

The invention relates to an Oscheius tipulae nematode cryopreservation solution and a preparation method and application thereof, relates to a cryopreservation solution and a preparation method and application thereof, and solves the problem that a conventional nematode glycerinum cryopreservation solution can not freeze and preserve Oscheius tipulae nematodes. The cryopreservation solution comprises agaropectin, tetrahydrofuran and a buffer solution. The preparation method comprises the following steps: 1, preparing the buffer solution; 2, preparing the agaropectin; and 3, preparing the cryopreservation solution. The cryopreservation solution is used for cryopreserving the Oscheius tipulae nematode. The Oscheius tipulae nematode cryopreserved by adopting the cryopreservation solution normally grows and propagates after being unfrozen and revived, and thus the continuity of indoor culture of the Oscheius tipulae nematode captured in field is ensured. The cryopreservation solution is used for cryopreserving the Oscheius tipulae nematode.

Description

technical field [0001] The invention relates to a cryopreservation liquid, a preparation method thereof and an application of the cryopreservation liquid. Background technique [0002] Oscheius tipulae is a small rod family nematode, which has a similar ecological niche and similar life form to Caenorhabditis elegans C.elegans. The inventor has captured Oscheius tipulae in the wild and successfully cultivated it indoors. Most nematodes can be stored for a long time at -80°C in the cryopreservation solution with glycerol as the main component, and can grow and reproduce normally after being revived at room temperature. However, Oscheius tipulae could not be recovered in glycerol cryopreservation solution, and further research found that glycerol was fatal to Oscheius tipulae. Therefore, the conventional glycerol cryopreservation solution is not suitable for the long-term preservation of Oscheius tipulae. Contents of the invention [0003] The present invention aims to so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 王云彪郭泰亮
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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