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Method for improving festuca arundinacea drought resistance by adopting dried sludge fungicide

A technology for air-drying sludge and tall fescue, which is applied in the fields of botanical equipment and methods, chemicals for biological control, bacteria, etc., and can solve problems that have not yet been reported.

Inactive Publication Date: 2014-07-30
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few researches on the drought resistance of the composite microbial agent of Pseudomonas, Trichoderma and Streptomyces, and the technology of its application to the drought resistance of lawn plants has not yet been reported.

Method used

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  • Method for improving festuca arundinacea drought resistance by adopting dried sludge fungicide
  • Method for improving festuca arundinacea drought resistance by adopting dried sludge fungicide
  • Method for improving festuca arundinacea drought resistance by adopting dried sludge fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] (1) Cultivation of strains

[0109] Weigh fresh sludge and prepare 10 by dilution separation method -3 、10 -4 、10 -5 、10 -6 sludge suspension. According to the isolated microorganisms, take different concentrations of sludge suspension (fungi take 10 -3 、10 -4 , Actinomycetes take 10 -4 、10 -5 , bacteria take 10 -5 、10 -6 ), shake well and pipette 0.2 mL into the prepared plate. Fungi use Martin's agar plate; actinomycetes use Gao's No. 1 agar plate; bacteria use beef extract peptone plate. Spread evenly with a sterilized applicator, repeat 3 times for each treatment, and incubate at 28°C. According to the shape, size, color and growth speed of the colonies in the plate, pick as many single colonies as possible from each sample and streak for purification. The strains were classified and counted according to their colony culture traits and morphological characteristics. The dominant strains were determined and stored under serial numbers pending further id...

Embodiment 2

[0118] A method for improving the drought resistance of tall fescue by air-dried sludge inoculum, characterized in that it is carried out according to the following steps:

[0119] (1) Selection of materials:

[0120] Select tall fescue with plump and uniform grains ( Festuca arundinacea L .) Seeds are the test materials; the test soil is ordinary garden soil, pH 7.44, saturated water content 0.58 ml / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, total potassium 45.61g / kg;

[0121] (2) Preparation method

[0122] Preparation of composite microbial bacterial agent: select Pseudomonas stutzeri, Trichoderma reesei and Streptomyces griseus flavum, purify and cultivate the isolated strains for 2 generations, take activated strains, and insert them into a container containing 200 mL of liquid medium. In a triangular flask, cultivate at a suitable temperature of 180 r / min, select 600 nm wavelength for turbidity measurement, take the OD600 nm value...

Embodiment 3

[0126] An air-dried sludge bacterial agent is characterized in that it is composed of the following components:

[0127] 100-fold dilution of Pseudomonas stutzeri + Trichoderma reesei + Streptomyces flavum; wherein the ratio of Pseudomonas stutzeri: Trichoderma reesei: Streptomyces flavum is 1:1:1; 3 bacteria The number of colonies of species is (2.15±0.03)×10 9 / ml~ (2.07±0.07)×10 9 / ml, the OD600 nm value of Pseudomonas is 0.592, according to the following steps:

[0128] (1) Selection of materials:

[0129] Select tall fescue with plump and uniform grains ( Festuca arundinacea L .) Seeds are the test materials; the test soil is ordinary garden soil, pH 7.44, saturated water content 0.58 ml / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, total potassium 45.61g / kg;

[0130] (2) Preparation method

[0131] Preparation of composite microbial bacterial agent: select Pseudomonas stutzeri, Trichoderma reesei and Streptomyces griseus flavum, p...

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Abstract

The invention discloses a method for improving festuca arundinacea drought resistance by adopting a dried sludge fungicide. The raw material used in the method is a 100-fold diluent of pseudomonas stutzeri, trichoderma reesei and yellow streptomycete or a 200-fold diluent of pseudomonas stutzeri, trichoderma reesei and yellow streptomycete. The research result shows that the complex microbial agent can form a good symbiotic relationship with a lawn plant; the complex microbial agent can enhance the drought resistance of the plant and promote plant growth by direct action of promoting absorption of host plant roots on the soil moisture and by indirect action of improving physiological and metabolic activity inside a plant body and improving the protective enzyme activity, and the water-saving percentage of the 200-fold diluent of the pseudomonas stutzeri, the trichoderma reesei and the yellow streptomycete can be up to 57.39%.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for improving the drought resistance of tall fescue with an air-dried sludge bacterial agent. Background technique [0002] The construction of a lawn planting system is one of the important contents that must be considered in the ecological construction of a modern city. It is a powerful manifestation of the social development and economic strength of a modern city. All aspects play a role that cannot be ignored, and the quality and area of ​​lawn greening in the city have become an important objective standard for evaluating the quality of the urban environment. Lawn greening is also increasingly subject to people's attention. [0003] The lawn plays a good role in beautifying, protecting and improving the environment on which human beings depend. However, turfgrass is an open plant system that exists in nature. Although it has a wide geographical dist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00C12N1/20C12N1/14
Inventor 多立安赵树兰王志旭
Owner TIANJIN NORMAL UNIVERSITY
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