Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining chemical compositions in Shenqi Fuzheng injection and application thereof

A technology for Shenqi Fuzheng and chemical components, which is applied in the field of evaluating the chemical components of traditional Chinese medicines, and can solve the problems of difficulty in evaluating the active components of Shenqi Fuzheng injection, unfavorable product safety and effectiveness, and the like.

Active Publication Date: 2014-07-09
LI MIN PHARM FAB OF LIVZON PHARM GRP +1
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the current quality standard of Shenqi Fuzheng Injection, the original quality standard fingerprint only focused on the S peak (verbacosin) and the more polar water-soluble components, as well as the Astragalus A under the content determination item. glycoside content, it is difficult to evaluate the active ingredients in Shenqi Fuzheng Injection, and its negatively related ingredients, which is not conducive to the standard control of product safety and effectiveness

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining chemical compositions in Shenqi Fuzheng injection and application thereof
  • Method for determining chemical compositions in Shenqi Fuzheng injection and application thereof
  • Method for determining chemical compositions in Shenqi Fuzheng injection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: the screening of HSCCC separation condition

[0086] 1. Experimental method

[0087] Screening of HSCCC solvent system: Take Shenqi Fuzheng Injection SFI and filter it through a 0.45 μm filter membrane, then inject 75 μl directly with HPLC-DAD-ELSD, and measure the common peak area A1; Proportions of ethyl acetate-ethanol-water and ethyl acetate-n-butanol-water) were fully shaken, and after standing still, take the water phase (lower layer solution) and rotary evaporate to recover the solvent to dryness, ultrasonically dissolve with water and set the volume to 5ml, After filtering through a 0.45 μm filter membrane, inject 75 μl directly, measure the common peak area A2, and calculate the partition coefficient K value.

[0088] Screening of HSCCC collection time: Prepare a screened solvent system, separate by sample injection, and analyze each part of the separated sample by HPLC-DAD-ELSD sample injection, and finally determine the HSCCC separation metho...

Embodiment 2

[0105] Embodiment 2: the preparation of difference sample

[0106] 1. Experimental method:

[0107] (1) Repeatability investigation of the HSCCC method: adopt the separation conditions of HSCCC screened in Example 1 (conditions are as follows a) to collect four parts of the liquid, repeat the operation seven times, and perform HPLC-DAD-ELSD fingerprint analysis ( The conditions are as follows b), and the repeatability of the HSCCC method was investigated with the Chinese medicine chromatographic fingerprint similarity evaluation system (version 2009).

[0108] A. Solvent system: ethyl acetate-n-butanol-water 4:1:5, after standing still, take the upper phase as the stationary phase, and the lower phase as the mobile phase; sample volume: 250ml SFI concentrated to 20ml, rotary evaporated to dryness Dissolve in 5ml solvent system for later use; flow rate: 2ml / min; six-way valve connection: forward to forward rotation; rotational speed: 900rpm; detection wavelength: 254nm; sam...

Embodiment 3

[0160] The mensuration of the fingerprint spectrum of embodiment 3 difference samples

[0161] According to the same conditions and operations as the HPLC-DAD-ELSD spectrum measurement in embodiment 2, the difference samples 1 to 9 prepared in embodiment 2 are respectively subjected to fingerprint spectrum measurement, and the obtained fingerprint spectrum and peak area are shown in Figure 8 and Table 16.

[0162] Table 16 Fingerprint characteristics of difference samples 1-9

[0163]

[0164] Continuation of Table 16:

[0165]

[0166] The inventor also performed a cluster analysis on the above-mentioned differential samples: according to the above-mentioned HPLC-DAD-ELSD fingerprints, the peak areas of the 23 common peaks of the differential samples 1-9 were obtained respectively, and were imported into SPSS16.0 for clustering For analysis, the clustering method uses Nearest neighbor, and the distance calculation method uses Euclidean distance. The results show t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A provided method for determining chemical compositions in Shenqi Fuzheng injection comprises the following steps: (1) preparing a test sample of Shenqi Fuzheng injection; (2) preparing a differentiated sample; (3) determining a fingerprint of the differentiated sample; (4) determining the drug effect of the differentiated sample; and (5) performing spectrum-activity relationship analysis to evaluate the drug effect activity of common peaks. By employing the method disclosed by the invention, the inventor discovers that No. 9 peak, No. 11 peak, No. 12 peak, No. 15 peak and No. 20 peak in the fingerprint of Shenqi Fuzheng injection are active compositions, and No. 5 peak and / or No. 13 peak are negative correlated compositions, and the method is beneficial for controlling traditional Chinese medicine quality, safety and effectiveness.

Description

technical field [0001] The invention relates to a method for evaluating chemical components of traditional Chinese medicines, in particular to a method for evaluating chemical components of Shenqi Fuzheng Injection and the application of the method. Background technique [0002] Codonopsis (Radix Codonopsis) has mild medicinal properties, wide uses, and various pharmacological activities. It not only has an effect on digestion and cardiovascular system, but also regulates the function of the immune system, enhances the body's resistance, and has little toxicity. Astragalus (Radix Astragalus) is the main drug for invigorating qi, which can significantly increase the phagocytic function of macrophages. When used in combination with Codonopsis pilosula, it can enhance the macrophages' ability to phagocytose harmful substances. [0003] Shenqi Fuzheng Injection is a pure Chinese medicine preparation made by extracting Codonopsis pilosula and Astragalus membranaceus. Codonopsis ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 苏薇薇王锦旭童欣曹晖李沛波彭维王永刚
Owner LI MIN PHARM FAB OF LIVZON PHARM GRP