Method for evaluating chemical components of Shenqi Fuzheng Injection and its application
A technology of Shenqi Fuzheng and chemical composition, which is applied in the field of evaluation of chemical composition of traditional Chinese medicine, and can solve problems such as difficult evaluation of active ingredients of Shenqi Fuzheng injection, unfavorable product safety and effectiveness, etc.
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Embodiment 1
[0086] Embodiment 1: the screening of HSCCC separation condition
[0087] 1. Experimental method
[0088] Screening of HSCCC solvent system: Take Shenqi Fuzheng Injection SFI and filter it through a 0.45 μm filter membrane, then inject 75 μl directly with HPLC-DAD-ELSD, and measure the common peak area A1; Fully oscillate in different proportions of ethyl acetate-ethanol-water and ethyl acetate-n-butanol-water), after standing still, take the water phase (lower layer solution) and rotary evaporate to recover the solvent to dryness, ultrasonically dissolve with water and set the volume to 5ml , after being filtered by a 0.45 μm filter membrane, inject 75 μl of the sample directly, measure the common peak area A2, and calculate the partition coefficient K value.
[0089] Screening of HSCCC collection time: Prepare a screened solvent system, separate by sample injection, and analyze each part of the separated sample by HPLC-DAD-ELSD sample injection, and finally determine the ...
Embodiment 2
[0106] Embodiment 2: the preparation of difference sample
[0107] 1. Experimental method:
[0108] (1) The repeatability investigation of HSCCC method: adopt the separation condition (condition as following a) of the HSCCC screened in embodiment 1 to collect the liquid of four parts, repeat operation seven times, carry out HPLC-DAD-ELSD fingerprint spectrum analysis ( The conditions are as follows b), and the repeatability of the HSCCC method was investigated with the Chinese medicine chromatographic fingerprint similarity evaluation system (2009 edition).
[0109] A. Solvent system: ethyl acetate-n-butanol-water 4:1:5, after standing still, take the upper phase as the stationary phase, and the lower phase as the mobile phase; sample volume: 250ml SFI concentrated to 20ml, rotary steamed to After drying, dissolve it in 5ml solvent system for later use; flow rate: 2ml / min; six-way valve connection: forward to forward rotation; speed: 900rpm; detection wavelength: 254nm; sam...
Embodiment 3
[0161] The mensuration of the fingerprint spectrum of embodiment 3 difference samples
[0162] According to the same conditions and operations as the HPLC-DAD-ELSD spectrum measurement in Example 2, the difference samples 1 to 9 prepared in Example 2 were respectively subjected to fingerprint spectrum measurement, and the obtained fingerprint spectrum and peak area are shown in Figure 8 and Table 16.
[0163] Table 16 Fingerprint characteristics of difference samples 1-9
[0164]
[0165] Continuation of Table 16:
[0166]
[0167] The inventor also performed a cluster analysis on the above-mentioned differential samples: according to the above-mentioned HPLC-DAD-ELSD fingerprints, the peak areas of the 23 common peaks of the differential samples 1 to 9 were obtained respectively, and were imported into SPSS 16.0 for cluster analysis , the clustering method uses the nearest neighbor method, and the distance calculation method uses Euclidean distance. The results sh...
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