Primers and probes used for human drug-induced deafness gene mutation screening, and application methods thereof

Abstract

The invention discloses primers and probes used for human drug-induced deafness gene mutation screening and application methods thereof, and belongs to the field of gene detection technology. The invention discloses 32 compositions of the primers and the probes used for human drug-induced deafness gene mutation screening, and applications methods of the 32 compositions of the primers and the probes used for human drug-induced deafness gene mutation screening in human drug-induced deafness gene mutation screening. The primers and the probes used for human drug-induced deafness gene mutation screening, and the application methods thereof can be used for detection of a plurality of samples in one PCR reaction; compared with traditional detection method, accuracy is high; steps are few; and time consumption is less.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and particularly relates to primers, probes and application methods for screening human drug-induced deafness gene mutations. Background technique [0002] Deafness is a common disease that seriously affects human health. It is also one of the most common clinical genetic diseases. Because of its complex etiology, high incidence and difficult treatment, it greatly affects the patient's interpersonal communication and quality of life. According to statistics, the incidence of deafness in newborns is about 1‰ to 3‰, and about 70 million people in the world suffer from different degrees of hearing loss. my country is the country with the largest population of hearing impairments in the world. Among people, hearing disabilities account for about 24% of the total, involving tens of millions of families across the country. [0003] There are many causes of deafness, including genetic factors and...

AI Technical Summary

Problems solved by technology

Gene chip technology is used for the detection of genetic deafness-causing genes, which has the advantages of high throughput and high degree of automation, but has disadvantages such as poor repeatability, easy cross-contamination, and false positive results.

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