A method for rapid purification and rejuvenation of local varieties of taro
A technology for purifying and rejuvenating varieties, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as time-consuming, and achieve the effects of high quality, high yield and fast purification.
Inactive Publication Date: 2016-04-27
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology
The traditional method of purification and rejuvenation of varieties takes 3-4 years, which takes a long time
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[0033] The present invention will be further described below by obtaining a purified and rejuvenated line based on the Chinese geographical landmark agricultural product, Jingjiang sand taro, using tissue culture technology.
[0034] 1 Materials and methods
[0035] 1.1 Test material
[0036] Jingjiang fragrant sand taro farm varieties harvested in the field.
[0037] 1.2 Method
[0038] 1.2.1 Cultivation of virus-free tissue culture strains of taro
[0039] (1) Row formation of tissue-cultured seedlings: select well-preserved taro seedlings without rotten spots, and test them with 0.2% K 2 MnO 4 After sterilizing for 5 minutes, arrange them neatly in the turnover box, cover 2 / 3 of the whole taro block with sand, and place it in the light incubator to wait for it to germinate. When the bud grows to 1-3cm, cut off the stem tip, peel off the outer 2-3 layers of leaves, wash it with running water for about 10 minutes, soak it in 75% alcohol on the ultra-clean table for 30 se...
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The invention discloses a rapid purification and rejuvenation method for local varieties of taros, which belongs to the field of biotechnologies. In a taro harvest season, stem tips of single plants are taken as explants and are induced so as to form tissue cultured seedlings; the tissue cultured seedlings are subjected to virus identification, and non-toxic tissue cultured seedlings are subjected to 2-generation propagation so as to form detoxified tissue cultured seedling strains; rooted detoxified tissue cultured seedlings are transplanted to a hypocaust matrix seedbed by strain to grow, so that core seed taros are harvested; the detoxified tissue cultured seedling strains are subjected to test seed identification, detoxified tissue cultured seedling strains with varietal typical characteristics are retained, and seed taros of the detoxified tissue cultured seedling strains are mixed so as to form large-scale purification and rejuvenation strains; after the large-scale purification and rejuvenation strains are bred through a subsequent propagation system, the detoxified seed taros can be used for production. According to the invention, by using the characteristics such as rapid propagation, detoxification and controllable indoor environment of a tissue culture technique, the production of purified, rejuvenated and detoxified core seed taros of local varieties of Jingjiang taros, which is required to be completed in three years originally, is shortened to one year.
Description
technical field [0001] The "method for rapid purification and rejuvenation of local varieties of taro" of the present invention is to obtain test-tube seedlings from tissue culture of taro stem tips under the condition of isolation, and belongs to the field of biotechnology. Background technique [0002] Jiangsu Province has many varieties of local characteristic taro, which has good nutritional and health care quality, and contains a variety of vitamins. It can be used as food and vegetables. It is a tonic suitable for all ages. It has broad processing prospects and is famous and sold well Domestic and foreign markets. For a long time, the cultivation of taro in my country has been dominated by farmers' spontaneous and sporadic planting, and the taro seeds used are mainly farmers' self-reserved seeds. Many farmers use seeds in confusion, and their production is endangered by viral diseases. After long-term planting, many local varieties of taro have mixed degeneration and...
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IPC IPC(8): A01H4/00
Inventor 殷剑美张培通王立韩晓勇郭文琦李春宏
Owner JIANGSU ACAD OF AGRI SCI