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Evaluation method of safety of genetically modified insect resistant rice relative to predator hylyphantes graminicola

A kind of technology for the safety evaluation of the grasshopper spider, applied in the field of safety evaluation of the transgenic insect-resistant rice to the predatory natural enemy the grasshopper spider

Inactive Publication Date: 2014-07-23
HUAZHONG AGRI UNIV
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Problems solved by technology

[0003] However, while the use of insect-resistant transgenic plants brings benefits to humans, there are also certain potential ecological risks.

Method used

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  • Evaluation method of safety of genetically modified insect resistant rice relative to predator hylyphantes graminicola
  • Evaluation method of safety of genetically modified insect resistant rice relative to predator hylyphantes graminicola
  • Evaluation method of safety of genetically modified insect resistant rice relative to predator hylyphantes graminicola

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Embodiment Construction

[0024] The Bt gene-transformed insect-resistant rice of the present invention selects the cry2Aa gene-transferred rice T2A-1, which has high resistance to lepidopteran pests. The non-transgenic parents are selected for the Guangminghui 63 rice and cry2Aa gene-transferred rice T2A-1. Provided by the State Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University. Minghui 63 rice and cry2Aa transgenic rice T2A-1 were grown to a height of about 15 cm in Yoshida nutrient solution in a cultivation tray of length × width × height = 25 cm × 20 cm × 3 cm, and then used for evaluation experiments. The conditions for cultivating rice seedlings are temperature (26±2°C) and relative humidity (80%).

[0025] The tested brown planthoppers of the present invention were collected from paddy fields in Wuhan, Hubei, and established populations on rice seedlings of T2A-1 and Minghui 63 respectively indoors, and reared for more than 10 generations. Female adult spiders of Dros...

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Abstract

The invention relates to an evaluation method of safety of genetically modified insect resistant rice relative to predator hylyphantes graminicola. The evaluation method is composed of a three-level nutrition test part, a protein transfer rule part, a predation function reaction part and a population density and population dynamics comparison part. The influence of Bt-gene modified insect resistant rice on the hylyphantes graminicola is evaluated through the food chain of the hylyphantes graminicola; the way and degree that the hylyphantes graminicola is exposed in Bt proteins are identified through an ELISA detection means; the influence of the Bt-gene modified insect resistant rice on a non-target organism predation function is evaluated; the Bt-gene modified insect resistant rice and non-genetically-modified parent are compared, and the influence on the hylyphantes graminicola population is evaluated; finally, the ecological consequences generated by genetically modified crop cultivation on non-target organisms are evaluated synthetically according to the data of the four parts. The evaluation method is systematic and scientific, and has great significance for the research of the influence of genetically modified crops on the non-target organisms and ecological safety of the genetically modified crop cultivation.

Description

technical field [0001] The invention belongs to the technical field of ecological risk assessment of transgenic crops, and in particular relates to a method for evaluating the safety of transgenic insect-resistant rice against the predatory natural enemy Auranthus praecox. Background technique [0002] Rice is one of the most important food crops in the world, and more than 3 billion people in the world depend on rice for food. At the same time, rice is one of the most pest-damaged food crops. Every year, due to the damage of rice leaf rollers, borer borers and other Lepidoptera insects, major yield losses are caused (Cheng, 1996; Nathan, 2006). In actual production, the control of lepidopteran pests has long relied on chemical pesticides. The abuse of chemical pesticides not only increases the production cost of rice, but also pollutes the environment. It is also easy to cause pests to develop resistance and reduce the efficacy of pesticides (Su et al. , 2003). Therefore,...

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Application Information

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IPC IPC(8): A01K67/033G01N33/68
Inventor 华红霞韩宇何月平赵景蔡万伦
Owner HUAZHONG AGRI UNIV
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