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Bacillus brevis, applications thereof, and method of fermentation to produce tyrothricin

A technology of brevibactin and brevibactin, which is applied in the field of brevibacillus and its application and fermentation to produce brevibactin, which can solve the problems of low brevibactin yield and long fermentation cycle, shorten the fermentation cycle and improve production capacity , the effect of increasing production

Active Publication Date: 2014-07-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new Brevibacillus and its application and fermentation method for producing gramactin in order to overcome the low yield of gramactin in the prior art and the defects of long fermentation cycle

Method used

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Examples

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Effect test

Embodiment 1

[0045] This example is used to illustrate the method of the present invention for producing gramicin by fermentation.

[0046] (1) Preparation of seed medium: mix beef extract, peptone, and NaCl to a constant volume. Based on the seed medium, the dosage of beef extract is 5g / L, the dosage of peptone is 10g / L, and the dosage of NaCl is 5g / L. Put 100mL of seed culture medium into a 300mL Erlenmeyer flask, sterilize in a sterilizing pot at 121°C for 0.5h, then cool down to 35°C for use.

[0047] (2) Seed culture: insert Bacillus brevis A into the seed medium of step (1), based on the seed medium per liter, the inoculum amount of Bacillus brevis is 9×10 9 spores. Then, the seed culture was carried out at 35°C, the initial pH value was 6.0, and the ventilation rate was 0.4 volume: (volume·min). After 72 hours of cultivation, the cultivation was stopped to obtain seed liquid.

[0048] (3) Preparation of fermentation medium: Mix casein peptone, NaCl, yeast extract powder, and so...

Embodiment 2

[0051] This example is used to illustrate the method of the present invention for producing gramicin by fermentation.

[0052] (1) Preparation of seed medium: mix beef extract, peptone, and NaCl to a constant volume. Based on the seed medium, the dosage of beef extract is 4g / L, the dosage of peptone is 12g / L, and the dosage of NaCl is 4g / L. Put 100mL of seed culture medium into a 300mL Erlenmeyer flask, sterilize in a sterilizing pot at 121°C for 0.5h, then cool down to 30°C for use.

[0053] (2) Seed culture: insert Bacillus brevis A into the seed medium of step (1), based on the seed medium per liter, the inoculum amount of Bacillus brevis is 6×10 8 spores. Then, the seed culture was carried out at 30°C, the initial pH value was 6.6, and the ventilation rate was 1.0 volume: (volume·min). After 65 hours of cultivation, the cultivation was stopped to obtain seed liquid.

[0054] (3) Preparation of fermentation medium: Mix casein peptone, NaCl, yeast extract powder, and so...

Embodiment 3

[0057] This example is used to illustrate the method of the present invention for producing gramicin by fermentation.

[0058] (1) Preparation of seed medium: mix beef extract, peptone, and NaCl to a constant volume. Based on the seed medium, the amount of beef extract is 6g / L, the amount of peptone is 8g / L, and the amount of NaCl is 6g / L. Put 100mL of seed culture medium into a 300mL Erlenmeyer flask, sterilize in a sterilizing pot at 121°C for 0.5h, then cool down to 40°C for use.

[0059] (2) Seed culture: insert Bacillus brevis A into the seed medium of step (1), based on the seed medium per liter, the inoculum amount of Bacillus brevis is 9×10 10 spores. Then, the seed culture was carried out under the conditions of 40°C, initial pH value of 6.8, and ventilation rate of 1.5 vol:(vol min). After 75 hours of cultivation, the cultivation was stopped to obtain seed liquid.

[0060] (3) Preparation of fermentation medium: mix casein peptone, NaCl, yeast extract powder, an...

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Abstract

The invention discloses bacillus brevis. The bacillus brevis is characterized in that the accession number of the bacillus brevis is CGMCC No.6284. The invention also discloses applications of the bacillus brevis in fermentation to produce tyrothricin. The invention also discloses a method of fermentation to produce the tyrothricin. The method is characterized by including inoculating the bacillus brevis into a fermentation culture medium under conditions of producing the tyrothricin, and fermenting to obtain a fermentation broth. The bacillus brevis is used for fermentation to produce the tyrothricin, and is capable of increasing the output of the tyrothricin and shortening the fermentation period, thus boosting the production capability of the tyrothricin. The bacillus brevis and the method can provide an effective and feasible technical scheme for large-scale production of the tyrothricin.

Description

technical field [0001] The invention relates to a Bacillus brevis, an application of the Bacillus brevis in fermenting and producing breviscin, and a method for producing breviscin by using the Bacillus brevis in fermentation. Background technique [0002] Tyrothricin, also known as mixed gramicidin, is divided into two types: linear gramicidin and cyclic tyrocidine, which are all extracted from the fermentation broth of Bacillus brevis of. [0003] Brevicins are an important class of antibiotic substances. For Gram-positive bacteria and some Gram-negative bacteria, both linear gramicidin and cyclic gramicidin have good killing effects. Their antibacterial properties are due to their ability to form bacterial cell walls The permeability of the bacteria is enhanced, so that the activities of cell metabolism cannot be carried out normally, and the bacteria are inhibited or even die. Utilizing the antibacterial properties of gramicillin, people generally make it into ointmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00C12R1/08
Inventor 郭慧媛任发政姜鹭张宇光
Owner CHINA AGRI UNIV
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