A method for separation and detection of related components of Shenqi Fuzheng Injection
A technology of Shenqi Fuzheng and injection, applied in the fields of analytical chemistry and natural product chemistry, to achieve the effects of fast reaction, rapid separation and detection, and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0075] Example 1 Total ion chromatogram (TIC) and extracted ion chromatogram (EIC) of Codonopsis pilosula extract (containing alcohol)
[0076] Take about 10g of Codonopsis pilosula, heat and extract with 100ml, 80ml, and 70ml of water for 1h, 1h, and 0.5h respectively. The three extracts were combined, concentrated and then alcohol-precipitated twice with 75% and 85% ethanol respectively to obtain the product.
[0077] Weigh about 5 g of polyamide, degas it with 95% ethanol and then wet pack it into a column (column height about 10 cm, inner diameter 1 cm). After being activated with 40 ml of 5% sodium hydroxide and 80 ml of 10% glacial acetic acid in sequence, it was washed with water for injection until neutral. Precisely measure 25ml of the above-prepared Codonopsis pilosula extract to the activated polyamide chromatography column, and then elute with 80ml of water, 80ml of 20% ethanol and 80ml of 60% ethanol. Receive 60% ethanol eluate, evaporate to dryness in a water...
Embodiment 2
[0087] Example 2 Mass Spectrometry Total Ion Chromatogram (TIC) and Extracted Ion Chart (EIC) of Radix Astragalus Extract
[0088] Take about 10g of Radix Astragali, heat and extract with 100ml, 80ml, and 70ml of water for 1h, 1h, and 0.5h respectively. The three extracts were combined, concentrated and then alcohol-precipitated twice with 75% and 85% ethanol respectively to obtain the product.
[0089] Weigh about 5 g of polyamide, degas it with 95% ethanol and then wet pack it into a column (column height about 10 cm, inner diameter 1 cm). After being activated with 40 ml of 5% sodium hydroxide and 80 ml of 10% glacial acetic acid in sequence, it was washed with water for injection until neutral. Precisely measure 25ml of the above-prepared astragalus extract and load it onto the activated polyamide chromatography column, and elute with 80ml of water, 80ml of 20% ethanol and 80ml of 60% ethanol in sequence. Receive 60% ethanol eluate, evaporate to dryness in a water bath...
Embodiment 3
[0093] Example 3 TIC and EIC spectra of representative components of Radix Astragali and Codonopsis Radix
[0094] Precisely weigh about 5 mg of tangshenoside, 12.5 mg of calycosin glucoside, and 10 mg of astragaloside in water, 30% methanol, and methanol to prepare 1 mg / ml, 5 μg / ml, and 0.01 mg / ml solutions. Component analysis by LC-MS:
[0095] Chromatography conditions, mass spectrometry conditions and determination method: with embodiment 1;
[0096] Spectrum see respectively image 3 , Figure 4 and Figure 5 .
PUM
| Property | Measurement | Unit |
|---|---|---|
| specific surface area | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More