Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection method of characteristic spectrum of Xiasangju preparation

A technology of characteristic map and detection method, which is applied in the field of detection of characteristic map of Xiasangju preparations, can solve the problems that it is difficult to fully characterize the physical and chemical characteristics of Xiasangju preparations, and the quality control method of compound Xiasangju preparations needs to be further improved, so as to reduce the possibility of manual processing , Comprehensive monitoring of product quality, good reproducibility

Active Publication Date: 2016-04-13
GUANGZHOU XINGQUN PHARMA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above methods for identification or content determination of individual components are difficult to fully characterize the physical and chemical characteristics of Xiasangju preparations. Therefore, the quality control methods for compound Xiasangju preparations need to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of characteristic spectrum of Xiasangju preparation
  • Detection method of characteristic spectrum of Xiasangju preparation
  • Detection method of characteristic spectrum of Xiasangju preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Construction of Xia Sangju Granule Characteristic Map

[0027] 1. Instruments, reagents and materials

[0028] 1.1 Instrument

[0029] KQ-100B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.);

[0030] BPZ11D electronic analytical balance (Sartorius company);

[0031] Waters2695-2996 high-performance liquid chromatography system, Empower workstation, including quaternary gradient pump, automatic sampler (Waters).

[0032] 1.2 Reagents

[0033] Glacial acetic acid (chromatographically pure, Beijing Chemical Plant);

[0034] Methanol (chromatographically pure, TEDIA company);

[0035] Acetonitrile (chromatographically pure, TEDIA company);

[0036] The water is wahaha pure water.

[0037] 1.3 Reference substance

[0038] Chlorogenic acid, isorosmarinic acid, rosmarinic acid, and mongoside were provided by Guangzhou Baiyunshan Xingqun (Pharmaceutical) Co., Ltd.

[0039] 1.4 Materials

[0040] 1.4.1 Xiasangju Granules produced by Guangzhou Ba...

Embodiment 2

[0067] A detection method for the characteristic spectrum of Xiasangju oral liquid, which is determined by high-performance liquid chromatography, comprising the steps of:

[0068] (1) Preparation of reference substance solution: Accurately weigh chlorogenic acid, isorosamarinic acid, rosmarinic acid and mongoside reference substances respectively, dissolve them in methanol, and prepare the concentrations of 0.1100, 0.1512, 0.1730 and 0.1730 respectively. 0.1478 μg / μl.

[0069] (2) Preparation of the test solution: Accurately weigh 2g of Xiasangju Oral Liquid, add 60% methanol solution to dissolve, set the volume to 10mL, ultrasonicate for 30min (power 240W, frequency 33kHz), let cool, make up to the scale , filtered, and the filtrate was taken as the test solution;

[0070] (3) Accurately draw need testing solution and reference substance solution respectively, inject high-performance liquid chromatograph and measure, and gained feature collection contains such as figure 1 ...

Embodiment 3

[0073] A method for detecting the characteristic spectrum of sugar-containing granules of Xiasangju, which is determined by high-performance liquid chromatography, comprising the following steps:

[0074] (1) Preparation of reference substance solution: Accurately weigh chlorogenic acid, isorosamarinic acid, rosmarinic acid and mongoside reference substances, dissolve them in methanol, and prepare the concentrations of 0.2520, 0.2920, 0.1610 and 0.2615 μg / μl.

[0075] (2) Preparation of the test solution: Accurately weigh 2g of 46 batches of Xiasangju sugar-containing granules, dissolve them in methanol solution with a volume fraction of 60%, set the volume to 20mL, and ultrasonicate for 30min (power 260W, frequency 35kHz), Let it cool, make up to the mark, filter, and take the continued filtrate as the test solution;

[0076] (3) Accurately draw need testing solution and reference substance solution respectively, inject high-performance liquid chromatograph and measure, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a detection method for a Xiasangju preparation characteristic spectrum, comprising the following steps: preparing a reference substance liquid: respectively weighing chlorogenic acid, rosmarinic acid glycoside, rosmarinic acid and linarin reference substances, and dissolving with methyl alcohol to be taken as the reference substance solution; preparing a to-be-tested substance liquid: weighing a Xiasangju preparation, putting into a volumetric flask, adding a methanol solution with volume fraction of 60-100% and dissolving, carrying out ultrasonic treatment, chilling, supplementing until reaching the scale, filtering, and taking a subsequent filtrate to be taken as the to-be-tested substance solution; respectively sucking the to-be-tested substance solution and the reference substance solution, and injecting into a high performance liquid chromatograph (HPLC) to be detected. According to the invention, chlorogenic acid, rosmarinic acid glycoside, rosmarinic acid and linarin are taken as reference indexes to obtain a detection method for a Xiasangju preparation characteristic spectrum, and optimal parameters for preparation of the to-be-tested substance solution and the reference substance solution and optimal parameters of the chromatographic conditions of the HPLC are determined. The method has the advantages of being simple and convenient to operate, stable in result and good in reproducibility.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicine preparations, in particular to a detection method for the characteristic spectrum of Xiasangju preparations. Background technique [0002] Xiasangju preparation is made from traditional Chinese medicinal materials Prunella vulgaris, mulberry leaves, and wild chrysanthemums, which are extracted with water and made according to conventional pharmaceutical processes. Anemopyretic cold, conjunctival congestion, headache, high blood pressure, dizziness, tinnitus, sore throat, treating sores and swollen toxins. Xia Sangju preparation Xia Sangju granule is included in the fifteenth volume of Chinese medicine prescription preparations of the Ministry of Health of the People's Republic of China. It is a popular traditional Chinese medicine preparation. Now Xia Sangju granule, Xia Sangju oral liquid, Xia Sangju beverage and other dosage forms are on the market. [0003] The qu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 姚江雄许招懂方铁铮黎志坚林丽美蒋莉娟
Owner GUANGZHOU XINGQUN PHARMA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com