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A method of applying turbidity detection to the extraction of medicinal materials
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An extraction method and turbidity technology are applied in the field of extraction of Chinese medicinal materials to achieve the effects of improving quality stability, convenient operation, and accurate and reliable results.
Active Publication Date: 2018-10-16
TIANJIN TASLY MORDEN TCM RESOURCES
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[0006] Recently, it has been reported in the literature that turbidity is used in the field of traditional Chinese medicine production, most of which are used to indicate the clarity of traditional Chinese medicine oral liquid, but there is no report on the effect of controlling alcohol precipitation
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Embodiment 1
[0058] Step 1, Radix Astragali is extracted with water;
[0059] Step 2, adding alcohol to the extract to precipitate, and separating the supernatant after standing for a certain period of time;
[0060] Step 3, measure the turbidity of the alcohol precipitation supernatant with a turbidimeter, and when the turbidity is controlled between 0-600ntu, to determine the separation end point of the supernatant;
[0061] Step 4, the supernatant of alcohol precipitation is concentrated to obtain Radix Astragali extract.
Embodiment 2
[0063] Step 1: Astragalus is decocted and extracted with 10 times the amount of water, the extraction time is 2 hours, a total of 2 extractions, the extracts are combined, and the extracts are concentrated by vacuum distillation until the concentration reaches 1 / 10 of the volume of the extracts;
[0065] Step 3, use a centrifuge to separate the alcohol-precipitated liquid until the turbidity of the supernatant after centrifugation is <600ntu, and collect the supernatant;
[0066] Step 4, the supernatant is concentrated to obtain the astragalus extract extract.
Embodiment 3
[0068] The extraction method of Astragalus medicinal material, the steps are as follows:
[0069] Step 1: Astragalus is decocted and extracted with 10 times the amount of water, the extraction time is 2 hours, a total of 5 extractions are made, the extracts are combined, and the extract is concentrated by vacuum distillation until it reaches 1 / 10 of the volume of the extract;
[0071] Step 3. After standing still, take an appropriate amount of supernatant, and measure its turbidity with a turbidimeter. When the turbidity of the supernatant is <200ntu, absorb the supernatant, and use a centrifuge to separate the supernatant from the remaining alcohol precipitation liquid. Merge the supernatant; when the turbidity of the supernatant is <200ntu, directly use a centrifuge to separate the supernatant;
[0072] Step 4, the supernatant is concentrat...
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Abstract
The invention relates to a method for extracting a traditional Chinese medicinal material, and especially relates to a method for extracting a medicinal material difficult to separate through traditional Chinese medicinealcoholprecipitation. The extraction method comprises the following steps: 1, adding water to the traditional Chinese medicinal material for extraction; 2, adding alcohol to the above obtained extract liquid, allowing the obtained mixture to stand for a certain time, and separating the obtained supernatant; 3, measuring the turbidity of the alcoholprecipitation supernatant by using a turbidity meter to determine the separation end point of the supernatant; and 4, concentrating the alcohol precipitation supernatant to obtain a traditional Chinese medicinal extract.
Description
technical field [0001] The invention relates to an extraction method of traditional Chinese medicinal materials, in particular to an extraction method of medicinal materials difficult to separate by alcohol precipitation of traditional Chinese medicine. Background technique [0002] Water extraction and alcohol precipitation process, as a commonly used refining and impurity removal method, is widely used in the production process of traditional Chinese medicine extracts. In the traditional alcohol precipitation method, after adding ethanol to the medicinal solution, in order to allow the insoluble precipitate to settle fully, the method of standing still is generally adopted, and then the supernatant is absorbed after the supernatant is clarified; for those that are difficult to separate by sedimentation, filtration or Insoluble impurities are removed by centrifugation. The clarification of the supernatant or centrifugate is generally judged subjectively by visual observati...
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