Unlock instant, AI-driven research and patent intelligence for your innovation.

Method of isolating and detecting related components of Shenqi Fuzheng injection

A technology of Shenqi Fuzheng and injection, applied in the fields of analytical chemistry and natural product chemistry, to achieve the effects of fast reaction, simple operation and stable repeatability

Active Publication Date: 2019-03-08
LI MIN PHARM FAB OF LIVZON PHARM GRP
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, although Shenqi Fuzheng Injection has established a variety of quality control and testing methods; most of them only detect the index components of Codonopsis pilosula or Astragalus membranaceus, and lack the components of Codonopsis pilosula and Astragalus membranaceus, as well as flavonoids and saponins in Astragalus membranaceus. Rapid, efficient, and comprehensive separation and evaluation methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of isolating and detecting related components of Shenqi Fuzheng injection
  • Method of isolating and detecting related components of Shenqi Fuzheng injection
  • Method of isolating and detecting related components of Shenqi Fuzheng injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Codonopsis pilosula extract (including alcohol) total ion current chromatogram (TIC) and extracted ion diagram (EIC)

[0076] Take about 10g of Codonopsis pilosula and extract with 100ml, 80ml, 70ml of water, heating for 1h, 1h, 0.5h. Combine the three extracts and concentrate them with 75% and 85% ethanol and two alcohol precipitation to obtain.

[0077] Weigh about 5 g of polyamide, degas with 95% ethanol, and pack it into a column (the column height is about 10 cm, and the inner diameter is 1 cm). After activation with 40 ml of 5% sodium hydroxide and 80 ml of 10% glacial acetic acid in turn, wash with water for injection until it is neutral. Precisely measure 25ml of the above-prepared Codonopsis pilosula extract and load the sample onto the activated polyamide chromatography column, and eluate with 80ml of water, 80ml of 20% ethanol, and 80ml of 60% ethanol. Receive the 60% ethanol eluate, evaporate to dryness in a water bath, and dissolve the residue with...

Embodiment 2

[0090] Example 2 Mass spectrum total ion chromatogram (TIC) and extracted ion diagram (EIC) of Astragalus extract

[0091] Take about 10g of Astragalus medicinal material, and extract with 100ml, 80ml, 70ml of water, heating for 1h, 1h, 0.5h. Combine the three extracts and concentrate them with 75% and 85% ethanol and two alcohol precipitation to obtain.

[0092] Weigh about 5 g of polyamide, degas with 95% ethanol, and pack it into a column (the column height is about 10 cm, and the inner diameter is 1 cm). After activation with 40 ml of 5% sodium hydroxide and 80 ml of 10% glacial acetic acid in turn, wash with water for injection until it is neutral. Precisely measure 25ml of the above-prepared astragalus extract and apply it to the activated polyamide chromatography column, eluting with 80ml of water, 80ml of 20% ethanol and 80ml of 60% ethanol. Receive the 60% ethanol eluate, evaporate to dryness in a water bath, and dissolve the residue with 60% ethanol and dilute to 25ml...

Embodiment 3

[0096] Example 3 TIC and EIC profiles of representative components of Astragalus and Codonopsis

[0097] Accurately weigh about 5 mg of Codonopsis, 12.5 mg of Isoflavone Glucoside and 10 mg of Astragaloside IV in water, 30% methanol, and methanol to prepare 1 mg / ml, 5 μg / ml, and 0.01 mg / ml solutions. Component analysis using LC-MS:

[0098] Chromatographic conditions, mass spectrometry conditions and determination methods: the same as in Example 1;

[0099] See the maps separately image 3 , Figure 4 with Figure 5 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
specific surface areaaaaaaaaaaa
Login to View More

Abstract

The invention provides a method of quickly isolating and evaluating related components of Shenqi Fuzheng injection. The method includes separating Shenqi Fuzheng injection by polyamide columns, performing LC-MS (liquid chromatography / mass spectrometry) detection, and using precise molecular weights of 441.1678, 441.2007, 471.2083, 491.1195, 507.1508, 633.2553, 829.4591, 871.4697, 913.4650 and 991.5119 to extract an EIC (extracted ion chromatogram) in a TIC (total ions chromatogram) so as to determine distributions of Radix Codonopsis and Radix Astragali index components in a preparation.

Description

Technical field [0001] The invention belongs to the technical field of analytical chemistry and natural product chemistry, and specifically relates to a method for separating and detecting related components of Shenqi Fuzheng injection. Background technique [0002] Radix Codonopsis has a mild medicinal property, a wide range of uses, and has various pharmacological activities. It not only has an effect on the digestive and cardiovascular system, but also regulates the immune system function and enhances the body's resistance. It has little toxicity. Radix Astragalus (Radix Astragalus) is the main medicine for invigorating qi, which can significantly increase the phagocytic function of macrophages. Combined with Codonopsis pilosula, it can enhance the phagocytosis of harmful substances by macrophages. [0003] Shenqi Fuzheng Injection is the exclusive Chinese medicine variety of Limin Pharmaceutical Factory. It is a pure Chinese medicine preparation made from Codonopsis pilosula an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 汪冰冰刘学华黄文华冯丽洁江晓漫
Owner LI MIN PHARM FAB OF LIVZON PHARM GRP