Group b streptococcus quantum dot immunochromatography detection reagent card and preparation method thereof

Abstract

The invention discloses a group B streptococcus quantum dot immunochromatographic detection reagent card and a preparation method for the same, and aims to provide a simple, convenient and high-sensitivity group B streptococcus quantum dot immunochromatographic detection reagent card by using which a reliable detection result can be obtained. The detection reagent card comprises a box body (1) with a box cover, wherein test paper is arranged in the box body (1), and comprises a bottom plate (1); a sample pad (2), an anti-group B streptococcus quantum dot marker pad (3), a coating membrane (4) and an absorbent pad (5) are sequentially connected with the bottom plate (1); a group B streptococcus antibody detection area T line and a goat anti-rabbit polyclonal antibody quality control area C line are arranged in parallel on the coating membrane (4). The group B streptococcus quantum dot immunochromatographic detection reagent card and the preparation method for the same belong to the field of fluorescent quantum dot labeled immunological detection.

Description

technical field [0001] The invention discloses a detection reagent card, specifically, a detection reagent card for detecting group B streptococcus in the cervix or vagina of pregnant women. The invention also discloses a preparation method of the detection reagent card, which belongs to fluorescent quantum dot labeling field of immunoassays. Background technique [0002] Group B streptococcus (GroupB Streptococcus, referred to as GBS) can cause neonatal sepsis, pneumonia, meningitis, and even death. Neonates who survive infection may also have severe neurological sequelae, including hydrocephalus, intellectual disability, microcephaly, and deafness. At the same time, Group B Streptococcus can also cause infections in pregnant women, premature birth, fetal dysplasia (low birth weight children), premature rupture of membranes and late miscarriage. Therefore, the detection of group B streptococci in pregnant women is very important and necessary. At present, the detection o...

AI Technical Summary

Problems solved by technology

[0003] 1. The traditional bacterial culture detection method, the detection result of this method is more accurate, but the detection time is longer, generally it takes 2-3 days to get the result, and the efficiency is low;

[0004] 2. PCR detection method. This detection method is simple, fast and has high sensitivity. However, when detecting, it is easy to cause cross-amplification reactions, thereby causing false positive results. Therefore, professional technicians and special instruments and equipment are required. Therefore, this The scope of application of the method is very limited

[0005] 3. Colloidal gold detection method, this method is simple to operate, but the sensitivity is low, especially if the amount of infection is small, there may be missed detection or false detection, which will bring serious harm to the prenatal and postnatal care of the fetus

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